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Gria2 (BC048248) Mouse Tagged ORF Clone
CAT＃: MR207939
- TrueORF®
Gria2 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (cDNA clone MGC:54603 IMAGE:6494013)
ORF Plasmid: tGFP
"BC048248" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GluR-B, short form |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207939 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAAAGATTATGCATATTTCTGTCCTCCTTTCTCCTGTTTTATGGGGACTGATTTTTGGTGTCTCTT CTAACAGCATACAGATAGGGGGGCTATTTCCAAGGGGCGCTGATCAAGAATACAGTGCATTTCGGGTAGG GATGGTTCAGTTTTCCACTTCGGAGTTCAGACTGACACCCCATATCGACAATTTGGAGGTAGCCAACAGT TTCGCAGTCACCAATGCTTTCTGCTCCCAGTTTTCAAGAGGCGTCTATGCGATTTTTGGGTTTTACGACA AGAAGTCTGTAAATACCATCACATCATTCTGTGGGACACTGCATGTATCCTTCATCACACCAAGCTTCCC AACAGATGGCACGCATCCATTTGTCATCCAGATGCGACCTGACCTCAAAGGAGCACTCCTTAGCTTGATT GAGTACTACCAATGGGATAAGTTCGCATACCTCTATGACAGTGACAGAGGCTTATCAACACTGCAAGCTG TGCTGGATTCTGCTGCGGAGAAGAAGTGGCAGGTGACTGCTATCAATGTGGGGAACATTAACAATGACAA GAAAGATGAGACCTACAGATCACTCTTTCAAGATCTGGAGTTAAAAAAAGAACGGCGTGTAATCCTTGAC TGCGAAAGGGATAAAGTCAATGACATTGTGGACCAGGTTATTACCATTGGAAAGCATGTTAAAGGGTACC ATTATATCATTGCAAATCTGGGATTTACTGATGGAGACCTGCTGAAAATTCAGTTTGGAGGAGCAAATGT CTCTGGATTTCAGATTGTAGACTACGACGACTCCCTGGTGTCTAAATTTATAGAAAGATGGTCAACACTC GAAGAGAAAGAATACCCTGGAGCACACACAGCGACAATTAAGTATACTTCGGCCCTGACTTATGATGCTG TCCAAGTGATGACTGAAGCATTCCGCAATCTTCGGAAGCAGAGGATTGAAATCTCCAGGAGAGGAAATGC AGGAGATTGTTTGGCCAACCCAGCTGTGCCTTGGGGACAAGGCGTGGAAATAGAAAGGGCCCTCAAGCAG GTTCAAGTTGAAGGTCTCTCTGGAAATATAAAATTTGACCAGAACGGAAAACGAATAAACTACACAATTA ACATCATGGAGCTCAAAACAAATGGACCCCGGAAGATTGGGTACTGGAGTGAAGTGGATAAAATGGTTGT CACCCTAACCGAGCTCCCCTCTGGAAATGACACATCTGGGCTTGAAAACAAAACTGTGGTTGTCACCACA ATATTGGAATCTCCATATGTTATGATGAAGAAAAATCATGAAATGCTTGAAGGGAATGAGCGTTATGAGG GCTACTGTGTTGACTTAGCTGCAGAAATTGCCAAACATTGTGGATTCAAGTACAAGCTGACTATTGTTGG GGATGGCAAGTATGGGGCCAGGGATGCAGACACCAAAATTTGGAATGGTATGGTTGGAGAACTTGTATAT GGGGTAAGTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207939 protein sequence
Red=Cloning site Green=Tags(s) MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANS FAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLI EYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILD CERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTL EEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQ VQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTT ILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVY GVSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC048248 |
| ORF Size | 1482 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC048248, AAH48248 |
| RefSeq Size | 4799 bp |
| RefSeq ORF | 1484 bp |
| Locus ID | 14800 |
| MW | 55.5 kDa |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218174 | Gria2 (untagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (cDNA clone MGC:54603 IMAGE:6494013), (10ug) |
CNY 6,940.00 |
|
| MG207939 | Gria2 (tGFP-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (cDNA clone MGC:54603 IMAGE:6494013) |
CNY 3,140.00 |
|
| MR207939L3 | Lenti ORF clone of Gria2 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (cDNA clone MGC:54603 IMAGE:6494013) |
CNY 4,750.00 |
|
| MR207939L4 | Lenti ORF clone of Gria2 (mGFP-tagged) - Mouse glutamate receptor, ionotropic, AMPA2 (alpha 2) (cDNA clone MGC:54603 IMAGE:6494013) |
CNY 4,750.00 |
