Tox3 (NM_172913) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	500-9; BC052044; C230068E13; CAGF9; Tnrc9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR209033 representing NM_172913 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGAGGTTCTACCCCGCGGCGGCCGGGGACCCAGCCGGCCTGGACTTCGCGCAGTGCCTGGGGT ACTACGGCTACAGCAAGTTGGGAAATAATAACTACATGAACATGGCCGAGGCAAACAACGCCTTCTTTGC TGCCAGTGAGCAGACATTCCACACGCCAAGCCTTGGGGACGAAGAGTTTGAAATTCCACCGATCACGCCT CCTCCAGAGTCAGACCCCACCCTGGGTATGCCTGATGCACTGCTACCCTTTCAGACTCTCAGCGATCCGT TGCCTTCCCAGGGAACTGAGTTCACACCCCAGTTTCCCCCTCAAAGCCTGGATCTCCCTTCCATCACAAT CTCAAGGAATCTGGTGGAGCAAGATGGCGTGCTTCATAGCAATGGGCTGCACATGGATCAGAGCCACACA CAAGTGTCACAGTACCGCCAGGATCCCTCTTTGGTCATGAGGTCAATTGTCCATATGACGGATGGTGCTC GATCTGGGATCATGCCTCCTGCCCAGCTGACCACCATCAACCAGTCTCAGCTCAGCGCACAGCTGGGCTT GAATTTGGGAGGAGCCAATGTGTCCCACACATCTCCTTCACCTCCAGCAAGCAAATCAGCCACTCCCTCT CCTTCCAGCTCTATCAACGAAGAGGATGCTGATGATGCAAACAGAGCCATTGGAGAGAAAAGAACAGCCC CAGATTCTGGCAAGAAGCCCAAGACTCCAAAGAAAAAGAAAAAGAAAGATCCCAACGAGCCTCAGAAGCC AGTGTCAGCATATGCCCTGTTTTTCAGAGATACACAGGCTGCAATTAAGGGTCAAAACCCCAACGCAACC TTCGGAGAAGTCTCGAAAATCGTAGCATCTATGTGGGACAGCCTTGGGGAGGAGCAAAAGCAGGTATATA AAAGGAAAACAGAAGCTGCCAAGAAAGAATACTTGAAGGCCCTGGCTGCCTACCGGGCTAGCCTCGTTTC CAAGGCTGCTGCTGAATCTGCAGAAGCCCAGACCATCCGTTCTGTCCAGCAGACTCTGGCATCAACCAAC CTGACATCCTCCCTCCTCCTGAACACATCACTGTCTCAACATGGGACAGTCCCAGCCTCACCTCAGACTC TTCCACAGTCACTCCCTAGGTCAATTGCCCCCAAACCCTTAACCATGAGACTACCCATGAGCCAGATCGT CACATCAGTCACCATTGCAGCCAACATGCCCTCGAACATTGGGGCTCCACTGATAAGTTCCATGGGGACG ACCATGGTTGGTTCAGCAACCTCCACCCAGGTGAGCCCTTCGGTGCAAACCCAGCAGCATCAGATGCAGT TGCAGCAGCAACAGCAGCAGCAGCAGCAGATGCAGCAGATGCAGCAGCAGCAGTTACAGCAGCACCAAAT GCATCAGCAGATCCAGCAGCAGATGCAGCAGCAGCATTTTCAGCATCACATGCAGCAGCACCTGCAGCAG CAGCAACAGCAGCATCTCCAGCAGCAGCTCAGCCAACAGCAGCTGCAGCAGCAGTTGCAGCAGCATCTCC AGCTGCAGCAACTGCAGCACATGCAGCACCAGTCTCAGCCTTCTCCCCGGCAGCACTCGCCCGTCACCTC ACAGATCACGTCCCCCATCCCCGCCATTGGCAGCCCCCAGCCAGCCTCTCAGCAGCACCAGCCTCAAATC CAGTCGCAGACTCAGACTCAAGTGTTACCGCAGGTCAGTATTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR209033 representing NM_172913 Red=Cloning site Green=Tags(s) MDVRFYPAAAGDPAGLDFAQCLGYYGYSKLGNNNYMNMAEANNAFFAASEQTFHTPSLGDEEFEIPPITP PPESDPTLGMPDALLPFQTLSDPLPSQGTEFTPQFPPQSLDLPSITISRNLVEQDGVLHSNGLHMDQSHT QVSQYRQDPSLVMRSIVHMTDGARSGIMPPAQLTTINQSQLSAQLGLNLGGANVSHTSPSPPASKSATPS PSSSINEEDADDANRAIGEKRTAPDSGKKPKTPKKKKKKDPNEPQKPVSAYALFFRDTQAAIKGQNPNAT FGEVSKIVASMWDSLGEEQKQVYKRKTEAAKKEYLKALAAYRASLVSKAAAESAEAQTIRSVQQTLASTN LTSSLLLNTSLSQHGTVPASPQTLPQSLPRSIAPKPLTMRLPMSQIVTSVTIAANMPSNIGAPLISSMGT TMVGSATSTQVSPSVQTQQHQMQLQQQQQQQQQMQQMQQQQLQQHQMHQQIQQQMQQQHFQHHMQQHLQQ QQQQHLQQQLSQQQLQQQLQQHLQLQQLQHMQHQSQPSPRQHSPVTSQITSPIPAIGSPQPASQQHQPQI QSQTQTQVLPQVSIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_172913
ORF Size	1725 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_172913.3, NP_766501.2
RefSeq Size	3212 bp
RefSeq ORF	1728 bp
Locus ID	244579
UniProt ID	Q80W03
MW	63.6 kDa
Gene Summary	Transcriptional coactivator of the p300/CBP-mediated transcription complex. Activates transactivation through cAMP response element (CRE) sites. Protects against cell death by inducing antiapoptotic and repressing pro-apoptotic transcripts. Stimulates transcription from the estrogen-responsive or BCL-2 promoters. Required for depolarization-induced transcription activation of the C-FOS promoter in neurons. Associates with chromatin to the estrogen-responsive C3 promoter region (By similarity).[UniProtKB/Swiss-Prot Function]

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