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Fut8 (NM_016893) Mouse Tagged ORF Clone
CAT＃: MR209034
- TrueORF®
Fut8 (Myc-DDK-tagged) - Mouse fucosyltransferase 8 (Fut8). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_016893" in other vectors (4)
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CNY 4,280.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209034 representing NM_016893, codon optimized.
Due to the complexity of NM_016893, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACCTTGTTATTTT ATATAGGTGGTCATTTGGTTCGAGATAATGACCACCCTGATCACTCCAGCAGAGAACTCTCCAAGATTCT TGCAAAGCTTGAACGCTTAAAACAGCAAAATGAAGACTTGAGGCGAATGGCTGAGTCTCTCCGAATACCA GAAGGCCCCATTGACCAGGGGACAGCTACAGGAAGAGTCCGTGTTTTAGAAGAACAGCTTGTTAAGGCCA AAGAACAGATTGAAAATTACAAGAAACAAGCTAGAAATGGTCTGGGGAAGGATCATGAAATCTTAAGAAG GAGGATTGAAAATGGAGCTAAAGAGCTCTGGTTTTTTCTACAAAGCGAACTGAAGAAATTAAAGCATTTA GAAGGAAATGAACTCCAAAGACATGCAGATGAAATTCTTTTGGATTTAGGACACCATGAAAGGTCTATCA TGACAGATCTATACTACCTCAGTCAAACAGATGGAGCAGGGGATTGGCGTGAAAAAGAGGCCAAAGATCT GACAGAGCTGGTCCAGCGGAGAATAACATATCTCCAGAATCCTAAGGACTGCAGCAAAGCCAGGAAGCTG GTGTGTAACATCAATAAAGGCTGTGGCTATGGTTGTCAACTCCATCACGTGGTCTACTGTTTCATGATTG CTTATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGAC TGTATTCAGGCCTGTGAGTGAGACATGTACCGACAGATCTGGACTGAGCACAGGGCATTGGTCCGGAGAG GTTAACGACAAGAACATTCAGGTTGTTGAGCTCCCTATTGTGGATTCCCTTCATCCTAGACCGCCATATC TCCCACTGGCCGTGCCTGAGGACCTCGCTGACCGCCTGCTGCGGGTTCATGGGGACCCGGCCGTCTGGTG GGTGTCTCAGTTTGTGAAGTATCTTATCAGACCGCAGCCCTGGCTGGAAAAGGAGATAGAGGAGGCCACT AAAAAGCTGGGATTCAAACATCCCGTGATTGGAGTACATGTGCGAAGAACTGATAAAGTGGGCACTGAAG CGGCATTTCATCCAATCGAAGAATACATGGTACACGTGGAGGAGCATTTTCAGCTTCTGGCGCGAAGGAT GCAGGTTGACAAGAAGCGAGTTTACCTGGCCACTGATGACCCAACGCTGCTGAAAGAGGCTAAGACTAAG TACTCCAATTATGAGTTTATCAGTGACAATTCCATATCTTGGAGCGCCGGCCTGCACAACCGCTATACAG AGAATAGCCTGCGCGGTGTCATCTTGGATATCCATTTCCTTTCTCAGGCCGACTTCCTCGTCTGTACTTT CTCTTCACAGGTATGCAGAGTAGCCTACGAAATCATGCAGACACTCCACCCAGATGCCTCCGCAAATTTT CATAGCCTTGATGATATCTACTACTTTGGTGGGCAGAATGCCCATAACCAGATCGCAGTTTACCCTCATA AGCCCCGGACTGAGGAAGAGATTCCCATGGAACCAGGGGACATTATCGGGGTGGCCGGTAACCATTGGGA TGGCTATAGCAAGGGGATTAATCGGAAGCTGGGTAAGACCGGTCTTTACCCTTCCTACAAGGTTCGGGAA AAGATCGAAACAGTGAAATACCCAACATACCCAGAGGCGGAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209034 representing NM_016893
Red=Cloning site Green=Tags(s) MRAWTGSWRWIMLILFAWGTLLFYIGGHLVRDNDHPDHSSRELSKILAKLERLKQQNEDLRRMAESLRIP EGPIDQGTATGRVRVLEEQLVKAKEQIENYKKQARNGLGKDHEILRRRIENGAKELWFFLQSELKKLKHL EGNELQRHADEILLDLGHHERSIMTDLYYLSQTDGAGDWREKEAKDLTELVQRRITYLQNPKDCSKARKL VCNINKGCGYGCQLHHVVYCFMIAYGTQRTLILESQNWRYATGGWETVFRPVSETCTDRSGLSTGHWSGE VNDKNIQVVELPIVDSLHPRPPYLPLAVPEDLADRLLRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEAT KKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPTLLKEAKTK YSNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANF HSLDDIYYFGGQNAHNQIAVYPHKPRTEEEIPMEPGDIIGVAGNHWDGYSKGINRKLGKTGLYPSYKVRE KIETVKYPTYPEAEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016893 |
| ORF Size | 1725 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016893.1, NM_016893.2, NM_016893.3, NM_016893.4, NM_016893.5, NP_058589.2 |
| RefSeq Size | 2923 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 53618 |
| UniProt ID | Q9WTS2 |
| MW | 66.6 kDa |
| Gene Summary | Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200696 | Fut8 (untagged) - Mouse fucosyltransferase 8 (Fut8), (10ug) |
CNY 4,288.00 |
|
| MG209034 | Fut8 (tGFP-tagged) - Mouse fucosyltransferase 8 (Fut8). Note: ORF is codon optimized |
CNY 5,040.00 |
|
| MR209034L3 | Lenti ORF clone of Fut8 (Myc-DDK-tagged) - Mouse fucosyltransferase 8 (Fut8). Note: ORF is codon optimized |
CNY 6,680.00 |
|
| MR209034L4 | Lenti ORF clone of Fut8 (mGFP-tagged) - Mouse fucosyltransferase 8 (Fut8). Note: ORF is codon optimized |
CNY 6,460.00 |
