Zfp354a (BC087540) Mouse Tagged ORF Clone

CAT#: MR208995

  • TrueORF®

Zfp354a (Myc-DDK-tagged) - Mouse zinc finger protein 354A (cDNA clone MGC:91222 IMAGE:4239789)

ORF Plasmid: DDK tGFP



  "BC087540" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 10,384.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Zfp354a Antibody - C-terminal region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AW488485; kid1; Tcf17; Znf354a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208995 representing BC087540
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

GTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCCATGATCCCCATGCAACGGCGGGAGGACTGCA
AAGGGCTGGTCTTCCTGGGAGTCCTCCTCGGGATGCTGTGGAAAGCTGGATCCACCCAGATACGCTATTC
GGTTCCCGAGGAGCTGGAGAAAGGATCTAGGGTGGGCAACATCTCCAAGGATCTGGGGCTGGAGCCCCGC
GAGCTGGAGAAGCGAGGGGTCCGCATCGTCTCCAGAGGTAGGGCTCAACTTTTCGCTCTAAACCCTCGAA
GCGGCAGCCTGGTCACCGCGGGCAGGATAGACCGGGAGGAGCTGTGTATGGGGTCCATCAAGTGTCAGTT
AAATCTAGAGATTCTGATGGAAGATATAGGGAAAATATACGGAGTAGAAATAGAAGTGAGGGACATTAAC
GACAACGCTCCCTACTTCCGTGAAAGTGAATTAGAAATAAAAATGAGCGAAAACGCCGCCGCTGGGATGC
GGTTCCCTCTTCCCCACGCTTGGGATCCAGATATCGGGAAGAACTCGCTTCAGAGCTACCAGCTCAGCCC
GAATGCTCACTTCTCACTGGAGGTGCAGAACGGAGCTGATGGTAACAAGTACCCAGAATTGGTGCTGGAG
AGCTCGCTGGATCGGGAGGAAAAGGCCGCTCATCTCCTAGTCCTCACCGCTTCCGACGCGGGAGACCCGG
TTCGCACTGGCACAGCGCGCATCCGCGTGATGGTTGTGGATGCGAACGACAACGCACCTGCGTTTGCTCG
GTCCGAGTACCGCGTGAGCGTGCGGGAGAACGTGGCTGTGGGCACTCAGCTGCTGCTGGTCAACGCCACC
GACCCAGACGAAGGCGCCAATGCGGAAGTGATCTATTCCTTCCGGTACGTGGACGACAAGGCAGCGAAAG
TTTTCAAGCTGGACAGTAACTTGGGGACAATATCAACAATAGGGGAGCTGAATCATGAAGAATCGGGGTT
CTACGAGATGGAGGTGCAAGCTACAGATAACGCGGGATATTCTGCACGGGCCAAGGTCCTGGTCACAGTT
CTGGACGTGAACGACAATGCCCCTGAAGTAGCCATCACGTCCCTCACCAACTCTGTCCCAGAAAACTCTC
CCCAAGGGACATTAATAGCACTTTTAAACGTAAATGATCAAGATTCTGGGGAAAATGGACAGGTAATCTG
TTCCATCCAAGAGAATCTGCCCTTTAAGTTAGAAAAGTCTTACGGAAACTATTATAGATTAGTCACAGAT
GCAGTCCTGGACCGAGAAGAGGTTCCTAGTTACAACATCACAATGACCGCCACTGACAGGGGAAGTCCGC
CCCTGACAACAGAAACTCACCTCGCACTGGACATAGCAGACACGAACGATAACTCGCCCGTTTTCCTTCA
GGCCTCATACTGGGCCTACATCCCAGAGAATAACCCTAGAGGGGCCTCTATCGCATCCGTGACCGCCCAC
GACCCCGACAGTGACAAAAATGCCCAAGTCACTTACTCCCTAGCTGAGGACACCCACCAGGGCGTGCCCC
TTTCCTCTTACGTTTCCATCAACTCGGACACTGGTGTCCTGTACGCACTGCATTCCTTTGACTACGAGCA
GTTCCCAGACCTACAACTGCAAGTGATAGCGCGTGACAGCGGGGACCCGCCACTCAGCAGCAACGTGTCA
CTGAGCTTGTTCGTGCTGGATCAGAATGACAACGTCCCCGAAATCTTGTACCCCACTCTCCCTACCGACG
GTTCTACTGGAGTGGAGCTAGCACCCCGCTCAGCAGAGCCTGGCTATCTGGTGACAAAGGTGGTGGCAGT
GGACAGAGACTCAGGACAGAACGCCTGGCTGTCCTACCGTCTGCTAAAGGCCAGCGAGCCAGGGCTCTTC
TCCGTGGGGCTGCACACGGGCGAGATAAGCACGGCTCGGGCCCTGATGGACAGAGATGCTCTCAAACAGA
ACCTCGTAGTGTCTGTGCAGGACCACGGCCAGCCTCCTCTCTCTGCCACTGTCACGCTCACTGTGGCGAT
CGCCGACAGCATCCCCGACGTCCTGGCAGATCTAGACAATCTAGAATCTCCTTCCACCTCCGAAACTTCA
GGGCTCACTCTCTATCTGGTGGTGGCCGTGGCGGCGGTCTCCACCGTCTTCCTGGTCTTTGTTCTGGTGC
TGCTGGCGCTCAGGCTGCGGCGCTGGCACTCACTGCGCCTGCTCCGGGCTGGGGGACCCGGATTGGCTGA
CGTTGCAGCCTCTCACTTTGTGGGCGTAGACGGAGTGCAAGCTTTCTTGCAGACTTATTCGCACGAGGTC
TCGCTCACTGCGGGCTCCCGAAAGAGTCACTTGATTTTCCCGCAGCCCAACTATGCAGACACGCTCATCA
GCCAGGAGAGCTGCGAGAAAACCGAGCCTCTTTTACCATCGGGTGATTCGGTTTTTTCTAAAGACAATCA
TGCATTAAATCAGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCGGCACGAGC
GGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCTGCAAGCCA
TGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCTGGCACCATGGG
TCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGAACGTGTACATC
CCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAGGCTCCAGCAGGTGGTAATG
GCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208995 representing BC087540
Red=Cloning site Green=Tags(s)

VPRRSAAAIAGAPMIPMQRREDCKGLVFLGVLLGMLWKAGSTQIRYSVPEELEKGSRVGNISKDLGLEPR
ELEKRGVRIVSRGRAQLFALNPRSGSLVTAGRIDREELCMGSIKCQLNLEILMEDIGKIYGVEIEVRDIN
DNAPYFRESELEIKMSENAAAGMRFPLPHAWDPDIGKNSLQSYQLSPNAHFSLEVQNGADGNKYPELVLE
SSLDREEKAAHLLVLTASDAGDPVRTGTARIRVMVVDANDNAPAFARSEYRVSVRENVAVGTQLLLVNAT
DPDEGANAEVIYSFRYVDDKAAKVFKLDSNLGTISTIGELNHEESGFYEMEVQATDNAGYSARAKVLVTV
LDVNDNAPEVAITSLTNSVPENSPQGTLIALLNVNDQDSGENGQVICSIQENLPFKLEKSYGNYYRLVTD
AVLDREEVPSYNITMTATDRGSPPLTTETHLALDIADTNDNSPVFLQASYWAYIPENNPRGASIASVTAH
DPDSDKNAQVTYSLAEDTHQGVPLSSYVSINSDTGVLYALHSFDYEQFPDLQLQVIARDSGDPPLSSNVS
LSLFVLDQNDNVPEILYPTLPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLF
SVGLHTGEISTARALMDRDALKQNLVVSVQDHGQPPLSATVTLTVAIADSIPDVLADLDNLESPSTSETS
GLTLYLVVAVAAVSTVFLVFVLVLLALRLRRWHSLRLLRAGGPGLADVAASHFVGVDGVQAFLQTYSHEV
SLTAGSRKSHLIFPQPNYADTLISQESCEKTEPLLPSGDSVFSKDNHALNQQAPPNTDWRFSQAQRPGTS
GSQNGDETGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYI
PGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC087540
ORF Size 2835 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC087540.1
RefSeq Size 4180 bp
RefSeq ORF 1715 bp
Locus ID 21408
MW 153.2 kDa
Gene Summary It may play a role in renal development and may also be involved in the repair of the kidney after ischemia-reperfusion or folic acid administration.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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