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Mtf2 (NM_013827) Mouse Tagged ORF Clone
CAT＃: MR209240
- TrueORF®
Mtf2 (Myc-DDK-tagged) - Mouse metal response element binding transcription factor 2 (Mtf2)
ORF Plasmid: tGFP
"NM_013827" in other vectors (4)
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CNY 4,416.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9230112N11Rik; AA537621; C76717; M96; Pcl2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209240 representing NM_013827
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGACTCTACAGGAGCAGGTAATTCACTGGTCCACAAGCGGTCTCCTTTACGTCGAAACCAAAAGA CCTCAGCATCCTTGAACAAGCTGTCTTTACAGGATGGACATAAAGCCAAAAAACCAGCATGTAAATTTGA AGAGGGTCAAGATGTCTTAGCTAGATGGTCAGATGGCTTGTTTTATCTTGGAACTATCAAAAAGATAAAC ATATTGAAACAGAGCTGCTTCATCATATTTGAGGACAGTTCCAAATCCTGGGTTCTCTGGAAGGACATCC AAACAGGAGCCACTGGGAGTGGGGAAATGGTCTGTACAATATGTCAAGAAGAGTATTCAGAAGCTCCCAA TGAAATGGTTATATGTGATAAGTGTGGTCAAGGATATCATCAGCTGTGTCACACACCTCATATTGACTCG AGTGTGATTGATTCAGATGAAAAGTGGCTTTGTCGACAGTGCGTTTTTGCAACAACTACAAAGAGGGGTG GTGCGCTTAAGAAAGGACCAAATGCCAAAGCATTGCAAGTCATGAAGCAGACATTACCCTATAGTGTGGC TGACCTTGAATGGGATGCAGGCCATAAAACCAATGTCCAGCAGTGCTACTGCTATTGTGGAGGCCCTGGA GACTGGTATTTAAAGATGCTGCAGTGCTGCAAATGTAAGCAGTGGTTTCATGAAGCTTGTGTCCAATGCC TTCAGAAGCCAATGCTGTTTGGAGATAGGTTTTATACATTTATTTGCTCTGTCTGCAGTTCTGGACCAGA ATACCTCAAACGTCTACCGTTACAGTGGGTAGATATAGCACACCTATGCCTTTACAACCTAAGTGTTATT CACAAGAAGAAATACTTTGATTCTGAACTTGAGCTTATGACATACATTAATGAAAACTGGGATAGATTGC ACCCTGGAGAGCTGGCAGACACACCCAAATCTGAAAGATATGAGCATGTTCTGGAGGCATTAAATGATTA CAAGACCATGTTTATGTCTGGGAAAGAAATAAAGAAGAAGAAGCATTTGTTTGGGTTGCGAATTCGTGTT CCTCCTGTGCCACCAAATGTGGCTTTCAAAGCAGAGAAAGAACCAGAAGGAACATCCCATGAATTTAAAA TTAAAGGCAGAAAGGCATCCAAACCTACATCTGACTCAAGGGAAGTAAGCAATGGGATAGAAAAAAAAGG AAAGAAAAAATCTGTAGGTCGTCCTCCTGGCCCATATACAAGAAAAATGATTCAAAAAACTGCTGAGCTA CCTTTGGATAAGGAATCAGTTTCAGAGAATCCTACGTTGGATTTACCTTGTTCTATAGGGAGAACTGAGG GAATTGCACATTCATCCAATACCTCAGATGTGGACCTCACGGGTGCTTCCAGTGCAAACGAAACTACCTC GGCTAGCATTTCCAGGCATTGTGGATTATCAGACTCTAGAAAAAGAACTCGCACAGGAAGATCATGGCCT GCTGCAATACCACATTTGCGGAGAAGAAGAGGTCGCCTTCCACGAAGAGCACTCCAGACTCAGAACTCAG AAGTTGTGAAAGATGATGAAGGCAAAGAAGATTATCAGTTTGAAGAACTTAACACAGAAATTCTGAATAA CTTAGCAGATCAGGAGTTGCAGCTCAATCACCTGAAAAACTCCATCACTAGTTATTTTGGTGCCGCAGGT AGAATAGCATGTGGCGAAAAATACAGAGTTTTGGCTCGTCGGGTGACGCTTGATGGGAAGGTGCAGTATC TTGTGGAATGGGAAGGAGCGACTGCATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209240 representing NM_013827
Red=Cloning site Green=Tags(s) MRDSTGAGNSLVHKRSPLRRNQKTSASLNKLSLQDGHKAKKPACKFEEGQDVLARWSDGLFYLGTIKKIN ILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDS SVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPG DWYLKMLQCCKCKQWFHEACVQCLQKPMLFGDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVI HKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRV PPVPPNVAFKAEKEPEGTSHEFKIKGRKASKPTSDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEL PLDKESVSENPTLDLPCSIGRTEGIAHSSNTSDVDLTGASSANETTSASISRHCGLSDSRKRTRTGRSWP AAIPHLRRRRGRLPRRALQTQNSEVVKDDEGKEDYQFEELNTEILNNLADQELQLNHLKNSITSYFGAAG RIACGEKYRVLARRVTLDGKVQYLVEWEGATAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013827 |
| ORF Size | 1779 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013827.3, NP_038855.2 |
| RefSeq Size | 4343 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 17765 |
| UniProt ID | Q02395 |
| MW | 66.9 kDa |
| Gene Summary | Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex, leading to enhance PRC2 H3K27me3 methylation activity. Regulates the transcriptional networks during embryonic stem cell self-renewal and differentiation. Promotes recruitment of the PRC2 complex to the inactive X chromosome in differentiating XX ES cells and PRC2 recruitment to target genes in undifferentiated ES cells. Required to repress Hox genes by enhancing H3K27me3 methylation of the PRC2 complex. In some conditions may act as an inhibitor of PRC2 activity: able to activate the CDKN2A gene and promote cellular senescence by suppressing the catalytic activity of the PRC2 complex locally. Binds to the metal-regulating-element (MRE) of MT1A gene promoter.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219390 | Mtf2 (untagged) - Mouse metal response element binding transcription factor 2 (Mtf2), (10ug) |
CNY 4,424.00 |
|
| MG209240 | Mtf2 (tGFP-tagged) - Mouse metal response element binding transcription factor 2 (Mtf2) |
CNY 5,800.00 |
|
| MR209240L3 | Lenti ORF clone of Mtf2 (Myc-DDK-tagged) - Mouse metal response element binding transcription factor 2 (Mtf2) |
CNY 7,130.00 |
|
| MR209240L4 | Lenti ORF clone of Mtf2 (mGFP-tagged) - Mouse metal response element binding transcription factor 2 (Mtf2) |
CNY 7,130.00 |
