Scg2 (BC048249) Mouse Tagged ORF Clone

CAT#: MR209051

  • TrueORF®

Scg2 (Myc-DDK-tagged) - Mouse secretogranin II (cDNA clone MGC:54765 IMAGE:6493827)

ORF Plasmid: DDK tGFP



  "BC048249" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,296.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Chgc; SgII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209051 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGAGCTAAGGCGTACCGACTTGGAGCAGTTCTGCTTCTTATCCACTTAATTTTCCTCATCTCTG
GAGCCGAAGCAGCTTCCTTCCAGCGAAACCAGCTGCTTCAGAAAGAACCAGACCTCAGATTGGAGAATGT
CCAAAAGTTTCCTAGTCCAGAAATGATCAGGGCTTTGGAGTACATAGAAAAGCTCAGGCAGCAAGCTCAC
AGAGAAGAAAGCAGCCCAGACTACAATCCCTACCAAGGCGTCTCTGTTCCTCTTCAACTCAAAGAAAACG
GAGAAGAAAGCCACTTGGCAGAGAGCTCAAGGGATGCACTGAGTGAAGACGAGTGGATGCGGATAATACT
CGAGGCTCTGAGGCAGGCTGAAAATGAGCCGCCATCTGCCCCCAAAGAGAACAAGCCCTATGCCTTGAAT
CTGGAGAAGAACTTCCCAGTGGACACGCCTGATGACTATGAGACTCAACAGTGGCCTGAGAGGAAACTCA
AGCACATGCGGTTCCCTCTCATGTATGAAGAGAATTCCAGAGAAAACCCCTTCAAACGCACAAATGAAAT
AGTCGAGGAACAATACACACCCCAAAGTCTTGCTACCCTGGAGTCTGTGTTCCAAGAGCTTGGGAAACTG
ACAGGGCCAAGCAACCAGAAGCGTGAGAGGGTTGACGAGGAACAAAAGCTGTACACAGATGATGAAGACG
ACGTGTACAAGACCAACAACATTGCCTATGAAGATGTCGTGGGGGGAGAAGACTGGAGCCCCATAGAGGA
GAAAATAGAGACTCAAACCCAGGAAGAGGTGAGAGACAGCAAAGAGAACACAGAAAAAAATGAACAAATC
AATGAAGAGATGAAACGTTCAGGGCAGTTGGGGCTCCCAGATGAAGAAAACCGGAGAGAGAGTAAAGACC
AACTCTCAGAGGATGCCTCCAAAGTTATCACCTACCTGAGAAGGAATTTGCAGATACCCCCTGAAGATTT
AATTGAGATGCTCAAAGCTGGAGAGAAGCCAAATGGGTTGGTGGAGCCAGAGCAGGATCTGGAGCTTGCT
GTTGACCTAGATGACATCCCAGAGGCTGACCTAGACCGTCCAGACATGTTTCAAAGTAAGATGCTCTCCA
AGGGTGGGTATCCCAAGGCACCTGGTCGTGGTATGGTAGAGGCCTTGCCTGATGGGCTGAGTGTCGAGGA
CATTTTAAATGTTTTAGGGATGGAGAATGTAGTAAATCAGAAGTCCCCATATTTTCCCAACCAATATAGC
CAAGACAAGGCTCTGATGAGGCTCCCTTATGGTCCTGGGAAATCTAGAGCCAACCAGATTCCCAAAGTAG
CCTGGATCCCTGATGTTGAAAGCAGACAAGCACCTTATGAAAATCTGAATGACCAAGAATTGGGAGAGTA
CTTAGCCAGGATGCTAGTTAAGTACCCTGAGCTCCTGAATACCAACCAGCTGAAGAGAGTGCCCAGTCCA
GTCTCCTCAGAGGATGACCTCCAAGAAGAAGAGCAGCTCGAGCAGGCCATCAAGGAACATCTGGGGCCAG
GAAGCTCCCAGGAAATGGAGAGACTGGCCAAGGTGAGCAAAAGGATCCCCGTAGGATCCCTGAAGAATGA
GGACACCCCAAACAGACAGTACCTGGATGAAGATATGCTCCTGAAAGTGCTGGAGTACCTCAACCAAGAG
CAGGCAGAGCAGGGGAGGGAGCATCTTGCCAAGCGGGCCATGGAAAACATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209051 protein sequence
Red=Cloning site Green=Tags(s)

MAGAKAYRLGAVLLLIHLIFLISGAEAASFQRNQLLQKEPDLRLENVQKFPSPEMIRALEYIEKLRQQAH
REESSPDYNPYQGVSVPLQLKENGEESHLAESSRDALSEDEWMRIILEALRQAENEPPSAPKENKPYALN
LEKNFPVDTPDDYETQQWPERKLKHMRFPLMYEENSRENPFKRTNEIVEEQYTPQSLATLESVFQELGKL
TGPSNQKRERVDEEQKLYTDDEDDVYKTNNIAYEDVVGGEDWSPIEEKIETQTQEEVRDSKENTEKNEQI
NEEMKRSGQLGLPDEENRRESKDQLSEDASKVITYLRRNLQIPPEDLIEMLKAGEKPNGLVEPEQDLELA
VDLDDIPEADLDRPDMFQSKMLSKGGYPKAPGRGMVEALPDGLSVEDILNVLGMENVVNQKSPYFPNQYS
QDKALMRLPYGPGKSRANQIPKVAWIPDVESRQAPYENLNDQELGEYLARMLVKYPELLNTNQLKRVPSP
VSSEDDLQEEEQLEQAIKEHLGPGSSQEMERLAKVSKRIPVGSLKNEDTPNRQYLDEDMLLKVLEYLNQE
QAEQGREHLAKRAMENM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC048249
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC048249, AAH48249
RefSeq Size 2211 bp
RefSeq ORF 1733 bp
Locus ID 20254
MW 66.4 kDa
Gene Summary Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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