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2010005J08Rik (BC066994) Mouse Tagged ORF Clone
CAT＃: MR208284
- TrueORF®
2010005J08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010005J08 gene (cDNA clone MGC:91347 IMAGE:5696000)
ORF Plasmid: tGFP
"BC066994" in other vectors (4)
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CNY 3,848.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010005J08Rik; 9130001I21Rik; AW060359; mKIAA1507; Urg4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208284 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGAGATGACTGCGGGTTCCATTATGGAGATGGTACAAACGAGGCTCAGGACAATGACTTCCCCA CAGTGGAGAGAAGCAGGCTTCAGGAGATGCTGTCCCTGTTGGGACTGGAGACATACCAGGCACAGAAGCT CACCCTCCAGGACTCCCTGCAGATCAGTTTTGACAGCATGAAGAACTGGGCTCCTCAGGTTCCCAAGGAC CTTCCCTGGCATTTCCTCAGGAAGCTGCAGGCACTCAACGCCGAAGCCAGGAACACCACCATGGTGCTGG ACGTGCCTCCAGAGGCCTGGCCTGCAGAGAAGGAGAGCCAGGCAGAGGAGGAGATGATGTACTGGGACCC AGCGGAGGACGTCGCTGCTGACATCTACTCTTTCTCAGAGCTGCCCATGCCTGATACGCCTGTGAACCCC CTGGATCTTCTCTGCGCCCTCCTGCTGTCTTCAGACACCTTTCTGCAGCAGGAAGTCCTGCTGAAGATGT CCCTCTGTCAGTTTGCACTCCCACTTGTGTTGCCCGATTCTGAAAACCACTACCACACCTTTCTGCTCTG GGCCCTGCGGGGTGTTGTGCGGACGTGGTGGTTCCAGCCCCTCCGGAGCCTGGGGAGTTTCCGAGAAGAC AGTGTGGTCCTGTCCAGGGTGCCCACGTTTGCCTTCGTGCGCATGGATGTCAGCAGCAACTCCAAGTCCC AGCTGCTCAATGCCGTCCTCAGCCCGGCCCGCCGCCAGTGGGACTGCTTCTGGCATCGGGACCTCAATTT AGGCACTAATCCTCGCGAGATCTCGGATGGGTTAGTAGAGATCTCCTGGTTTTTCCCCAGTGGTAAGGAG GACCTGGATGTTTTCCCGGAGCCCATGGCCTTTCTGAACTTGAGAGGTGACATTGGGTCTCACTGGCTGC AGTTCAAGCTCTTGACAGAAGTCTCCTCTGCTGTGTTTATTTTGACTGACAACATCAGCAAGAAGGAATA CAAACTGCTGTACTCCATGAAGGAGTCAACCACAAAGTACTACTTCATCCTGAGTCCCTACCGAGGGAAA CGGAACACGAACCTGCGCTTCCTCAACAGGCTGATCCCAGTGTTGAAGATAGACCACTCTCACGTCCTGG TGAAGGTCAGCAGCACGGACAGCGAGGGCTTCGTGAGGCGCATCCGAGCCATCATGTGCAGTGTGGCTCG CTCTCCCTGCAGGAGGGTGTCTGTGGAGGACATGGCACACGCGGCACGTAAGCTCGGCCTCAGAGTTGAT GAGGACTTTGAGGAGTGTCAGAAGGCAAAGGACCGGATGGAGAAAATCCTCAGGAAGATCAAGGATGTGG ACGCCTACAAGAGAGACGAGCTGCGGCTGCAGGGCGAGCCTGCGAGGAGGGCCGCCCAAGCTGAAAGAGA GTTCTGCCAGCTCCAGTGGGTCCCGGAGCCCCCAGAGAAGCACCGGGCCGAGCTGAGACGGCGCGTCCTT GAGCTCCGGGTGCAGCAGAATGGCCAGGAGCCCACCTCAGGGGTCCAGGAGTTCATCCTGGGAATAAGCA GCCCCTCCCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208284 protein sequence
Red=Cloning site Green=Tags(s) MEGDDCGFHYGDGTNEAQDNDFPTVERSRLQEMLSLLGLETYQAQKLTLQDSLQISFDSMKNWAPQVPKD LPWHFLRKLQALNAEARNTTMVLDVPPEAWPAEKESQAEEEMMYWDPAEDVAADIYSFSELPMPDTPVNP LDLLCALLLSSDTFLQQEVLLKMSLCQFALPLVLPDSENHYHTFLLWALRGVVRTWWFQPLRSLGSFRED SVVLSRVPTFAFVRMDVSSNSKSQLLNAVLSPARRQWDCFWHRDLNLGTNPREISDGLVEISWFFPSGKE DLDVFPEPMAFLNLRGDIGSHWLQFKLLTEVSSAVFILTDNISKKEYKLLYSMKESTTKYYFILSPYRGK RNTNLRFLNRLIPVLKIDHSHVLVKVSSTDSEGFVRRIRAIMCSVARSPCRRVSVEDMAHAARKLGLRVD EDFEECQKAKDRMEKILRKIKDVDAYKRDELRLQGEPARRAAQAEREFCQLQWVPEPPEKHRAELRRRVL ELRVQQNGQEPTSGVQEFILGISSPSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC066994 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066994, AAH66994 |
| RefSeq Size | 3342 bp |
| RefSeq ORF | 1553 bp |
| Locus ID | 72046 |
| MW | 59.7 kDa |
| Gene Summary | May be involved in cell cycle progression through the regulation of cyclin D1 expression.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218438 | 2010005J08Rik (untagged) - Mouse RIKEN cDNA 2010005J08 gene (cDNA clone MGC:91347 IMAGE:5696000), (10ug) |
CNY 6,940.00 |
|
| MG208284 | 2010005J08Rik (tGFP-tagged) - Mouse RIKEN cDNA 2010005J08 gene (cDNA clone MGC:91347 IMAGE:5696000) |
CNY 3,230.00 |
|
| MR208284L3 | Lenti ORF clone of 2010005J08Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010005J08 gene (cDNA clone MGC:91347 IMAGE:5696000) |
CNY 4,850.00 |
|
| MR208284L4 | Lenti ORF clone of 2010005J08Rik (mGFP-tagged) - Mouse RIKEN cDNA 2010005J08 gene (cDNA clone MGC:91347 IMAGE:5696000) |
CNY 4,850.00 |
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