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Gbp5 (NM_153564) Mouse Tagged ORF Clone
CAT＃: MR209198
- TrueORF®
Gbp5 (Myc-DDK-tagged) - Mouse guanylate binding protein 5 (Gbp5)
ORF Plasmid: tGFP
"NM_153564" in other vectors (4)
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CNY 4,400.00
CNY 5,230.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5330409J06Rik; Gbp5a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209198 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCAGAGATTCACATGCCAGAACCCTTGTGCCTCATTGGGAGCACCGAGGGACACCTAGTAACTA ACCAGGAAGCCCTGAAGATTCTGTCTGCCATCACACAGCCAGTGGTGGTGGTAGCCATTGTGGGTCTTTA TCGCACAGGCAAATCCTACCTGATGAACAAGCTGGCTGGGAAGGAGAAGGGCTTTTCTGTTGGATCCACT GTACAGTCTCACACCAAGGGGATCTGGATGTGGTGTGTGCCTCACCCCCAAAAGCCAGACCACACTTTGG TTCTGCTTGACACTGAAGGCCTGGGAGATGTGGAGAAGGATGATAAAAAGAATGATACTCAGATCTTTGC ACTAGCAATCCTCCTCAGTAGCACCTTTGTATACAACACTATGAACAAAATTGACCAGGGGGCTATCGAC CTATTGCACAATGTGACAGAACTGACAGACTTGCTCCGGACAAGAAACTCCTCTGATTCTAATCAAACTG AGGGTGAGGGACCTGCTGACATGAGCTTCTTCCCAGACTTGGTGTGGACTCTGAGAGATTTCTTCCTGGA CCTGCAAGCCAATGGGCATGCCATCACATCAGATGAATATCTGGAGAATTCACTGAAGCTGAAGCAAGGT AGCGATGAAAGAACTCAAACATTCAATCTACCGCGGCTGTGCATACAGAAGTTCTTCCCAGTAAAGAAAT GCTTTGTTTTTGACGCTCCTGCGCTTGGAAGTAAGCTTTCCCAGCTTCCAACACTCAGCAACGAGGAGCT GAATTCAGATTTTGTGCAGGACCTTTCAGAATTCTGTTCACACATCTTCACCCAATCTAAGACCAAGACT CTTCCAGGAGGCATCCAGGTCAACGGACCTCGTCTAGAAAGCCTGGTGCTGACTTATGTCGATGCCATCA ACAGCGGGGCTCTGCCTTCCATAGAGAACACAGTAGTAACCTTGGCCCGGAGGGAGAACTCTGCAGCAGT TCAAAAGGCCATTGGTCACTACGATCAGCTGATGAGCGAGAAGGTGCAGCTGCCCACAGAGACCCTCCAG GAGCTGCTGGACCTGCACCGGACCTGTGAGCGAGAGGCCATAGAAATCTTCAGGAAGCATTCGTTCAAGG ATGAGGGTGAATTTTTCCAGAAAGAATTGGAGAGCCTACTAAGTGCAAAGCAGGATGAGATTTGTAAGAA GAACGCGGATGCTTCTGCAGCCCTCTGCTCAACCCTACTTGGGAGTATTTTTAAGCCTCTGGAACAAGAA GTGGCGCAGGAGTTCTATCATAAACCAGGAGGCCACAAGCTCTTCCTTCAGAGGATGGAACAGCTGAAGG CAAATTACCGTCAGCAGCCAGGGAAGGGAACACAGGCTGAGGAAGTGCTGCAGACCTATTTGAACGCCAA AGAAACAGTGAGCCGTACAATTCTACAAACAGACCAGGTTCTCACAGACAAGGAGATCCAGAAGAAAGCG GAACAAGAAAGAGCAGAGGCTGCCCGGCTCAAAGCACAGAGGTTGGAGGCTATTCGCATCCAGGAAGAGC AAAGGAAAGCAGAGATGGAGAGACAGCATCAAGAGCAATTGAGGCAAATAGCATTGGAGAAGGCAAGAGT TGCACAAGAGCAACAGTGGATCCTGAAGCAGAGAGCCCAGGAAGAGGCTGATAGAATCAAGGCAGAGCAG GAAGCTCAACTCAGAGCACTTCAACAGCAGCTCCAACACATGAGGGAAATGAACCACCACCGTAGACATC ATCATGACTGTGTTATAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209198 protein sequence
Red=Cloning site Green=Tags(s) MAPEIHMPEPLCLIGSTEGHLVTNQEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKEKGFSVGST VQSHTKGIWMWCVPHPQKPDHTLVLLDTEGLGDVEKDDKKNDTQIFALAILLSSTFVYNTMNKIDQGAID LLHNVTELTDLLRTRNSSDSNQTEGEGPADMSFFPDLVWTLRDFFLDLQANGHAITSDEYLENSLKLKQG SDERTQTFNLPRLCIQKFFPVKKCFVFDAPALGSKLSQLPTLSNEELNSDFVQDLSEFCSHIFTQSKTKT LPGGIQVNGPRLESLVLTYVDAINSGALPSIENTVVTLARRENSAAVQKAIGHYDQLMSEKVQLPTETLQ ELLDLHRTCEREAIEIFRKHSFKDEGEFFQKELESLLSAKQDEICKKNADASAALCSTLLGSIFKPLEQE VAQEFYHKPGGHKLFLQRMEQLKANYRQQPGKGTQAEEVLQTYLNAKETVSRTILQTDQVLTDKEIQKKA EQERAEAARLKAQRLEAIRIQEEQRKAEMERQHQEQLRQIALEKARVAQEQQWILKQRAQEEADRIKAEQ EAQLRALQQQLQHMREMNHHRRHHHDCVIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153564 |
| ORF Size | 1773 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153564.1, NM_153564.2, NP_705792.2 |
| RefSeq Size | 3021 bp |
| RefSeq ORF | 1773 bp |
| Locus ID | 229898 |
| UniProt ID | Q8CFB4 |
| MW | 67 kDa |
| Gene Summary | As an activator of NLRP3 inflammasome assembly, plays a role in innate immunity and inflammation. Promotes selective NLRP3 inflammasome assembly in response to microbial and soluble, but not crystalline, inflammasome activating agents (PubMed:22461501). Hydrolyzes GTP, but in contrast to other family members, such as GBP1 and GBP2, does not produce GMP (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219463 | Gbp5 (untagged) - Mouse guanylate binding protein 5 (Gbp5), (10ug) |
CNY 7,510.00 |
|
| MG209198 | Gbp5 (tGFP-tagged) - Mouse guanylate nucleotide binding protein 5 (Gbp5) |
CNY 5,800.00 |
|
| MR209198L3 | Lenti ORF clone of Gbp5 (Myc-DDK-tagged) - Mouse guanylate binding protein 5 (Gbp5) |
CNY 7,130.00 |
|
| MR209198L4 | Lenti ORF clone of Gbp5 (mGFP-tagged) - Mouse guanylate binding protein 5 (Gbp5) |
CNY 7,130.00 |
