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Ythdf1 (NM_173761) Mouse Tagged ORF Clone
CAT＃: MR208856
- TrueORF®
Ythdf1 (Myc-DDK-tagged) - Mouse YTH domain family 1 (Ythdf1)
ORF Plasmid: tGFP
"NM_173761" in other vectors (4)
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CNY 6,256.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2210410K23Rik; 8030473O16 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208856 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCACCAGCGTGGACCCCCAGAGAACAAAAGGACAAGATAATAAAGTACAAAATGGTTCTTTGC ATCAGAAGGATGCAGTTCATGACAATGACTTTGAGCCCTACCTTTCTGGACAGTCCAATCCGAGTAACAG TTACCCCTCGATGAGTGATCCTTACCTGTCCAGTTACTATCCACCATCCATTGGATTTCCTTACTCCCTC AGCGAGGCACCATGGTCCACTGCAGGGGACCCTCCCATCCCGTATCTCACTACCTATGGACAACTTAGTA ATGGAGACCATCACTTCATGCATGATGCTGTTTTTGGGCAGCCTGGGGGTCTGGGGAACAACATTTACCA GCACAGGTTTAATTTTTTCCCTGAAAACCCTGCATTCTCAGCATGGGGGACAAGTGGTTCTCAGGGGCAG CAGACTCAGAGCTCAGCCTATGGGAGCAGTTACACTTACCCACCTAGCTCCCTTGGTGGCACAGTTGTTG ATGGGCAGACAGGTTTTCACAGTGACTCCCTCAACAAGGCCCCTGGGATGAACAGTCTGGAGCAGGGCAT GGTTGGCCTGAAGATTGGGGATGTTACCACCTCTGCAGTTAAGACGGTGGGTTCAGTTGTCAACAGTGTG GCACTGACTGGTGTCCTTTCTGGCAATGGTGGGACAAATGTAAACATGCCAGTTTCAAAACCAACTTCAT GGGCAGCCATTGCTAGCAAGCCTGCAAAACCACAGCCTAAGATGAAAACAAAGAGTGGGCCGATAGTTGG GGGTGCACTGCCTCCTCCCCCTATCAAGCATAACATGGACATTGGTACTTGGGATAACAAGGGCCCTGCT CCAAAGGCCTCCGCCCCCCAGCAGACACCATCCCCCCAGGCTGCCCCACAGCCCCAGCAGGTAGCTCAAC CTCTCCCTGTTCAGCCCCCACCTTTGGTCCAGCCACAGTATCAGAGCCCTCAGCAGCCACTTCAACCCCG CTGGGTGGCTCCTCGAAACAGAAATGCAGCATTTGGGCAGAGTGGAGGGGCCAACAGTGACAGTAACTCT GTTGGAAATGCCCAACCTACTTCTGCCCCGAGTGTAGAATCCCACCCTGTCCTGGAGAAACTGAAAGCTG CCCACAGCTATAACCCTAAAGAGTTCGACTGGAATCTTAAGAGTGGGCGGGTGTTCATCATCAAGAGCTA TTCTGAGGACGACATCCACCGCTCCATCAAGTACTCCATCTGGTGTAGTACTGAACACGGCAACAAGCGC CTGGACGGCGCCTTCCGCTCCATGAGCAGCAAGGGGCCTGTTTATCTCCTCTTCAGTGTCAATGGGAGTG GACATTTCTGTGGGGTGGCAGAGATGAAGTCCCCTGTGGACTACGGCACCAGCGCTGGGGTCTGGTCTCA GGACAAGTGGAAGGGAAAGTTTGATGTGAAGTGGATTTTTGTGAAGGATGTGCCCAACAACCAGCTGCGG CACATCAGACTGGAGAATAACGACAACAAACCTGTCACAAACTCCCGTGATACACAGGAGGTGCCCTTAG AAAAAGCAAAACAAGTGCTGAAGATTATCGCTTCCTATAAGCACACAACCTCTATCTTTGACGACTTTTC TCATTATGAGAAGCGCCAGGAGGAAGAGGAGGTGGTGCGTAAGGAAAGACAGAATCGAAACAAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208856 protein sequence
Red=Cloning site Green=Tags(s) MSATSVDPQRTKGQDNKVQNGSLHQKDAVHDNDFEPYLSGQSNPSNSYPSMSDPYLSSYYPPSIGFPYSL SEAPWSTAGDPPIPYLTTYGQLSNGDHHFMHDAVFGQPGGLGNNIYQHRFNFFPENPAFSAWGTSGSQGQ QTQSSAYGSSYTYPPSSLGGTVVDGQTGFHSDSLNKAPGMNSLEQGMVGLKIGDVTTSAVKTVGSVVNSV ALTGVLSGNGGTNVNMPVSKPTSWAAIASKPAKPQPKMKTKSGPIVGGALPPPPIKHNMDIGTWDNKGPA PKASAPQQTPSPQAAPQPQQVAQPLPVQPPPLVQPQYQSPQQPLQPRWVAPRNRNAAFGQSGGANSDSNS VGNAQPTSAPSVESHPVLEKLKAAHSYNPKEFDWNLKSGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKR LDGAFRSMSSKGPVYLLFSVNGSGHFCGVAEMKSPVDYGTSAGVWSQDKWKGKFDVKWIFVKDVPNNQLR HIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEEVVRKERQNRNKQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173761 |
| ORF Size | 1680 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173761.3, NP_776122.1 |
| RefSeq Size | 3199 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 228994 |
| UniProt ID | P59326 |
| MW | 60.9 kDa |
| Gene Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:30401835). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:30401835). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (By similarity). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (PubMed:30401835). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (PubMed:30843071). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (PubMed:30728504). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (PubMed:30728504). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (PubMed:30728504).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202640 | Ythdf1 (untagged) - Mouse YTH domain family 1 (Ythdf1), (10ug) |
CNY 2,000.00 |
|
| MG208856 | Ythdf1 (tGFP-tagged) - Mouse YTH domain family 1 (Ythdf1) |
CNY 7,856.00 |
|
| MR208856L3 | Lenti ORF clone of Ythdf1 (Myc-DDK-tagged) - Mouse YTH domain family 1 (Ythdf1) |
CNY 7,510.00 |
|
| MR208856L4 | Lenti ORF clone of Ythdf1 (mGFP-tagged) - Mouse YTH domain family 1 (Ythdf1) |
CNY 7,510.00 |
