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Arhgap22 (BC038272) Mouse Tagged ORF Clone
CAT＃: MR208068
- TrueORF®
Arhgap22 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331)
ORF Plasmid: tGFP
"BC038272" in other vectors (4)
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CNY 3,736.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC47316, RHOGAP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208068 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCCCAGCGGGATATGGAGGACTGGGTGCAGGCCATCCGCCGTGTCATCTGGGCCCCGCTGG GCAGAGGGATCTTTGGCCAGCGGCTAGAGGACACAGTGCACCATGAGCGGAAGTTTGGTCCCCGGCTGGC ACCTCTGCTGGTGGAGCAGTGTGTGGACTTCATCCGGGAGCGAGGACTCAGTGAAGAGGGCCTCTTCCGG ATGCCTGGCCAGGCCAACCTGGTGAGGGACCTGCAGGATTCCTTCGACTGTGGGGAGAAGCCGCTGTTTG ACAGGTTTCTGGATGAAGTGCAGGCCCACTCAGATGTCAATAAGATGAGTGTCCAGAACTTGGCAACTGT CTTTGGTCCTAACATTCTTCGGCCACAGATAGAGGATCCAGTAACCATCATGGAAGGCACGTCTCTGGTT CAGCATCTGATGACTGTCCTCATCCGCAAACATGGACAGCTCTTTGCTGCAACCTCCCTTGAAGAACCTG CTTCACCACATGGCACAGTAGAGTGGGGCTCTGAAGAGGTCACCAGGGATCACCGGGGTGAGCCTGGCAG CCCTGGCTTGCCCACACATAGGACTTCATCTCTGGATGGGCCAGCTGCAGCAGTGCTATCTAGAACTTCG CCGCCGCGCCTTGGCAGCCAAACTGGCCCCGCAGCCACCAGCCCTGGGAAGAAGATGCACACTTTGCCTG TCTGGAAGTCTTCTTTTCGGCAGCAGGGATCCCGGTCAGAGAGCCCCAAAGGGGTCAACTCATCCTTAGA GGTACCCATCATCTCCTCTGGTGGGAACTGGCTCATAAACGGGCTATCGTCTCTTCGCAGCCACCGTCGG GCCTCATCGGGGGACCGCCTTAAAGATACAGGCTCGGCACAGAGACTCTCCACCTATGACAATGTGCCGC CATCTAGCCAATTTTCTAGCACTGCCAGCGTGGCCAGCACGTCATGGTCTGTAGCCTCGTCCTCCCGAGA GGCCTCTGTCAGCAGCTGCACAGCCTGCCGGGCCAGCAACTCTTCTGCCTGCAGCTCCTTACACACCGAG TGGGCTCTCGAGCCCTCTCCTCTCCCCAGCAGCAGTGAGGGCCACCAGTCCCCGGACCTGGGCCACAGCC TGGATGAGCCATGTGTGGGCAGTGGCAGCAGTGAGCCCAATGACCCAGGCAGCCCCACACAGGCCCATGT TCGCCGCTGCCGGGCTCTCCAGGGCCAGGTGGCCGAGCTCAGGGCAGAGTTGTGTCAGCAGAGGACTGAG TACAAGAGGAGCCTGAAAAGCATCGAGGAGGGCAGTGCTGACCTGCGGAAGCAGATGTCTCGGTTGGAAG AGGAGCTGGACCAGGAGAGGAAGAAGTATGCCATGCTGGAGATCAAGCTCCGGAACTCGGAGCGAGCCCG GGAGGATGCGGAGCGCAGGAACCAGCTGCTGCAGAGGGAGATGGAGGAGTTCTTCTCCACCCTGGGGAGC TTGACTACAGGGACCAAGGGGTCCAGAGCCCCGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208068 protein sequence
Red=Cloning site Green=Tags(s) MASSQRDMEDWVQAIRRVIWAPLGRGIFGQRLEDTVHHERKFGPRLAPLLVEQCVDFIRERGLSEEGLFR MPGQANLVRDLQDSFDCGEKPLFDRFLDEVQAHSDVNKMSVQNLATVFGPNILRPQIEDPVTIMEGTSLV QHLMTVLIRKHGQLFAATSLEEPASPHGTVEWGSEEVTRDHRGEPGSPGLPTHRTSSLDGPAAAVLSRTS PPRLGSQTGPAATSPGKKMHTLPVWKSSFRQQGSRSESPKGVNSSLEVPIISSGGNWLINGLSSLRSHRR ASSGDRLKDTGSAQRLSTYDNVPPSSQFSSTASVASTSWSVASSSREASVSSCTACRASNSSACSSLHTE WALEPSPLPSSSEGHQSPDLGHSLDEPCVGSGSSEPNDPGSPTQAHVRRCRALQGQVAELRAELCQQRTE YKRSLKSIEEGSADLRKQMSRLEEELDQERKKYAMLEIKLRNSERAREDAERRNQLLQREMEEFFSTLGS LTTGTKGSRAPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC038272 |
| ORF Size | 1506 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC038272, AAH38272 |
| RefSeq Size | 2108 bp |
| RefSeq ORF | 1508 bp |
| Locus ID | 239027 |
| MW | 55.1 kDa |
| Gene Summary | Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218633 | Arhgap22 (untagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331), (10ug) |
CNY 6,940.00 |
|
| MG208068 | Arhgap22 (tGFP-tagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331) |
CNY 3,140.00 |
|
| MR208068L3 | Lenti ORF clone of Arhgap22 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331) |
CNY 4,750.00 |
|
| MR208068L4 | Lenti ORF clone of Arhgap22 (mGFP-tagged) - Mouse Rho GTPase activating protein 22 (cDNA clone MGC:47316 IMAGE:4237331) |
CNY 4,750.00 |
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