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Sp3 (BC027797) Mouse Tagged ORF Clone
CAT＃: MR207955
- TrueORF®
Sp3 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 3 (cDNA clone MGC:38209 IMAGE:5323205)
ORF Plasmid: tGFP
"BC027797" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D130027J01Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207955 representing BC027797
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCAGGCATTAATGCCGATGGACATTTGATAAACACAGGACAAGCTATGGATAGTTCAGACAATT CAGAAAGGACTGGTGAGCGGGTTTCTCCTGATGTTAATGAAACTAATGCTGATACAGATTTATTTGTGCC AACATCCTCTTCATCACAGTTGCCTGTTACAATAGATAGTACAGGTATATTACAGCAAAACACAAATAGC TTGACTACTACTAGTGGGCAAGTCCATTCTTCAGATCTTCAGGGAAATTATATCCAGTCGCCTGTTTCTG AAGAGACACAGGCTCAGAATATTCAGGTTTCTACAGCACAGCCTGTTGTACAACATCTACAACTTCAAGA TTCTCAGCAGCCAACCAGTCAAGCCCAAATTGTGCAAGGTATTACACCACAGACAATCCATGGCGTGCAA GCCAGTGGTCAAAATATATCACAACAGGCTTTGCAAAACCTTCAGTTGCAGCTGAATCCTGGAACCTTTT TAATTCAGGCACAGACAGTGACCCCTTCTGGACAGATAACTTGGCAAACATTTCAAGTACAAGGGGTCCA GAACTTACAGAATTTGCAAATACAAAATACTGCTGCCCAACAAATTACTTTGACGCCTGTTCAGACACTC ACGCTTGGTCAAGTCGCAGCAGGTGGAGCCTTGACTTCAACTCCAGTCAGTCTAAGCACTGGTCAGTTGC CAAATCTACAGACAGTTACAGTAAATTCTATAGATTCTACTGGCATACAGCTACATCCAGGAGAGAATGC CGACAGTCCTGCAGATATTAGGATCAAGGAAGAGGAACCTGACCCTGAAGAATGGCAGCTCAGTGGTGAC TCTACACTGAACACCAATGACCTAACACATTTACGAGTACAAGTGGTAGATGAAGAAGGGGACCAACAAC ATCAAGAAGGAAAAAGACTTCGGAGGGTAGCTTGCACCTGTCCCAACTGTAAAGAAGGTGGTGGGAGAGG TACCAATCTTGGGAAAAAGAAGCAACACATTTGTCATATACCGGGATGTGGTAAAGTCTATGGGAAGACC TCACATCTGAGAGCACACCTGCGTTGGCATTCGGGGGAGCGCCCTTTTATTTGTAACTGGATGTTCTGTG GTAAAAGATTTACACGAAGTGATGAATTACAGAGGCACAGAAGAACACATACAGGTGAGAAGAAGTTTGT TTGTCCAGAATGTTCAAAACGCTTTATGAGAAGTGACCACCTTGCCAAACATATTAAAACACATCAAAAT AAAAAAGTCATTCACTCTAGCAGTACAGTGCTCGCATCTGTGGAAGCTGGAAGAGATGATGCCTTGATTA CTGCAGGAGGAACAACACTTATCCTTGCAAATATTCAACAGGGGTCTGTGTCAGGGATAGGAACTGTCAA TACTTCTGCCACCAGCAATCAAGACATCCTTACCAACACTGAAATACCTTTACAGCTTGTCACAGTTTCT GGAAATGAGACAATGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC027797 |
| ORF Size | 1488 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027797, AAH27797 |
| RefSeq Size | 3188 bp |
| RefSeq ORF | 1490 bp |
| Locus ID | 20687 |
| MW | 116.8 kDa |
| Gene Summary | This gene product belongs to a family of Sp1 related transcription factors, which regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses transcription of numerous genes. Alternative splicing results in transcript variants encoding different isoforms, and one variant initiates translation from a non-AUG (AUA) codon. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218243 | Sp3 (untagged) - Mouse trans-acting transcription factor 3 (cDNA clone MGC:38209 IMAGE:5323205), (10ug) |
CNY 6,940.00 |
|
| MG207955 | Sp3 (tGFP-tagged) - Mouse trans-acting transcription factor 3 (cDNA clone MGC:38209 IMAGE:5323205) |
CNY 3,140.00 |
|
| MR207955L3 | Lenti ORF clone of Sp3 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 3 (cDNA clone MGC:38209 IMAGE:5323205) |
CNY 4,750.00 |
|
| MR207955L4 | Lenti ORF clone of Sp3 (mGFP-tagged) - Mouse trans-acting transcription factor 3 (cDNA clone MGC:38209 IMAGE:5323205) |
CNY 4,750.00 |
