United States
Germany
Japan
United Kingdom
China
Plk3 (BC031180) Mouse Tagged ORF Clone
CAT＃: MR208102
- TrueORF®
Plk3 (Myc-DDK-tagged) - Mouse polo-like kinase 3 (Drosophila) (cDNA clone MGC:37578 IMAGE:4988360)
ORF Plasmid: tGFP
"BC031180" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fnk, PRK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208102 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCCGCCGGCTTCTTGTCTCCGCGCCCCTTCCCGCGCGCGGCTGCCCCGTCCGCGCCCCCCG CCGGCCCGGGGCCGCCTGCCAATGCCTCCCCGCGATCGGAACCTGAGGTGCTGGCCGGGCCACGGGCGCC GGATCCTCCTGGTCGCCTCATCACCGACCCTCTCAGCGGCCGTACCTACACCAAGGGTCGCTTGTTGGGC AAGGGGGGCTTCGCACGCTGCTATGAAGCCACTGACACCGAGTCTGGTATAGCCTACGCGGTCAAAGTCA TCCCGCAGAGTCGCGTCGCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATAGAGTTGCACCGAGA CCTACAGCACCGCCATATCGTTCGCTTTTCACATCATTTCGAGGATGCTGACAACATATACATTTTCCTG GAGCTCTGTAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGACACACCCTGTTGGAACCAGAGGTTC GCTACTACCTGCGCCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGAGGGGTATCTTACACAGAGATCT CAAGCTGGGAAATTTTTTCATCACGGATAACATGGAACTGAAGGTGGGGGATTTTGGGCTGGCAGCTCGA TTAGAGCCCCCAGAGCAGAGGAAAAAGACCATCTGTGGCACTCCCAACTACGTGGCTCCAGAAGTGCTGC TGAGACAGGGCCATGGCCCCGAGGCAGATGTGTGGTCGCTTGGTTGTGTCATGTACACGCTCCTGTGTGG GAGCCCCCCATTTGAGACTGCTGACCTGAAGGAGACATACCGCTGCATCAAGCAGGTTCATTACACGCTA CCTGCCAGCCTCTCCCTGCCTGCCCGGCAGCTCCTGGCTGCCATCCTCCGAGCTTCACCCAGAGACCGCC CTTCCATTGAACAGATTTTACGCCATGACTTCTTTACGAAGGGCTACACCCCTGATCGGCTCCCAGTCAG CAGCTGTGTGACAGTCCCAGATCTGACACCCCCCAACCCTGCGAGGAGTCTGTTTGCCAAAGTTACCAAG AGCCTGTTTGGCAGGAAAAAGAACAAGAATAAGAACCATTCTGAGGACCAGGATAATGTCTCCTGCTTGG TGAGTGGCCTCATGCGCACATCCATCGGTCATCCAGATGTCAGGCCGGAGGCTCCTGTAGTTTCAGGCCA GGCCCCTGCCAGCTTGATAGAGACAGCTGCCGAGGACAGCTCGCCCCGTGGGACACTGGCCAGCAGTGGA GATGGGTTTGAAGAAGGTTTGACTGTGGCCACAGTGGTAGAGTCTGCCCTCTGTGCACTGAGAAACTGTG TGGCCTTCATGCCCCCAGCCGAACAGAACCCAGCCCCACTGGCACAGCCAGAGCCTCTGGTGTGGGTCAG CAAGTGGGTTGACTACTCCAACAAGTTTGGCTTTGGCTATCAGCTGTCCAGCCGCCGTGTAGCCGTGCTC TTCAATGATGGCACACACATGGCCCTGTCAGCCAATAGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208102 protein sequence
Red=Cloning site Green=Tags(s) MEPAAGFLSPRPFPRAAAPSAPPAGPGPPANASPRSEPEVLAGPRAPDPPGRLITDPLSGRTYTKGRLLG KGGFARCYEATDTESGIAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHRHIVRFSHHFEDADNIYIFL ELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGILHRDLKLGNFFITDNMELKVGDFGLAAR LEPPEQRKKTICGTPNYVAPEVLLRQGHGPEADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTL PASLSLPARQLLAAILRASPRDRPSIEQILRHDFFTKGYTPDRLPVSSCVTVPDLTPPNPARSLFAKVTK SLFGRKKNKNKNHSEDQDNVSCLVSGLMRTSIGHPDVRPEAPVVSGQAPASLIETAAEDSSPRGTLASSG DGFEEGLTVATVVESALCALRNCVAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVL FNDGTHMALSANRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031180 |
| ORF Size | 1512 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031180, AAH31180 |
| RefSeq Size | 2648 bp |
| RefSeq ORF | 1514 bp |
| Locus ID | 12795 |
| MW | 55.5 kDa |
| Gene Summary | This gene encodes a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal catalytic domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases have primarily been implicated in cell cycle regulation. In mouse, this protein that has been reported to localize to the nucleolus during interphase but is undetectable during mitosis, following nucleolus dissociation during prophase. The protein relocalizes to the nucleolus just prior to cytokinesis and peak levels are detected during G1 of interphase. This gene has been implicated in regulation of entry into S phase, with RNAi-induced depletion resulting in failure to re-enter the cell cycle. Mice deficient for this gene exhibit increased weight and tumor development at advanced age. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208102 | Plk3 (tGFP-tagged) - Mouse polo-like kinase 3 (Drosophila) (cDNA clone MGC:37578 IMAGE:4988360) |
CNY 5,510.00 |
|
| MR208102L3 | Lenti ORF clone of Plk3 (Myc-DDK-tagged) - Mouse polo-like kinase 3 (Drosophila) (cDNA clone MGC:37578 IMAGE:4988360) |
CNY 6,940.00 |
|
| MR208102L4 | Lenti ORF clone of Plk3 (mGFP-tagged) - Mouse polo-like kinase 3 (Drosophila) (cDNA clone MGC:37578 IMAGE:4988360) |
CNY 6,940.00 |
