Slc2a4 (NM_009204) Mouse Tagged ORF Clone
CAT#: MR208202
- TrueORF®
Slc2a4 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4)
ORF Plasmid: tGFP
"NM_009204" in other vectors (3)
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CNY 3,792.00
CNY 4,470.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Glut-4; Glut4; GT2; twgy |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR208202 representing NM_009204
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTCGGGTTTCCAGCAGATCGGCTCTGACGATGGGGAACCCCCTCGGCAGCGAGTGACTGGAACAC TGGTCCTAGCTGTATTCTCAGCTGTGCTTGGCTCCCTTCAGTTTGGCTATAACATTGGGGTTATCAATGC CCCACAGAAGGTGATTGAACAGAGCTACAATGCAACGTGGCTGGGTAGGCAAGGTCCTGGGGGACCGGAT TCCATCCCACAAGGCACCCTCACTACGCTCTGGGCTCTCTCCGTGGCCATCTTCTCTGTGGGTGGCATGA TCTCTTCCTTTCTCATTGGCATCATTTCTCAATGGTTGGGAAGGAAAAGGGCTATGCTGGCCAACAATGT CTTGGCCGTGTTGGGGGGCGCCCTCATGGGCCTAGCCAATGCCGCGGCCTCCTATGAGATACTCATTCTT GGACGGTTCCTCATTGGCGCCTACTCAGGGCTAACATCAGGGCTGGTGCCCATGTATGTGGGAGAAATCG CCCCCACTCATCTTCGGGGTGCCTTGGGAACACTCAACCAACTGGCCATCGTCATTGGCATTCTGGTTGC CCAGGTGCTGGGCTTGGAGTCTATGCTGGGCACAGCTACCCTGTGGCCACTGCTTCTGGCTCTCACAGTA CTCCCTGCTCTCCTGCAGCTGATTCTGCTGCCCTTCTGTCCTGAGAGCCCCAGATACCTCTACATCATCC GGAACCTGGAGGGGCCTGCCCGAAAGAGTCTAAAGCGCCTGACCGGCTGGGCTGATGTGTCTGACGCACT AGCTGAGCTGAAGGATGAGAAACGGAAGTTGGAGAGAGAGCGTCCAATGTCCTTGCTCCAGCTCCTGGGC AGCCGCACCCACCGGCAGCCTCTGATCATCGCAGTGGTGCTGCAGCTGAGCCAACAGCTCTCAGGCATCA ATGCTGTTTTCTACTATTCAACCAGCATCTTCGAGTCGGCTGGGGTGGGACAGCCAGCCTACGCCACCAT AGGAGCTGGTGTGGTCAATACGGTCTTCACGTTGGTCTCGGTGCTCTTAGTAGAACGAGCTGGACGACGG ACACTCCATCTGTTGGGCCTGGCCGGCATGTGTGGCTGTGCCATCTTGATGACCGTGGCTCTGCTGCTGC TGGAACGGGTTCCAGCCATGAGCTATGTCTCCATCGTGGCCATATTTGGCTTTGTGGCCTTCTTTGAGAT TGGCCCTGGCCCCATTCCCTGGTTCATTGTGGCAGAGCTCTTCAGCCAGGGCCCCCGCCCAGCCGCCATG GCTGTCGCTGGTTTCTCCAACTGGACCTGTAACTTCATTGTCGGCATGGGTTTCCAGTATGTTGCGGATG CTATGGGTCCTTACGTCTTCCTTCTATTTGCCGTCCTCCTGCTTGGCTTCTTCATCTTCACCTTCCTAAA AGTGCCTGAAACCAGAGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCGACGGACACCTTCCCTTTTA GAGCAGGAGGTGAAACCCAGTACAGAACTTGAATACTTAGGGCCAGATGAGAATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208202 representing NM_009204
Red=Cloning site Green=Tags(s) MPSGFQQIGSDDGEPPRQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNATWLGRQGPGGPD SIPQGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLANNVLAVLGGALMGLANAAASYEILIL GRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILVAQVLGLESMLGTATLWPLLLALTV LPALLQLILLPFCPESPRYLYIIRNLEGPARKSLKRLTGWADVSDALAELKDEKRKLERERPMSLLQLLG SRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFESAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRR TLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM AVAGFSNWTCNFIVGMGFQYVADAMGPYVFLLFAVLLLGFFIFTFLKVPETRGRTFDQISAAFRRTPSLL EQEVKPSTELEYLGPDEND TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_009204 |
ORF Size | 1527 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_009204.2, NP_033230.2 |
RefSeq Size | 2822 bp |
RefSeq ORF | 1530 bp |
Locus ID | 20528 |
UniProt ID | P14142 |
MW | 55.2 kDa |
Gene Summary | Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed:26240143, PubMed:26629404). Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed:26240143, PubMed:26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed:26240143, PubMed:26629404).[UniProtKB/Swiss-Prot Function] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
The myokine meteorin-like (metrnl) improves glucose tolerance in both skeletal muscle cells and mice by targeting AMPKα2
,Lee, JO;Byun, WS;Kang, MJ;Han, JA;Moon, J;Shin, MJ;Lee, HJ;Chung, JH;Lee, JS;Son, CG;Song, KH;Kim, TW;Lee, ES;Kim, HM;Chung, CH;Ngoei, KRW;Ling, NXY;Oakhill, JS;Galic, S;Segal, LM;Kemp, BE;Kim, KM;Lim, S;Kim, HS;,
FEBS J.
,PubMed ID 32196931
[SLC2A4]
|
Deletion of Sulfonylurea Receptor 2 in the Adult Myocardium Enhances Cardiac Glucose Uptake and Is Cardioprotective
,null,
JACC: Basic to Translational Science
,PubMed ID 31061927
[Slc2a4]
|
Meteorin-like (Metrnl) adipomyokine improves glucose tolerance in type 2 diabetes via AMPK pathway
,Kim, H;Lee, J;Lee, H;Lee, Y;Han, J;Kang, M;Moon, J;Shin, M;Lee, H;Chung, J;Lee, J;Son, C;Song, K;Kim, T;Lee, E;Kim, H;Chung, C;Ngoei, K;Ling, N;Oakhill, J;Galic, S;Segal, L;Kemp, B;Kim, K;Lim, S;,
bioRxiv
[SLC2A4]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG208202 | Slc2a4 (tGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4) |
CNY 5,392.00 |
|
MR208202L3 | Lenti ORF clone of Slc2a4 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4) |
CNY 6,370.00 |
|
MR208202L4 | Lenti ORF clone of Slc2a4 (mGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4) |
CNY 6,192.00 |