Chst15 (NM_029935) Mouse Tagged ORF Clone
CAT#: MR208889
- TrueORF®
Chst15 (Myc-DDK-tagged) - Mouse carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (Chst15)
ORF Plasmid: tGFP
"NM_029935" in other vectors (4)
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CNY 4,184.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4631426J05Rik; BRAG; GalNAcS-6ST; MAd5; mKIAA0598 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208889 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCATTGCATTAATTGCTGCGTCCAGCTGTTCCCCGAAGACACACACAAACAGCAAGTTGCCTGCC AAGGAGGCCCCCATCACAGTCATCAGGCGTGCCCCACTTGCAAAGGAGAAAACAAAATTCTGTTTCGTGT GGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTGAGGACTGAGGGCAATGAAAACTGGGGTGGG TTTTTGCGCTTCAGGAAGGGGAAGCGATGTAGCCTGGTCTTTGGATTGATAATAATGACCTTGGTGATGG CTTCTTACATCCTTTCCGGGGCTCATCAGGAACTTCTGATTTCCTCCCCTTTCCATTATGGGGGCTTTCC CAGCAACCCCAGCGTGATGGATGGTGAGAACCCCAGTGATGTGAAAGAGCATCACTACCAGCCTTCTGTA AATAACATCTCCTATGTAAAGGACTATCCGAGCATTAAACTGATTATCGACAGCATTGCTGCCAGGATTG AGTTCACAACCAGGCAGCTCCCGGACTTACAAGATCTCAAGAGACAAGAGTTGCATATGTTTTCTGTAAT CCCCAGCAAATTCCTCCCGACTAGCAAGAGCCCTTGTTGGTATGAGGAGTTCTCGGGCAGGAACACCACT GACCCCTACCTGACCAATTCCTACGTGCTCTACTCCAAGCGGTTCCGCTCTACCTTTGACGCTCTGCGCA AGGTTTTCTGGGGCCACCTGTCCCACGTGCAGGGCAAGCACTTCCGCCTGCGCTGCCTGCCCCACTTCTA CATCATTGGGCAGCCCAAGTGTGGAACCACTGACCTGTACGACCGCCTCCGGCTGCATCCAGAAGTGAAA TTCTCAGCCATCAAGGAGCCGCACTGGTGGACCCGGAAGCGCTTTGGAATTGTCCGCCTGCGGGACGGAC TACGAGACCGCTACCCTGTGGAAGATTACCTGGACCTCTTTGACTTGGCTGCACACCAGATCCATCAAGG ACTGCAGGCTGCCTCTGCAGAGCAGCCCAGCAAGATGAATAAGATCATTATTGGAGAGGCCAGCGCCTCT ACAATGTGGGATAACAATGCCTGGACCTTCTTCTATGACAACAGCACAGACGGCGAGCCACCATTCCTGA CCCAAGACTTCATCCATGCCTTTCAACCAGAAGCCAAGCTCATTGTTATGCTCAGGGATCCTGTGGAGAG GTTGTACTCAGACTATCTCTACTTCGCAAGTTCCAATAAATCTGCAGATGACTTCCACGAGAAAGTGACG GAGGCTCTGCAGCTGTTTGAAAATTGCATGCTGGATTATTCACTGCGCGCCTGCGTCTACAACAACACCC TGAACAATGCCATGCCCGTGAGGCTCCAGGTTGGCCTGTATGCTGTGTACCTCCTGGATTGGCTGACTGT CTTCAGTAAGGAGCAGTTCCTCATTCTCCGTCTGGAAGACCACGCATCCAATGTCAAGTATACCATGCAC AAGGTCTTCCAGTTTCTAAACCTGGGGCCTCTGAGTGAGAAGCAGGAAGCTCTGATGACCAAGAGCCCTG CATCCAACACACGGCGTCCTGAGGATCGTAGTCTGGGACCCATGTGGCCCATCACCCAGAAGATTCTGCG TGAATTCTATGGGCCTTTCAACACAAGGCTGGCACAGGTCTTGGATGACGAAGCATTTGCCTGGAAGACA ACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208889 protein sequence
Red=Cloning site Green=Tags(s) MRHCINCCVQLFPEDTHKQQVACQGGPHHSHQACPTCKGENKILFRVDSKQMNLLAVLEVRTEGNENWGG FLRFRKGKRCSLVFGLIIMTLVMASYILSGAHQELLISSPFHYGGFPSNPSVMDGENPSDVKEHHYQPSV NNISYVKDYPSIKLIIDSIAARIEFTTRQLPDLQDLKRQELHMFSVIPSKFLPTSKSPCWYEEFSGRNTT DPYLTNSYVLYSKRFRSTFDALRKVFWGHLSHVQGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVK FSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQAASAEQPSKMNKIIIGEASAS TMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPEAKLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVT EALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLTVFSKEQFLILRLEDHASNVKYTMH KVFQFLNLGPLSEKQEALMTKSPASNTRRPEDRSLGPMWPITQKILREFYGPFNTRLAQVLDDEAFAWKT T TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_029935 |
ORF Size | 1686 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_029935.6 |
RefSeq Size | 4265 bp |
RefSeq ORF | 1686 bp |
Locus ID | 77590 |
UniProt ID | Q91XQ5 |
MW | 65 kDa |
Gene Summary | Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205498 | Chst15 (untagged) - Mouse carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (Chst15), (10ug) |
CNY 4,184.00 |
|
MG208889 | Chst15 (tGFP-tagged) - Mouse RIKEN cDNA 4631426J05 gene (4631426J05Rik) |
CNY 5,040.00 |
|
MR208889L3 | Lenti ORF clone of Chst15 (Myc-DDK-tagged) - Mouse carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (Chst15) |
CNY 6,460.00 |
|
MR208889L4 | Lenti ORF clone of Chst15 (mGFP-tagged) - Mouse carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 (Chst15) |
CNY 6,460.00 |