United States
Germany
Japan
United Kingdom
China
Apex2 (NM_029943) Mouse Tagged ORF Clone
CAT＃: MR208278
- TrueORF®
Apex2 (Myc-DDK-tagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2)
ORF Plasmid: tGFP
"NM_029943" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,848.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ape2; C430040P13Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208278 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCGTGGTAAGCTGGAACATCAATGGGATCCGGAGTCCCCTGCAAGGCCTTGCATGCCAGGAAC CCAGCAGCTGTCCCACGGCCTTGCGACGCGTTTTGGACGAGCTGGATGCTGATATTGTCTGTCTCCAGGA GACCAAAGTGACCAGAGATGTACTGACAGAGCCCCTGGCTATTGTTGAGGGTTATAACTCCTATTTCAGC TTCAGCCGCAGCCGTAGTGGCTATTCTGGTGTGGCTACCTTCTGTAAGGACAGTGCTACCCCAGTAGCTG CTGAAGAAGGCCTGAGTGGTGTGTTTGCCACCCTGAATGGAGATATTGGTTGCTATGGAAACATGGATGA GTTCACGCAAGAGGAACTCCGGGTTCTGGATAGTGAGGGCCGGGCCCTCCTTACACAGCACAAGATCCGC ACATTGGAAGGGAAGGAGAAGACTTTGACCCTGATCAATGTGTACTGCCCTCACGCGGATCCTGGGAAGC CTGAGCGGCTGACCTTCAAGATGCGCTTCTATCGCCTGCTCCAGATGCGAGCAGAAGCGCTCCTGGCAGC TGGCAGTCACGTGATAATCCTGGGGGACCTGAATACAGCCCACCGACCCATTGACCACTGCGATGCAAGT AGTCTGGAATGCTTTGAAGAGGACCCAGGGCGTAAGTGGATGGATGGCTTGCTCAGTAACCCAGGGGATG AGGCTGGACCCCACATAGGGCTCTTCATGGATAGCTACCGCTACTTGCATCCAAAACAGCAGAGGGCTTT CACCTGCTGGTCAGTGGTCAGTGGTGCACGCCATCTCAACTATGGCTCTAGACTTGACTATGTACTGGGA GATAGGGCCTTGGTCATAGATACCTTCCAGGCCTCCTTCTTACTCCCTGAAGTGATGGGCTCTGACCACT GCCCTGTGGGAGCTGTCTTAAATGTATCCTGTGTGCCAGCAAAACAGTGCCCAGCTCTGTGTACCCGCTT CCTCCCCGAGTTTGCAGGTACCCAGCTCAAGATTCTTCGCTTCCTAGTTCCTCTTGAGCAAGAACCTGTG CGGGAACAGCAAGTCCTGCAGCCCAGCCATCAAATCCAGGCACAGAGACAGCCAAGAAAAGCCTGCATGC ATTCAACCAGGCTTCGGAAAAGTCAAGGTGGCCCCAAAAGAAAACAGAAAAACCTGATGAGTTACTTCCA GCCTTCCTCCAGCCTTTCCCAAACTTCTGGTGTGGAGCTGCCTACCCTGCCTCTGGTGGGCCCTCTTACA ACCCCAAAGACTGCAGAAGAGGTGGCAACAGCCACAGTGCTAGAAGAGAAGAACAAGGTTCCAGAGTCCA AAGATGAGAAGGGAGAAAGGACTGCCTTCTGGAAGTCTATGCTGAGTGGGCCCTCACCCATGCCCCTCTG TGGAGGCCACAGGGAGCCATGTGTGATGCGTACTGTGAAAAAAACAGGACCCAACTTTGGTCGCCAGTTC TACATGTGTGCTAGACCCCGAGGTCCTCCCAGTGACCCCTCTTCCCGCTGTAACTTCTTCCTCTGGAGCA GGCCCAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR208278 protein sequence
Red=Cloning site Green=Tags(s) MLRVVSWNINGIRSPLQGLACQEPSSCPTALRRVLDELDADIVCLQETKVTRDVLTEPLAIVEGYNSYFS FSRSRSGYSGVATFCKDSATPVAAEEGLSGVFATLNGDIGCYGNMDEFTQEELRVLDSEGRALLTQHKIR TLEGKEKTLTLINVYCPHADPGKPERLTFKMRFYRLLQMRAEALLAAGSHVIILGDLNTAHRPIDHCDAS SLECFEEDPGRKWMDGLLSNPGDEAGPHIGLFMDSYRYLHPKQQRAFTCWSVVSGARHLNYGSRLDYVLG DRALVIDTFQASFLLPEVMGSDHCPVGAVLNVSCVPAKQCPALCTRFLPEFAGTQLKILRFLVPLEQEPV REQQVLQPSHQIQAQRQPRKACMHSTRLRKSQGGPKRKQKNLMSYFQPSSSLSQTSGVELPTLPLVGPLT TPKTAEEVATATVLEEKNKVPESKDEKGERTAFWKSMLSGPSPMPLCGGHREPCVMRTVKKTGPNFGRQF YMCARPRGPPSDPSSRCNFFLWSRPS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029943 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029943.1, NM_029943.2, NP_084219.1 |
| RefSeq Size | 1903 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 77622 |
| UniProt ID | Q68G58 |
| MW | 57.3 kDa |
| Gene Summary | Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204878 | Apex2 (untagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2), (10ug) |
CNY 2,000.00 |
|
| MG208278 | Apex2 (tGFP-tagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2) |
CNY 5,040.00 |
|
| MR208278L3 | Lenti ORF clone of Apex2 (Myc-DDK-tagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2) |
CNY 6,460.00 |
|
| MR208278L4 | Lenti ORF clone of Apex2 (mGFP-tagged) - Mouse apurinic/apyrimidinic endonuclease 2 (Apex2) |
CNY 6,460.00 |
