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Numbl (NM_010950) Mouse Tagged ORF Clone
CAT＃: MR209340
- TrueORF®
Numbl (Myc-DDK-tagged) - Mouse numb-like (Numbl)
ORF Plasmid: tGFP
"NM_010950" in other vectors (4)
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CNY 4,504.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | nbl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209340 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGCAGCGCGGCGGCCAGCGGCGGACCCAGGAGACCTGATCAGCATTTGTCCCCAGCCCCCTGTG GGGCCTCGGGGCCCCCTGAAACCTTCAGGACGGAGTCAGACGGGGCAGGCACCATGAACAAGTTACGGCA GAGTCTGCGGCGGAGGAAGCCAGCCTATGTGCCTGAGGCATCACGCCCACACCAGTGGCAGGCAGATGAG GATGCGGTGCGCAAAGGCACCTGCAGCTTCCCTGTTAGGTACCTGGGCCACGTGGAAGTGGAGGAGTCCC GGGGAATGCACGTTTGTGAAGATGCTGTGAAGAAGCTGAAGGCGATGGGCCGGAAGTCCGTGAAGTCTGT CCTGTGGGTGTCAGCTGATGGGCTCCGAGTGGTGGATGACAAGACCAAGGACCTGCTGGTAGACCAGACC ATTGAGAAGGTCTCCTTCTGTGCTCCTGATCGCAACCTGGACAAGGCTTTCTCCTACATATGCCGCGACG GCACCACACGCCGCTGGATCTGCCACTGCTTCCTGGCCCTCAAGGACTCTGGTGAGAGGCTGAGCCATGC TGTGGGCTGCGCGTTCGCCGCCTGCCTGGAGAGGAAGCAGCGACGGGAGAAGGAATGCGGAGTCACCGCC GCCTTCGACGCCAGTCGCACCAGCTTTGCCCGTGAGGGCTCCTTCCGCCTGTCGGGGGGCGGGCGGCCTG CGGAGCGCGAGGCAGGGGACAAGAAGAAAGCCGAAGCAGCAGCTGCTCCTGCTGTGGCTCCTGGACCTGC CCAGCCTGGGCACGTTTCTCCGACACCAGCCACCACATCCCCTGGAGAGAAGGGAGAGGCGGGCACCCCA GTGGCTGCAGGCACCACTGCTGCTGCCATTCCCCGGCGCCACGCACCTCTGGAGCAGCTGGTTCGCCAGG GCTCCTTTCGTGGGTTCCCTGCCCTCAGTCAGAAGAACTCACCTTTCAAACGTCAGCTGAGCCTACGGTT GAATGAGCTGCCATCAACACTGCAGCGCCGCACGGACTTCCAGGTGAAGGGCACAGTGCCTGAGATGGAG CCTCCTGGTACCGGGGACAGCGATGGCATCAATGCTCTGTGCACACAGATCAGCTCGTCTTTCGCCAGCG CTGGGGCCCCAGCGTCAGGGCCACCACCTGCCACAACAGGGACTTCTGCCTGGGGTGAGCCCTCCGTACC CGCTGCAGCTGCCTTCCAGCCTGGGCACAAGCGGACACCCTCTGAGGCTGAGCGATGGTTGGAGGAAGTG TCCCAGGTGGCTAAGGCCCAGCAGCAACAGCAACAGCAGCAGCAGCAACAACAGCAGCAGCAAGCCACCT CCGTGCCACCAATGCCCACCATGGCCCCCACCCTTCAACCCTTTTCTGCCCCAGTGGGGCCCTTTGACAC TGCAGCTGCCCAAGTGGCTGTTTTCCTGCCGCCCACACATATGCAGCCTCCGTTTGTGCCCGCCTACCCA GGTCTGGGTTATCCACCAATGCCCCGGGTGCCTGTGGTGGGCATCACACCTTCACAGATGGTAGCCAACG CCTTCTGCTCAGCTGCCCAGCTCCAGCCCCAACCTGCCACACTGCTTGGAAAAGCTGGGGCCTTCCCCCC ACCTGCTGCACCCAGTGCCCCTGGGGGCCAGGCCCGTCCGCGCCCCAATGGGGCTCCCTGGCCCCCAGAG CCAGCGCCTGCCCCTGCCCCTGAGTTGGACCCCTTCGAGGCCCAGTGGGCGGCACTAGAAGGCAAACCCG CTGTGGAGAAACCCTCCAACCCCTTCTCTGGTGACTTGCAGAAGACCTTCGAGATTGAACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209340 protein sequence
Red=Cloning site Green=Tags(s) MSRSAAASGGPRRPDQHLSPAPCGASGPPETFRTESDGAGTMNKLRQSLRRRKPAYVPEASRPHQWQADE DAVRKGTCSFPVRYLGHVEVEESRGMHVCEDAVKKLKAMGRKSVKSVLWVSADGLRVVDDKTKDLLVDQT IEKVSFCAPDRNLDKAFSYICRDGTTRRWICHCFLALKDSGERLSHAVGCAFAACLERKQRREKECGVTA AFDASRTSFAREGSFRLSGGGRPAEREAGDKKKAEAAAAPAVAPGPAQPGHVSPTPATTSPGEKGEAGTP VAAGTTAAAIPRRHAPLEQLVRQGSFRGFPALSQKNSPFKRQLSLRLNELPSTLQRRTDFQVKGTVPEME PPGTGDSDGINALCTQISSSFASAGAPASGPPPATTGTSAWGEPSVPAAAAFQPGHKRTPSEAERWLEEV SQVAKAQQQQQQQQQQQQQQQATSVPPMPTMAPTLQPFSAPVGPFDTAAAQVAVFLPPTHMQPPFVPAYP GLGYPPMPRVPVVGITPSQMVANAFCSAAQLQPQPATLLGKAGAFPPPAAPSAPGGQARPRPNGAPWPPE PAPAPAPELDPFEAQWAALEGKPAVEKPSNPFSGDLQKTFEIEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010950 |
| ORF Size | 1815 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010950.2, NP_035080.2 |
| RefSeq Size | 2733 bp |
| RefSeq ORF | 1815 bp |
| Locus ID | 18223 |
| UniProt ID | O08919 |
| MW | 64.1 kDa |
| Gene Summary | Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202603 | Numbl (untagged) - Mouse numb-like (Numbl), (10ug) |
CNY 2,000.00 |
|
| MG209340 | Numbl (tGFP-tagged) - Mouse numb-like (Numbl) |
CNY 5,040.00 |
|
| MR209340L3 | Lenti ORF clone of Numbl (Myc-DDK-tagged) - Mouse numb-like (Numbl) |
CNY 6,460.00 |
|
| MR209340L4 | Lenti ORF clone of Numbl (mGFP-tagged) - Mouse numb-like (Numbl) |
CNY 6,460.00 |
