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Grk5 (NM_018869) Mouse Tagged ORF Clone
CAT＃: MR209199
- TrueORF®
Grk5 (Myc-DDK-tagged) - Mouse G protein-coupled receptor kinase 5 (Grk5)
"NM_018869" in other vectors (2)
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CNY 4,400.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gprk5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209199 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGAAAACATCGTGGCCAACACGGTCTTGCTGAAAGCCCGGGAAGGGGGTGGAGGAAAGCGCA AAGGGAAAAGCAAGAAGTGGAAGGAAATCCTGAAGTTTCCTCACATCAGCCAGTGTGAAGACCTCCGAAG GACCATAGACAGAGATTACTACAGTCTCTGTGACAAGCAACCAATTGGGAGACTGCTTTTTCGACAGTTC TGTGAAACCAGGCCTGGGCTGGAGTGCTACATTCAGTTCCTGGACTTAGTGGCAGAATATGAAATTACTC CAGATGAAAACCTTGGGGCGAAGGGGAAGGAAATAATGACCAAGTACCTCACTCCAAAGTCCCCAGTCTT CATTGCCCAAGTTGGACAGGACCTGGTCTCCCAGACAGAGAAGAAGCTCCTGCAGAGCCCCTGCAAAGAA CTCTTCTCTGCTTGTGCTCAGTCTGTCCATGACTACTTGACGGGAGACCCCTTCCACGAGTACCTGGATA GCATGTATTTTGACCGTTTTCTGCAGTGGAAATGGTTAGAAAGACAACCAGTGACCAAAAACACTTTCCG GCAGTACCGAGTGCTGGGCAAAGGGGGCTTTGGAGAGGTCTGTGCCTGCCAGGTTCGGGCCACTGGTAAA ATGTATGCTTGTAAACGCTTAGAGAAGAAGAGGATCAAAAAGAGGAAAGGCGAATCCATGGCACTCAACG AAAAGCAGATTCTTGAGAAGGTCAACAGCCAGTTTGTGGTCAACCTGGCCTATGCCTATGAAACCAAAGA TGCACTATGCCTGGTTCTGACCATTATGAATGGTGGTGACCTGAAGTTTCACATCTACAATATGGGGAAT CCTGGCTTTGAGGAAGAGCGAGCCTTATTTTATGCAGCTGAGATCCTCTGTGGCCTAGAAGACTTACACC GTGAGAACACTGTCTATAGAGATCTAAAACCCGAAAACATCTTGCTGGATGATTATGGCCACATAAGGAT CTCAGACCTCGGACTGGCCGTGAAGATCCCCGAGGGAGACCTTATCCGTGGCCGGGTAGGCACTGTTGGC TACATGGCCCCAGAAGTTCTGAACAACCAGCGATATGGACTGAGCCCTGACTACTGGGGCCTGGGCTGCC TCATCTATGAGATGATTGAAGGCCAGTCACCATTTCGAGGTCGCAAGGAGAAGGTTAAGCGGGAAGAGGT GGATCGCCGGGTGCTGGAGACTGAGGAAGTATATTCCTCCAAGTTCTCTGAAGAGGCCAAGTCCATCTGC AACATGCTGCTCACCAAAGACTCGAAGCAGAGGCTGGGCTGCCAGGAGGAGGGAGCCGCCGAGGTCAAGA GGCACCCCTTCTTCAGGAACATGAACTTTAAGCGCCTGGAGGCTGGGATGTTGGACCCTCCCTTCGTTCC AGATCCCCGGGCTGTATACTGCAAGGATGTGCTGGACATTGAGCAGTTCTCCACTGTGAAAGGTGTCAAC CTGGACCATACGGACGATGATTTTTACTCAAAGTTCTCTACAGGCTCTGTGCCAATTCCATGGCAAAATG AGATGATAGAAACAGAATGTTTCAAGGAGCTGAATGTGTTCGGACCTAACGGTACCCTCTCACCAGACCT GAACAGAAGTCAGCCTCCAGAACCGCCAAAGAAAGGGCTGTTCCACAGACTCTTCAGGCGGCAGCATCAA AGCAATTCCAAGAGTTCACCTACTCCTAAGACCAGTTGTAACCACCGAATAAATTCAAACCACATCAATT CAAACTCCACTGGAAGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209199 protein sequence
Red=Cloning site Green=Tags(s) MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYYSLCDKQPIGRLLFRQF CETRPGLECYIQFLDLVAEYEITPDENLGAKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEKKLLQSPCKE LFSACAQSVHDYLTGDPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK MYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGN PGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVG YMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSSKFSEEAKSIC NMLLTKDSKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVN LDHTDDDFYSKFSTGSVPIPWQNEMIETECFKELNVFGPNGTLSPDLNRSQPPEPPKKGLFHRLFRRQHQ SNSKSSPTPKTSCNHRINSNHINSNSTGSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018869 |
| ORF Size | 1773 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018869.2 |
| RefSeq Size | 3182 bp |
| RefSeq ORF | 1773 bp |
| Locus ID | 14773 |
| UniProt ID | Q8VEB1 |
| MW | 67.7 kDa |
| Gene Summary | Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209199L3 | Lenti ORF clone of Grk5 (Myc-DDK-tagged) - Mouse G protein-coupled receptor kinase 5 (Grk5) |
CNY 7,130.00 |
|
| MR209199L4 | Lenti ORF clone of Grk5 (mGFP-tagged) - Mouse G protein-coupled receptor kinase 5 (Grk5) |
CNY 7,130.00 |
