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4432405B04Rik (BC058375) Mouse Tagged ORF Clone
CAT＃: MR209082
- TrueORF®
4432405B04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178)
ORF Plasmid: tGFP
"BC058375" in other vectors (4)
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CNY 4,312.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4432405B04Rik; Tect2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209082 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTAGTTTCAGCCTCCCACAATGCATCCTGCTTAGCACATCTGCTCATCCAGGTGGAGATTTATC CCAACACATCTGTAACCCACAACGCATCAGAGAACATGACTGTCATTCCTAACCAAGTCTATCAGCCCCT GGGTCCCTGCCCGTGTGACTTAACAGCCAAGGCCTGTGACATTCAATGCTGCTGTGACCAGGACTGTCAG CCAGAAGTAAGAGAGCTGTTTGCACAGTCCTGCTTCTCCGGCGTGTTTGGAGGGCACGTCAGCCCTCCGT CCCATCATCACTGCGCTGTCAGCACTACTCACCAGACTCCTGACTGGTTTCCCCTCCTGTGTGTGCAGTC GCCACCCTCTACCTCGCCGTTCCTTGGTCACTTCTACCACGGTGCCACTTCCCCCAGACATTCCCCCGGC TTTGAAGCCCATTTGCACTTCGACCTAAGAGACTTCGCGGATGCCAGTTACAAGCAAGGAGATCCCATTA TGACCACGGAGGGGTATTTTACCATCCCCCAGGTGTCTCTAGCTGGGCAGTGTCTACAGGATGCTCCTGT GGCTTTCCTGAGGAATTTTGACAGTGTATGTACCATGGACCTGGAAGTGCACCAAGGACGAGATGAAATT GTCCTCGAGAACATGAAAATCCGCACAACAGGAGGTCCGGTCACACCAACAGTAACCTATGAGGAAGCCA TTGACCTGGACAAGTTCATCACCAGCCCGGACACAGTTTTAAGTGTCGGATCGGCCCCCAGAAACGTGAA TGTGGAAGAACATTATGTCTTCAGATGGCAAAATAATTCCATCAGCGGCCTAGACATCACAGTCATCAGG GCAGAAATCAGTGCCCAGCAGAGAGGAATGATGACCCAGAGATTCACAGTGAAGTTTTTAAGCCATCACA GTGGTGGTGAGAAGGAATTTTCTGGAAATCCAGGTTACCAGCTTGGCAAGCCGGTCCGAGCCCTGCATAC CGCTGGGATGAACGTCAGCACACTGCACCTCTGGCAGCCAGCTGGAAGGGGCCTGTGCACAGCAGCCGCT CTCAGACCCATTTTGTTTGGAGAAAATGCCTTCTCTGGCTGCCTGCTGGAAGTTGGGATTAAGGAGAACT GTACCCAGCTCAGAGAGAATGTCCTTCAGAGACTGGATTTGCTGACACAAGCAACGCACGTGGCGAGGAG AGGCAACTCGGACTACAGTGATCTGAGTGACGGCTGGCTGGAGATCATACGTGCCGAAGCCCCTGATACA GGTGCCGACCTGCCCTTGAGCAGCGTGAATGGCGTGTGCCCAGAAGTTCCAGCCCGGCTCTCCATCCGCA TTCTCACTGCTGAAGCTGGCTCTGTGGAAGGGGTGGCTCAGCGGGAGATCCTGGCTGTGGAGACAAGGTT TTCCACAGTGACCTGGCAGTACCAGTGTGGGCTCACTTGTGAGGACAAGGCCGACCTTCTCCCTCTCAGT GCCTCTGTTGAGTTCATTAATGTCCCTGCGCAGATGCCCCACCCCCCTACGAGATTCCAGATCAACTTCA CAGAGTACGACTGTACCCGGAATGAGCTGTGTTGGCCCCAACTTCTGTACCCTCTGACCCAGTATTATCA AGGGGAGCCCCAGTCTCAGTGTGTGGCCAAAGGCCTGATGCTGCTGTCACTCCTCATGCTGGCCATCCTC CTCAGGCACCCCTGGGTCAGGATGTGCAAAGCCCGGGACTCAGCTGCCATCTACCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209082 protein sequence
Red=Cloning site Green=Tags(s) MLLVSASHNASCLAHLLIQVEIYPNTSVTHNASENMTVIPNQVYQPLGPCPCDLTAKACDIQCCCDQDCQ PEVRELFAQSCFSGVFGGHVSPPSHHHCAVSTTHQTPDWFPLLCVQSPPSTSPFLGHFYHGATSPRHSPG FEAHLHFDLRDFADASYKQGDPIMTTEGYFTIPQVSLAGQCLQDAPVAFLRNFDSVCTMDLEVHQGRDEI VLENMKIRTTGGPVTPTVTYEEAIDLDKFITSPDTVLSVGSAPRNVNVEEHYVFRWQNNSISGLDITVIR AEISAQQRGMMTQRFTVKFLSHHSGGEKEFSGNPGYQLGKPVRALHTAGMNVSTLHLWQPAGRGLCTAAA LRPILFGENAFSGCLLEVGIKENCTQLRENVLQRLDLLTQATHVARRGNSDYSDLSDGWLEIIRAEAPDT GADLPLSSVNGVCPEVPARLSIRILTAEAGSVEGVAQREILAVETRFSTVTWQYQCGLTCEDKADLLPLS ASVEFINVPAQMPHPPTRFQINFTEYDCTRNELCWPQLLYPLTQYYQGEPQSQCVAKGLMLLSLLMLAIL LRHPWVRMCKARDSAAIYH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058375 |
| ORF Size | 1737 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC058375, AAH58375 |
| RefSeq Size | 2339 bp |
| RefSeq ORF | 1739 bp |
| Locus ID | 67978 |
| MW | 63.9 kDa |
| Gene Summary | Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Required for hedgehog signaling transduction.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217847 | 4432405B04Rik (untagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178), (10ug) |
CNY 6,940.00 |
|
| MG209082 | 4432405B04Rik (tGFP-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178) |
CNY 3,710.00 |
|
| MR209082L3 | Lenti ORF clone of 4432405B04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178) |
CNY 5,230.00 |
|
| MR209082L4 | Lenti ORF clone of 4432405B04Rik (mGFP-tagged) - Mouse RIKEN cDNA 4432405B04 gene (cDNA clone MGC:66869 IMAGE:6828178) |
CNY 5,230.00 |
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