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C8a (NM_146148) Mouse Tagged ORF Clone
CAT＃: MR209163
- TrueORF®
C8a (Myc-DDK-tagged) - Mouse complement component 8, alpha polypeptide (C8a)
ORF Plasmid: tGFP
"NM_146148" in other vectors (4)
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CNY 4,376.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209163 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGTGGTTGCTTTCTTTGGCTTGTCTTTGGTGGCATGGCACCCTGGGGTAACTGCACAAGAGAAGG TGAACCAGCGGGTCACCCGAGCTGTGACCCCGCAAGCAGTGTCCTGTCAGTTGAGTGACTGGTACAAATG GACAGATTGTTTTCCATGTCAGGATAAAAAGTACCGATACCGGAGCCTCTTGCAGCCAAGCAAGTTTGGA GGAACCATCTGCAGTGGTGATATCTGGGATGAGGCCAGCTGTGACAGCCCCACACCTTGTCTAAGGCAAG CACAGTGTGGACAGGATTTCCAGTGTAGAGAGACAGGTCGCTGCCTGAAACGCCACCTGGTGTGTAACGG AGATAACGACTGCCTGGATGGCTCTGATGAGTCCGACTGTGAAGATGTCAGGGTCACTGAGGATGATTGC CATCAGTATGAACCAATCCCAGGATCAGAAAGGGCAGCCTTGGGGTACAACATCCTGACTCAAGAGGAAG CTCAGAGTGTTTACGATGCTAAGTATTATGGGGGCCAGTGTGAAACTGTGTACAATGGAGACTGGAGAAA GCTGCGGTACGACCCCACCTGTGAGCGCCTCTACTATGGGGAGGATGAGAAATACTTTCGGAAGCCCTAC AACTTCCTTAAGTACCACTTTGAAGCCCTGGCGGATACTTCAATCTCCTCAGAGTTTTATGATGATGCGA ATGATCTTTTTTTTCACATAAAGAATGGCAAGTCCCACTCAGCTGGTGTAACCGTTGGCGTAGCCCCAGT AAAAAGTCCTGTCTCAATAGAGGTGACTGGATCCGGGTCCAAAGCATCTTCATTCCTGAACAAGTTAAAC AAGTATAATGAGAAGAGATATGGCTTCATGAGAGTGTCTACAAAGATACAGACTGCACAGTTTAAGATGA GAAGGAACAATATTGTGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTCCCAGAGCAGTTCAACTA TGGCATGTACGCCAAGTTCATCAATGACTATGGCACCCACTACATCACCTCTGGAACCATGGGTGGCATT TATGAATATGTTATGGTGCTTGACAAAGAGAAAATGAAAACTGAAGGTACTACTGTGGATGAAGTCCAAA AATGCATTGGCGGTGGCATTGGCATAGGAATTAAGGATAGTACAATTGAAGGAGTAGGTATATCAGGAGA GTTTTGTGAAAACTCTGGAGATGGTGACCGTGACATAAGGAAGAAAATCACAGGAGTGGAGGACATCATT TCTCGGGTGCAAGGTGGAAGTTCTGTCTGGGGCAGTGTCTTGACACACAACAGCAGTGCCATTACATATC AGTCCTGGGGGAGATCGTTAAAGTATAACCCTGTTGTTATCGATTTTGAGATGCAGCCTATCTACCAGCT ACTGCGGCACACAAACTTGGGACCCCTGGAGACGAAACGTCAGAACCTGCGACGGGCTTTGGACCAGTAC CTGATGGAATTTAATGCCTGTCGTTGTGGGCCCTGCTTCAACAATGGGGAACCCATACTTGACGGCACCA ACTGCCGGTGTCAGTGTAGTATGGGCCGGCAGGGCCTGGCCTGTGAACGCACGGTGATAGAGGGGCTCAA AGACTTCAAAGCCGCTGGCCACTGGAGCTGCTGGAGTTCCTGGTCAGAGTGCAGAGGAGGTTCCCAGGAG AGGAGGAGACAGTGCAACAACCCACCACCCAAGAATGGAGGTACCCCATGCCTAGGGAGGAACCTGCAGA CCCAGGCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209163 protein sequence
Red=Cloning site Green=Tags(s) MFVVAFFGLSLVAWHPGVTAQEKVNQRVTRAVTPQAVSCQLSDWYKWTDCFPCQDKKYRYRSLLQPSKFG GTICSGDIWDEASCDSPTPCLRQAQCGQDFQCRETGRCLKRHLVCNGDNDCLDGSDESDCEDVRVTEDDC HQYEPIPGSERAALGYNILTQEEAQSVYDAKYYGGQCETVYNGDWRKLRYDPTCERLYYGEDEKYFRKPY NFLKYHFEALADTSISSEFYDDANDLFFHIKNGKSHSAGVTVGVAPVKSPVSIEVTGSGSKASSFLNKLN KYNEKRYGFMRVSTKIQTAQFKMRRNNIVLDEGMLQSLMELPEQFNYGMYAKFINDYGTHYITSGTMGGI YEYVMVLDKEKMKTEGTTVDEVQKCIGGGIGIGIKDSTIEGVGISGEFCENSGDGDRDIRKKITGVEDII SRVQGGSSVWGSVLTHNSSAITYQSWGRSLKYNPVVIDFEMQPIYQLLRHTNLGPLETKRQNLRRALDQY LMEFNACRCGPCFNNGEPILDGTNCRCQCSMGRQGLACERTVIEGLKDFKAAGHWSCWSSWSECRGGSQE RRRQCNNPPPKNGGTPCLGRNLQTQAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146148 |
| ORF Size | 1764 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146148.2, NP_666260.1 |
| RefSeq Size | 3636 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 230558 |
| UniProt ID | Q8K182 |
| MW | 66.1 kDa |
| Gene Summary | This gene encodes the alpha subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the alpha subunit, which associates with the beta and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the beta subunit. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219464 | C8a (untagged) - Mouse complement component 8, alpha polypeptide (C8a), (10ug) |
CNY 7,510.00 |
|
| MG209163 | C8a (tGFP-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 5,040.00 |
|
| MR209163L3 | Lenti ORF clone of C8a (Myc-DDK-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 6,460.00 |
|
| MR209163L4 | Lenti ORF clone of C8a (mGFP-tagged) - Mouse complement component 8, alpha polypeptide (C8a) |
CNY 6,460.00 |
