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Tle6 (BC048832) Mouse Tagged ORF Clone
CAT＃: MR208164
- TrueORF®
Tle6 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029)
ORF Plasmid: tGFP
"BC048832" in other vectors (4)
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CNY 3,784.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Grg6, 1810057E06Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208164 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATTGGTGTTGGGGCTGAAGGGGACAGTTGGCCATGTCATGTGTCTCATGAGGCCCCAATGGGCT CTGCCCAGACCACAGAGAACTCAGCTAAGGAAGAAGACAAACAGGTCCCAGAGTCTGCAGCCCTCCAGCA CCCCAAGTTCAAAAGCACCCCGGGGCCTCAGCTGCCCACCAGACGTCGGTTCCTCTCAGAGAGTGATGAG CTCCAGGACCCACAGCCGGTCTGGGACGCGGAGCCCCAGTTCTGTCAAGGCTTCCTGATCCAGGGACTCT GGGAACTTTTCATGGACAGCCGCCAAAAGAACCAACAGGAACACGGGGGTGAGGATTCCTCACAGGAAAG CAAAGATTCAGGCCTGTGTGACTTTAAACCAGAACCTCAGCCCAGGCACAGAAACAGCCTGAGTGACTCT GCCGACCCCTTCCTGATCAAGTCTCCCTCAGCTCTTCTAGATTATTACCAAGAAGACGTCAGTCGGCCCC AACCTGAGACGCAGGAATCTTCTGGAAGAGCTGATAAATTTCTGAAGCCCTTGTCCTGGGGCTCTGAAGT CTTAGAGAGCAGCTGTAATCAGCCTAGCACAGCACTCTGGCAATTAGAGAGGTTCACTGTCCCCCAAGCA CTGCAGAAGGTGCGTGTGCTGAAGCACCAGGAACTGCTATTGGTGGTAGCTGTGAGCTCCTTCACGCGGC ACGTCTTCACCTGCAGCCAGAGCGGCATCAAGGTGTGGAACCTCGTGAACCAGGTGGCCGAGGACAGAGA CCCAGAAAGCCACTTGAAATGCAGCGTCCAGGACAACAAGGTGTACCTGCGCACCTGCCTGCTGTCCTCC AACAGCAGGACCCTGTTCGCGGGTGGATACAACTTACCTGGCGTGATCGTGTGGGACCTGGCAGCCCCAT CCCTGTATGAGAAGTGCCAGCTGCCCTGCGAGGGCCTGTCCTGCCAGGCACTGGCCAACACAAAGGAAAA CATGGCCCTTGCCGGCTTCACAGATGGCACGGTCAGGATATGGGACCTACGGACTCAGGAAATAGTCAGG AACCTCAAAGGCCCTACCAATTCGGCCAGGAACCTTGTGGTCAAAGATGACAACATCTGGACAGGGGGTC TGGACGCCTGCCTGCGATGCTGGGACCTGCGGATGGCCAAGGTGTCACTGGAGCATCTGTTCCAGTCCCA GATCATGAGCCTGGCTCACAGTCCGACCGAAGACTGGCTGCTGCTGGGCCTGGCCAATGGCCAGCACTGC CTGTTCAACAGCAGGAAGAGGGACCAGGTGCTCACTGTGGACACCAAGGACAACACCATCTTAGGCCTCA AGTTCTCCCCAAATGGCAAGTGGTGGGCGAGCGTCGGTATGGGCAACTTCATCACGGTCCACAGCATGCC CACCGGAGCAAAACTGTTCCAGGTCCCCGAGGTTGGCCCTGTCAGATGCTTCGACATGACTGAGAACGGC AGGCTCATCATCACAGGATCCAGGGACTGTGCCTCGGTGTACCACATCAAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208164 protein sequence
Red=Cloning site Green=Tags(s) MSIGVGAEGDSWPCHVSHEAPMGSAQTTENSAKEEDKQVPESAALQHPKFKSTPGPQLPTRRRFLSESDE LQDPQPVWDAEPQFCQGFLIQGLWELFMDSRQKNQQEHGGEDSSQESKDSGLCDFKPEPQPRHRNSLSDS ADPFLIKSPSALLDYYQEDVSRPQPETQESSGRADKFLKPLSWGSEVLESSCNQPSTALWQLERFTVPQA LQKVRVLKHQELLLVVAVSSFTRHVFTCSQSGIKVWNLVNQVAEDRDPESHLKCSVQDNKVYLRTCLLSS NSRTLFAGGYNLPGVIVWDLAAPSLYEKCQLPCEGLSCQALANTKENMALAGFTDGTVRIWDLRTQEIVR NLKGPTNSARNLVVKDDNIWTGGLDACLRCWDLRMAKVSLEHLFQSQIMSLAHSPTEDWLLLGLANGQHC LFNSRKRDQVLTVDTKDNTILGLKFSPNGKWWASVGMGNFITVHSMPTGAKLFQVPEVGPVRCFDMTENG RLIITGSRDCASVYHIKY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC048832 |
| ORF Size | 1524 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048832, AAH48832 |
| RefSeq Size | 1897 bp |
| RefSeq ORF | 1526 bp |
| Locus ID | 114606 |
| MW | 56.8 kDa |
| Gene Summary | As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming
,Mahadevan, S;Sathappan, V;Utama, B;Lorenzo, I;Kaskar, K;Van den Veyver, IB;,
Sci Rep
,PubMed ID 28317850
[TLE6]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217953 | Tle6 (untagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029),, (10ug) |
CNY 6,940.00 |
|
| MG208164 | Tle6 (tGFP-tagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029) |
CNY 3,140.00 |
|
| MR208164L3 | Lenti ORF clone of Tle6 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029) |
CNY 4,750.00 |
|
| MR208164L4 | Lenti ORF clone of Tle6 (mGFP-tagged) - Mouse transducin-like enhancer of split 6, homolog of Drosophila E(spl) (cDNA clone MGC:55024 IMAGE:4506029) |
CNY 4,750.00 |
