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Cnot2 (BC063105) Mouse Tagged ORF Clone
CAT＃: MR208509
- TrueORF®
Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026)
ORF Plasmid: tGFP
"BC063105" in other vectors (4)
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CNY 3,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2600016M12Rik; 2810470K03Rik; AA537049; AA959607; AW557563; C79650 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208509 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAAGAAGTTGCACAGGTGACAAACAGCATGTTTGGTGCTTCAAGAAAGAAGTTTGTAGAGGGGG TGGACAGCGACTACCATGATGAGAACATGTACTACAGCCAGTCTTCTATGTTCCCACATCGGTCAGAGAA AGATATGCTGGCATCGCCATCTACGTCAGGTCAGCTGTCTCAATTTGGGGCAAGTTTATACGGGCAACAA AGTGCACTAGGCCTTCCAATGAGGGGGATGAGCAACAATACCCCTCAGTTAAATCGCAGCTTATCACAAG GCACTCAGTTACCGAGCCACGTCACGCCAACAACAGGGGTACCAACAATGTCACTTCACACGCCTCCATC TCCAAGCAGGGGTATTTTGCCTATGAATCCTAGGAATATGATGAACCACTCCCAGGTTGGTCAGGGCATT GGAATTCCTAGCAGGACAAATAGCATGAGCAGTTCAGGGTTAGGTAGCCCCAACAGAAGCTCGCCAAGCA TAATATGTATGCCAAAGCAACAGCCTTCTCGACAGCCTTTTACTGTGAACAGTATGTCTGGATTTGGAAT GAACAGGAATCAGGCATTTGGAATGAATAACTCCTTATCAAGTAACATTTTTAATGGAACAGATGGCAGT GAAAATGTGACAGGATTGGACCTTTCAGATTTCCCAGCGTTAGCTGACCGGAATAGAAGGGAAGGGAGCG GCAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCCTTACGTTGGAATGGTAACAAAACCTGC AAATGAGCAATCCCAAGACTTCTCAATACACAACGAAGATTTTCCAGCATTACCTGGTTCCAGCTATAAA GATCCAACGTCAAGTAATGACGACAGCAAATCTAATTTGAGTACATCGGGGAAGACGACTTCAAGTACAG ATGGACCCAAATTCCCTGGAGATAAAAGTTCAACAACACAAAACAATAATCAACAGAAAAAGGGGATCCA GGTGTTACCTGATGGTCGAGTTACTAACATTCCTCAAGGGATGGTGACGGACCAATTTGGAATGATTGGC CTGTTAACATTTATCAGGGCAGCAGAGACAGACCCAGGAATGGTACATCTTGCTTTAGGAAGTGACTTAA CAACATTAGGCCTCAATCTGAACTCACCTGAAAATCTCTATCCCAAATTTGCATCACCCTGGGCTTCTTC ACCTTGTCGGCCTCAAGACATAGACTTCCATGTTCCATCAGAATATTTAACAAACATTCACATTAGGGAT AAGCTGGCTGCAATCAAACTTGGCCGATATGGAGAAGACCTCCTCTTCTATCTGTATTACATGAACGGCG GGGATGTATTACAACTCTTAGCTGCGGTAGAACTTTTTAACCGTGATTGGAGATACCACAAGGAGGAGCG GGTATGGATTACCAGGGCACCAGGCATGGAGCCAACAATGAAAACCAACACGTATGAGCGGGGGACCTAC TATTTCTTTGACTGTCTCAACTGGAGGAAAGTAGCTAAGGAGTTCCATCTGGAATATGACAAATTAGAAG AGCGGCCTCACCTGCCATCCACCTTCAACTACAACCCTGCTCAGCAAGCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208509 protein sequence
Red=Cloning site Green=Tags(s) MLKEVAQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQ SALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGI GIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGS ENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYK DPTSSNDDSKSNLSTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIG LLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRD KLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTY YFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC063105 |
| ORF Size | 1593 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC063105, AAH63105 |
| RefSeq Size | 3014 bp |
| RefSeq ORF | 1595 bp |
| Locus ID | 72068 |
| MW | 58.6 kDa |
| Gene Summary | Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217882 | Cnot2 (untagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026), (10ug) |
CNY 6,940.00 |
|
| MG208509 | Cnot2 (tGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026) |
CNY 3,330.00 |
|
| MR208509L3 | Lenti ORF clone of Cnot2 (Myc-DDK-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026) |
CNY 4,940.00 |
|
| MR208509L4 | Lenti ORF clone of Cnot2 (mGFP-tagged) - Mouse CCR4-NOT transcription complex, subunit 2 (cDNA clone MGC:70296 IMAGE:5003026) |
CNY 4,940.00 |
