Fbxl4 (BC033265) Mouse Tagged ORF Clone

CAT#: MR208744

  • TrueORF®

Fbxl4 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 4 (cDNA clone MGC:25568 IMAGE:3983942)

ORF Plasmid: DDK tGFP



  "BC033265" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,104.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 4732456I21, FBL4, FBL5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR208744 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGCCATAGGACTATAGTGTCAAACAGCCGGACTTCCCCTCTCAATGCAGAGGTGGTCCAGTATG
CTAAAGAAGTGGTGGATTTCAGTTCCCATTACGGCAGTGAGAACAGTATGTCCTATACCATGTGGAATTT
GGCAGGTGTACCAAATGTCTTCCCAAGTTCTGGTGACTTCACGCAGACGGCTGTGTTTCGAACTTATGGG
ACGTGGTGGGATCAGTGTCCTAGTGCTTCCTTGCCATTCAGAAGGACACCGTCCAGTTTCCAGAGCCAGG
ACTATGTGGAACTGACTTTTGAGCAGCAAGTGTACCCCACGGCTGTCCATGTGCTGGAGACTTACCATCC
TGGAGCAGTCATCAGAATTCTGGCCTGCTCAGCAAATCCCTACTCCCCAAATCCTCCAGCTGAAGTAAGA
TGGGAGATTCTTTGGTCAGAGAGACCTATGAAGGTGAATGCTTCCCAGGCTCGTCAGTTTAAACCTTGTA
TTAAGCAAATAAATTTCCCTACAAACCTCATACGACTGGAAGTCAATAGTTCTCTTCTGGATTATTACAC
CGAACTAGATGCAGTTGTGCTGCATGGTACGAAGGACAAGCCACTGCTCTCTCTCAAGACTGCTCTTGTA
GACATGAATGATCTAGAAGATGACGACTATGAAGAGAAGGATGGCTGTGAGATGGATGCTCTTAACAAAA
AGTTTAGCAGTGCTGCCCTTGGGGATGGGCCACATAATGGGTATTTTGATAAACTACCATATGAGCTCAT
TCAACTGATTCTGAATCATCTTTCACTACCAGACCTGTGTAGATTAGCCCAGACTTGCAGGCTCCTCCAC
CAGCATTGCTGTGATCCTCTGCAATATATCCACCTCAACCTGCAGCCATACTGGGCCCGGCTGGACGACA
CGTCACTGGAGTTCCTACAGGCTCGCTGTGTGCTTGTCCAGTGGCTTAATCTGTCTTGGACTGGCAACAG
GGGGTTCATCTCTGTATCAGGATTTAGCAGGTTTCTAAAGGTGTGTGGGTCTGAGTTAGTACGCCTTGAG
TTATCCTGCAGCCACTTTCTTAATGACACTTGCTTGGAGGTTATTTCTGAAATGTGCCCAAATCTACAAG
ACTTAAATCTCTCATCCTGCGATAAACTCCCACCTCAGGCTTTTGGCCATATTGCCAAGTTATGCAGCCT
CAAGAGGCTTGTTCTCTATCGGACAAAAGTAGAGCAAACAGCACTACTGAGCATTTTGAACTTCTGTGCA
GAGCTTCAGCACCTCAGCCTGGGCAGCTGTGTGATGATTGAAGATTATGATGTGATAGCCAGCATGATAG
GAGCCAAGTGTAAAAACCTGCGGACTTTGGATCTGTGGAGATGCAAGAATATCACCGAGAATGGGATAGC
AGAGTTGGCTTCTGGCTGTGTTCTTCTTGAGGAACTTGACCTTGGCTGGTGCCCCACCCTTCAGAGCAGC
ACTGGGTGCTTTGCCAGACTGGCACGCCAGCTCCCAAACTTGCAGAAACTCTTTCTTACAGCTAACAGGT
CTGTGTGTGACACAGACATAGAAGAACTGGCATCTAATTGTACCAGATTACAGCAACTGGACATATTAGG
CCTGAAACAGGTTTTCAAAACTGTTAGGATAGTTGCTCCGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR208744 protein sequence
Red=Cloning site Green=Tags(s)

MNSHRTIVSNSRTSPLNAEVVQYAKEVVDFSSHYGSENSMSYTMWNLAGVPNVFPSSGDFTQTAVFRTYG
TWWDQCPSASLPFRRTPSSFQSQDYVELTFEQQVYPTAVHVLETYHPGAVIRILACSANPYSPNPPAEVR
WEILWSERPMKVNASQARQFKPCIKQINFPTNLIRLEVNSSLLDYYTELDAVVLHGTKDKPLLSLKTALV
DMNDLEDDDYEEKDGCEMDALNKKFSSAALGDGPHNGYFDKLPYELIQLILNHLSLPDLCRLAQTCRLLH
QHCCDPLQYIHLNLQPYWARLDDTSLEFLQARCVLVQWLNLSWTGNRGFISVSGFSRFLKVCGSELVRLE
LSCSHFLNDTCLEVISEMCPNLQDLNLSSCDKLPPQAFGHIAKLCSLKRLVLYRTKVEQTALLSILNFCA
ELQHLSLGSCVMIEDYDVIASMIGAKCKNLRTLDLWRCKNITENGIAELASGCVLLEELDLGWCPTLQSS
TGCFARLARQLPNLQKLFLTANRSVCDTDIEELASNCTRLQQLDILGLKQVFKTVRIVAPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC033265
ORF Size 1653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC033265, AAH33265
RefSeq Size 2620 bp
RefSeq ORF 1655 bp
Locus ID 269514
MW 62.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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