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March6 (BC059190) Mouse Tagged ORF Clone
CAT＃: MR207831
- TrueORF®
41339 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 6 (cDNA clone MGC:65370 IMAGE:3499678)
ORF Plasmid: tGFP
"BC059190" in other vectors (4)
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CNY 4,850.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 3830408G03; F830029L24Rik; mKIAA0597 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207831 representing BC059190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTCTGCACTGGTTGGTGGGAATGGTGTATGTGTTCTACTTTGCCTCCTTCATCCTGTTACTGAGAG AGGTACTTCGACCTGGTGTCTTATGGTTTCTGAGGAATTTGAACGATCCAGATTTTAATCCAGTACAGGA AATGATTCACTTGCCCATTTATAGACATCTCCGAAGATTTATTTTGTCAGTGATTGTCTTTGGCTCCATT GTCCTTCTGATGCTCTGGCTTCCTATACGTATAATTAAGAGTCTCTTGCCTAATTTTCTTCCTTACAATG TCATGCTCTACAGTGATGCTCCGGTGAGTGAGCTGTCCCTTGAGCTGCTCCTACTGCAGGTTGTCTTGCC AGCATTGCTGGAACAGGGACACACGAGGCAGTGGCTGAAGGGGCTTGTGCGTGCATGGACTGTTACTGCT GGATACTTGCTGGACCTTCATTCCTACTTACTGGGAGATCAGGAAGAAAACGAGAACAGTGCAAATCAGC AGGTCAACAATAACCAGCCTGCACGAAACAATAATGCTGTTCCTGCCGGGGAAGGTCTGCATGCAGCCCA CCAAGCCATACTCCAGCAGGGAGGACCTGTTGGCTTTCAGCCTTACCGCCGGCCTTTAAATTTCCCACTC AGGATATTTCTGCTGATTGTCTTCATGTGCATAACATTGCTAATTGCCAGCCTCATTTGCCTTACTTTAC CAGTATTTGCTGGCCGTTGGTTAATGTCATTTTGGACGGGGACTGCCAAAATCCATGAGCTGTACACAGC TGCTTGTGGTCTCTATGTGTGTTGGCTAACCATAAGGGCTGTGACGGTGCTGGTGGCCTGGATGCCCCAG GGACGAAGAGTGATTTTCCAGAAGGTTAAAGAGTGGTCCCTCATGATAATGAAGACGTTGATAGTTGCTG TGCTGCTGGCTGGGGTCGTCCCACTGCTCCTCGGGCTCCTGTTTGAGCTGGTTATTGTTGCTCCTCTGAG GGTCCCTCTTGATCAGACTCCCCTTTTCTACCCTTGGCAGGACTGGGCGCTGGGAGTGCTGCATGCCAAA ATTATCGCAGCTATAACTCTTATGGGTCCTCAGTGGTGGTTGAAAACTGTCATTGAACAGGTTTACGCAA ATGGCATTCGTAACATCGACCTTCACTATATCATCCGTAAGCTGGCAGCCCCGGTGATCTCTGTGCTCTT ACTTTCCCTGTGTGTACCGTATGTCATAGCTTCTGGCGCGGTTCCTCTACTAGGTGTTACTGCAGAGATG CAGAACTTAGTTCACCGGCGGATTTACCCATTTTTATTGATGGTCGTTGTGCTGATGGGAATCCTGTCCT TCCAGGTCCGCCAGTTTAAGCGCCTTTATGAACACATTAAAAATGACAAGTACCTTGTGGGGCAGCGCCT GGTGAACTATGAGCGCAAATCCGGCAAGCAAGGCCCGTCTACACCGCCTCCGGTGTCGTCCCAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059190 |
| ORF Size | 1467 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059190, AAH59190 |
| RefSeq Size | 4778 bp |
| RefSeq ORF | 1469 bp |
| Locus ID | 223455 |
| MW | 175.1 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that promotes 'Lys-48'-linked ubiquitination of target proteins, leading to their proteasomal degradation. Promotes ubiquitination of DIO2, leading to its degradation. Promotes ubiquitination of SQLE, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Destabilization of β Cell FIT2 by saturated fatty acids alter lipid droplet numbers and contribute to ER stress and diabetes
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 35254894
[March6]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218586 | 41339 (untagged) - Mouse membrane-associated ring finger (C3HC4) 6 (cDNA clone MGC:65370 IMAGE:3499678), (10ug) |
CNY 6,940.00 |
|
| MG207831 | 41339 (tGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 6 (cDNA clone MGC:65370 IMAGE:3499678) |
CNY 5,256.00 |
|
| MR207831L3 | Lenti ORF clone of 41339 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 6 (cDNA clone MGC:65370 IMAGE:3499678) |
CNY 6,750.00 |
|
| MR207831L4 | Lenti ORF clone of 41339 (mGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 6 (cDNA clone MGC:65370 IMAGE:3499678) |
CNY 6,750.00 |
