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Ncbp3 (NM_025818) Mouse Tagged ORF Clone
CAT＃: MR209440
- TrueORF®
1200014J11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik)
ORF Plasmid: tGFP
"NM_025818" in other vectors (4)
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CNY 4,576.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200014J11Rik; C78393; C130061O14Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209440 representing NM_025818
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGTCCGCGGCCTGCGAGTGTCTGTGAAGGCGGAGGCCCCCGCTGGGCCGGCTTTGGGGCTTC CGTCGCCCGAGGTGGAGTCCGGTTTGGAGCGTGGCGAGCCGGAGCCCATGGAAGTGGAAGAAGGCGAGCT GGAAATCGTGCCGGTGCGGCGCTCCCTAAAGGAACTGCTCCCGGACACAAGCAGAAGATATGAAAACAAG GCTGGCAGCTTCATCACTGGGATCGATGTCACCTCCAAGGAAGCAATTGAAAAGAAAGAACAGCGAGCCA AGCGCTTCCACTTTCGAGCAGAAGTAAATCTTGCCCAGAGAAATGTAGCCTTGGACCGAGACATGATGAA GAAAGCAATTCCCAAGGTGAGACTGGAGACAATCTACATTTGTGGAGTGGATGAAATGAGCACCCAGGAT ATCTTCTCCTATTTTAAGGAATATCCTCCAGCTCACATTGAGTGGTTGGATGACACCTCCTGCAATGTGG TTTGGCTGGACGAGATGACGGCCACACGAGCTCTCATCAATATGAGTTCTCTGCCAGCCCAGGATAAGAT GAGAAGCAGAGATGCCAGTGAAGACAAGTCGTCGGAGAAGAACAAGAAAGACAAGCAGGAAGACAGTTCA GATGACGACGAGACTGAAGAAGGAGAAGTGGAAGATGAGAATTCAAGTGATGTAGAGTTGGACACGTTGT CTCAGGTAGAAGAAGAGTCTCTGTTACGAAATGATCTTCGTCCAGCTAACAAACTTGCTAAAGGAAATAG ACTATTCATGAGATTTGCTACAAAAGATGACAAAAAGGAACTTGGAGCAGCCAGAAGAAGTCAATACTAC ATGAAGTATGGAAATCCAAATTATGGAGGCATGAAAGGAATTCTTAGCAATTCATGGAAGCGAAGATATC ATTCCCGTCGCATTCAGCGGGATGTGATCAAGAAGAGAGCCCTGATTGGGGATGACGTTGGCTTGACGTC GTATAAACACCGACATTCCGGATTAGTGAATGTTCCTGAGGAACCCATTGAGGAAGAGGAGGAGGAGGAG GAGGAAGAGGAGGACCAGGACATGGATGCCGATGACAGAGTCGTGGTGGAGTACCATGAGGAGCTCCCAG GTCTCAAGCAGCCCCGTGAGCGGAGCCTGTCTAGGCGATCCAGTGCGAGCAGCTCAGACTCAGATGAGAT GGACTATGATCTGGAACTGAAGATGATTTCTACTCCCTCCCCAAAGAAAAGCATGAAAATGACCATGTAT GCAGATGAAGTAGAGTCCCAACTGAAAAGTATTAGGAATCCCATGAGGGCAGATAGTATATCCACAAGCA ATATAAAAAACCGAATTGGTAACAAATTGCCTCCTGAGAAATTTGCAGATGTCCGTCATCTCTTAGATGA AAAGAGGCAGCATTCCTGTCCACGGCCAGCAGTCAGCAGTACTAAACCAGATATTCGCCAGCGGTTAGGA AAAAGACCGTATTCTCCAGAGAAGGCTTTTAGTAGTAACCAGGTGGTTCGGAGAGAGCCCTCTTCAGATG TACATAGTAGGCTAGGTGTTCCCAGGCAAGATGTTAAAGGCCTCTACTCTGATACTCGGGAGAGGAAATC AGGTGGTTTGTGGACTCGCTTAGGATCTACACCCAAGACCAAAGAAAAGAACACGAAGAAAGTGGATCAC AGGGCATCTGGTGCTGAGGAAGATGACTCGGAGCTTCAGAGAGCATGGGGTGCTCTGATCAAGGAGAAAG AAGAGTCTCGCCAGAAGAAGAGTCGGCTGGATAGCTTGCCTTCGCTCCAGATTGAAGTCAGCCGGGAAAG TAGCTCTGGTTCAGAGGCAGAGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209440 representing NM_025818
Red=Cloning site Green=Tags(s) MAAVRGLRVSVKAEAPAGPALGLPSPEVESGLERGEPEPMEVEEGELEIVPVRRSLKELLPDTSRRYENK AGSFITGIDVTSKEAIEKKEQRAKRFHFRAEVNLAQRNVALDRDMMKKAIPKVRLETIYICGVDEMSTQD IFSYFKEYPPAHIEWLDDTSCNVVWLDEMTATRALINMSSLPAQDKMRSRDASEDKSSEKNKKDKQEDSS DDDETEEGEVEDENSSDVELDTLSQVEEESLLRNDLRPANKLAKGNRLFMRFATKDDKKELGAARRSQYY MKYGNPNYGGMKGILSNSWKRRYHSRRIQRDVIKKRALIGDDVGLTSYKHRHSGLVNVPEEPIEEEEEEE EEEEDQDMDADDRVVVEYHEELPGLKQPRERSLSRRSSASSSDSDEMDYDLELKMISTPSPKKSMKMTMY ADEVESQLKSIRNPMRADSISTSNIKNRIGNKLPPEKFADVRHLLDEKRQHSCPRPAVSSTKPDIRQRLG KRPYSPEKAFSSNQVVRREPSSDVHSRLGVPRQDVKGLYSDTRERKSGGLWTRLGSTPKTKEKNTKKVDH RASGAEEDDSELQRAWGALIKEKEESRQKKSRLDSLPSLQIEVSRESSSGSEAES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025818 |
| ORF Size | 1845 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025818.3, NP_080094.3 |
| RefSeq Size | 6284 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 66874 |
| UniProt ID | Q8BZR9 |
| MW | 70.5 kDa |
| Gene Summary | Associates with NCBP1/CBP80 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. NCBP3 serves as adapter protein linking the capped RNAs (m7GpppG-capped RNA) to NCBP1/CBP80. Unlike the conventional CBC with NCBP2 which binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus, the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role in only mRNA export. The alternative CBC is particularly important in cellular stress situations such as virus infections and the NCBP3 activity is critical to inhibit virus growth (PubMed:26382858).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219729 | 1200014J11Rik (untagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik), (10ug) |
CNY 7,890.00 |
|
| MG209440 | 1200014J11Rik (tGFP-tagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik) |
CNY 5,040.00 |
|
| MR209440L3 | Lenti ORF clone of 1200014J11Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik) |
CNY 6,460.00 |
|
| MR209440L4 | Lenti ORF clone of 1200014J11Rik (mGFP-tagged) - Mouse RIKEN cDNA 1200014J11 gene (1200014J11Rik) |
CNY 6,460.00 |
