Traf6 (NM_009424) Mouse Tagged ORF Clone
CAT#: MR208489
- TrueORF®
Traf6 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 6 (Traf6)
ORF Plasmid: tGFP
"NM_009424" in other vectors (4)
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CNY 5,928.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310003F17Rik; AI851288; C630032O20Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208489 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCTCTTAAACTGTGAGAACAGCTGCGGGTCCAGCCAGTCGTCCAGTGACTGCTGCGCTGCCATGG CCGCCTCCTGCAGCGCTGCAGTGAAAGATGACAGCGTGAGTGGCTCTGCCAGCACCGGGAACCTCTCCAG CTCCTTCATGGAGGAGATCCAGGGCTACGATGTGGAGTTTGACCCACCTCTGGAGAGCAAGTATGAGTGT CCCATCTGCTTGATGGCTTTACGGGAAGCAGTGCAAACACCATGTGGCCACAGGTTCTGCAAAGCCTGCA TCATCAAATCCATAAGGGATGCAGGGCACAAGTGCCCAGTTGACAATGAAATACTGCTGGAAAATCAACT GTTTCCCGACAATTTTGCAAAGCGAGAGATTCTTTCCCTGACGGTAAAGTGCCCAAATAAAGGCTGTTTG CAAAAGATGGAACTGAGACATCTCGAGGATCATCAAGTACATTGTGAATTTGCTCTAGTGAATTGTCCCC AGTGCCAACGTCCTTTCCAGAAGTGCCAGGTTAATACACACATTATTGAGGATTGTCCCAGGAGGCAGGT TTCTTGTGTAAACTGTGCTGTGTCCATGGCATATGAAGAGAAAGAGATCCATGATCAAAGCTGTCCTCTG GCAAATATCATCTGTGAATACTGTGGTACAATCCTCATCAGAGAACAGATGCCTAATCATTATGATCTGG ACTGCCCAACAGCTCCAATCCCTTGCACATTCAGTGTTTTTGGCTGTCATGAAAAGATGCAGAGGAATCA CTTGGCACGACACTTGCAAGAGAATACCCAGTTGCACATGAGACTGTTGGCCCAGGCTGTTCATAATGTT AACCTTGCTTTGCGTCCGTGCGATGCCGCCTCTCCATCCCGGGGATGTCGTCCAGAGGACCCAAATTATG AGGAAACTATCAAACAGTTGGAGAGTCGCCTAGTAAGACAGGACCATCAGATCCGGGAGCTGACTGCCAA AATGGAAACTCAGAGTATGTACGTGGGCGAGCTCAAACGGACCATTCGGACCCTGGAGGACAAGGTTGCC GAAATGGAAGCACAGCAGTGTAACGGGATCTACATTTGGAAGATTGGCAACTTTGGGATGCACTTGAAAT CCCAAGAAGAGGAAAGACCTGTTGTCATCCATAGCCCTGGATTCTACACAGGCAGACCTGGGTACAAGCT GTGCATGCGCCTGCATCTTCAGTTACCGACAGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCAC ACAATGCAAGGAGAATATGACAGCCACCTCCCCTGGCCCTTCCAGGGTACAATACGCCTTACAATTCTCG ACCAGTCTGAAGCACTTATAAGGCAAAACCACGAAGAGGTCATGGACGCCAAACCAGAACTGCTTGCCTT TCAGCGACCCACAATCCCACGGAACCCCAAAGGTTTTGGCTATGTAACATTTATGCACCTGGAAGCCTTA AGACAGGGAACCTTCATTAAGGATGATACATTACTAGTGCGCTGTGAAGTCTCTACCCGCTTTGACATGG GTGGCCTTCGGAAGGAGGGTTTCCAGCCACGAAGTACTGATGCGGGGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208489 protein sequence
Red=Cloning site Green=Tags(s) MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYEC PICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCL QKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPL ANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNV NLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVA EMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVH TMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEAL RQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009424 |
ORF Size | 1593 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009424.3, NP_033450.2 |
RefSeq Size | 5536 bp |
RefSeq ORF | 1593 bp |
Locus ID | 22034 |
UniProt ID | P70196 |
MW | 60.1 kDa |
Gene Summary | This gene encodes a member of the TNF receptor associated factor (TRAF) family of adaptor proteins that mediate signaling events from members of the TNF receptor and Toll/IL-1 receptor families to activate transcription factors such as NF-kappa-B and AP-1. The product of this gene is essential for perinatal and postnatal survival. Mice deficient in this protein exhibit osteopetrosis and defective in development of epidermal appendixes, normal B cell differentiation, lymph node organogenesis, interleukin-1 signaling, lipopolysaccharide signaling and neural tube closure. This protein possesses ubiquitin ligase activity. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
The LPS-inducible lncRNA Mirt2 is a negative regulator of inflammation
,Du, M;Yuan, L;Tan, X;Huang, D;Wang, X;Zheng, Z;Mao, X;Li, X;Yang, L;Huang, K;Zhang, F;Wang, Y;Luo, X;Huang, D;Huang, K;,
Nat Commun
,PubMed ID 29230038
[Traf6]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217548 | Traf6 (untagged) - Mouse TNF receptor-associated factor 6 (Traf6), (10ug) |
CNY 6,840.00 |
|
MG208489 | Traf6 (tGFP-tagged) - Mouse Tnf receptor-associated factor 6 (Traf6) |
CNY 7,528.00 |
|
MR208489L3 | Lenti ORF clone of Traf6 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 6 (Traf6) |
CNY 8,328.00 |
|
MR208489L4 | Lenti ORF clone of Traf6 (mGFP-tagged) - Mouse TNF receptor-associated factor 6 (Traf6) |
CNY 8,328.00 |