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Slc1a3 (NM_148938) Mouse Tagged ORF Clone
CAT＃: MR208665
- TrueORF®
Slc1a3 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3)
ORF Plasmid: tGFP
"NM_148938" in other vectors (3)
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CNY 6,064.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI504299; B430115D02Rik; Eaat1; GLAST; GLAST-1; GLU-T; GluT-1; Gmt1; MGluT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208665 representing NM_148938
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAAAGCAACGGAGAAGAGCCTAGGATGGGGGGCAGGATGGAGAGATTGCAGCAAGGGGTCCGCA AGCGGACACTTCTGGCCAAGAAGAAAGTTCAGAGCCTCACCAAGGAAGATGTTAAGAGTTACCTGTTTCG GAATGCCTTCGTTCTGCTCACGGTCACTGCTGTCATTGTGGGTACAATCCTTGGATTTGCCCTCCGACCG TATAAAATGAGCTACCGGGAGGTGAAGTACTTTTCGTTCCCTGGGGAGCTTCTCATGAGGATGCTGCAGA TGCTGGTCTTGCCCCTGATCATCTCCAGTCTCGTCACAGGAATGGCGGCCCTAGATAGTAAGGCATCCGG GAAGATGGGGATGCGCGCTGTAGTCTATTACATGACTACTACCATCATTGCTGTGGTGATTGGCATAATC ATTGTCATCATCATCCACCCCGGAAAGGGCACAAAGGAAAACATGTACAGAGAAGGTAAAATCGTGCAGG TCACTGCAGCAGATGCCTTCCTGGATTTGATCAGGAACATGTTCCCTCCCAATCTGGTAGAAGCCTGCTT TAAACAGTTTAAAACCAGCTACGAGAAAAGAAGCTTTAAAGTGCCTATCCAGTCCAACGAAACACTTCTG GGCGCCGTGATCAACAACGTGTCAGAGGCCATGGAGACTCTGACCCGGATCCGGGAGGAGATGGTGCCCG TGCCTGGATCTGTGAATGGGGTCAATGCCCTGGGCCTAGTTGTCTTCTCCATGTGCTTCGGTTTCGTGAT CGGAAACATGAAGGAGCAGGGGCAAGCGCTGAGAGAGTTCTTTGATTCTCTTAACGAAGCCATCATGCGA TTGGTCGCGGTGATAATGTGGTATGCGCCTCTGGGCATCCTCTTCTTGATCGCAGGGAAGATTGTTGAGA TGGAAGACATGGGTGTGATTGGGGGACAGCTTGCCATGTACACCGTGACAGTCATTGTCGGCCTCCTCAT TCACGCCGTCATCGTCCTGCCTCTCCTCTACTTCCTGGTAACCCGGAAGAACCCCTGGGTTTTCATTGGA GGGTTGCTGCAAGCGCTCATCACAGCCCTTGGGACCTCCTCAAGTTCTGCCACCCTACCCATCACTTTCA AGTGCCTGGAAGAGAACAATGGTGTGGACAAACGCATCACCAGATTTGTGCTCCCCGTGGGGGCCACCAT TAACATGGATGGGACCGCCCTCTACGAGGCTTTGGCTGCCATTTTCATCGCTCAAGTGAACAACTTTGAC CTGAACTTTGGACAGATTATAACAATAAGCATCACAGCCACGGCCGCAAGCATCGGGGCAGCCGGGATTC CTCAGGCCGGTCTGGTCACCATGGTCATCGTGCTGACATCTGTGGGCCTGCCCACAGATGACATCACACT CATCATTGCAGTGGACTGGTTTCTGGACCGCCTCCGAACCACCACCAACGTACTGGGTGACTCCCTCGGA GCAGGGATTGTCGAGCACTTGTCCCGACATGAACTGAAGAACCGAGATGTTGAAATGGGGAACTCGGTGA TTGAGGAGAACGAAATGAAGAAGCCGTATCAGCTGATTGCCCAGGACAATGAACCGGAGAAACCCGTGGC AGACAGCGAAACCAAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208665 representing NM_148938
Red=Cloning site Green=Tags(s) MTKSNGEEPRMGGRMERLQQGVRKRTLLAKKKVQSLTKEDVKSYLFRNAFVLLTVTAVIVGTILGFALRP YKMSYREVKYFSFPGELLMRMLQMLVLPLIISSLVTGMAALDSKASGKMGMRAVVYYMTTTIIAVVIGII IVIIIHPGKGTKENMYREGKIVQVTAADAFLDLIRNMFPPNLVEACFKQFKTSYEKRSFKVPIQSNETLL GAVINNVSEAMETLTRIREEMVPVPGSVNGVNALGLVVFSMCFGFVIGNMKEQGQALREFFDSLNEAIMR LVAVIMWYAPLGILFLIAGKIVEMEDMGVIGGQLAMYTVTVIVGLLIHAVIVLPLLYFLVTRKNPWVFIG GLLQALITALGTSSSSATLPITFKCLEENNGVDKRITRFVLPVGATINMDGTALYEALAAIFIAQVNNFD LNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTDDITLIIAVDWFLDRLRTTTNVLGDSLG AGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNEPEKPVADSETKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_148938 |
| ORF Size | 1629 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_148938.3, NP_683740.1 |
| RefSeq Size | 4214 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 20512 |
| UniProt ID | P56564 |
| MW | 60.1 kDa |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7903437, PubMed:28032905). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (PubMed:15363892, PubMed:15390100,PubMed:16880397).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
GltI and Glast negatively regulate calcium-dependent proliferation of hippocampal neural progenitor cells and are persistently upregulated after injury
,null,
The European journal of neuroscience
,PubMed ID 22092549
[Slc1a3]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208665 | Slc1a3 (tGFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3) |
CNY 5,990.00 |
|
| MR208665L3 | Lenti ORF clone of Slc1a3 (Myc-DDK-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3) |
CNY 8,464.00 |
|
| MR208665L4 | Lenti ORF clone of Slc1a3 (mGFP-tagged) - Mouse solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3) |
CNY 8,464.00 |
