Pacsin2 (NM_011862) Mouse Tagged ORF Clone
CAT#: MR207795
- TrueORF®
Pacsin2 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 1
ORF Plasmid: tGFP
"NM_011862" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI197433 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207795 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTCACCTACGATGACTCTGTGGGAGTGGAAGTGTCCAGCGACAGCTTCTGGGAGGTTGGGAACT ACAAACGGACTGTGAAGCGGATTGACGATGGCCACCGCCTGTGTGGTGACCTCATGAACTGTCTGCATGA GCGGGCACGCATCGAGAAGGCGTATGCACAGCAGCTCACTGAGTGGGCCCGACGCTGGAGGCAGCTGGTA GAGAAGGGACCACAGTATGGGACCGTGGAGAAGGCCTGGATAGCTGTCATGTCTGAAGCAGAGAGGGTGA GTGAACTGCACCTGGAAGTGAAGGCATCACTGATGAATGAAGACTTTGAGAAGATCAAGAACTGGCAGAA GGAAGCCTTTCACAAGCAGATGATGGGAGGCTTCAAGGAGACCAAAGAAGCAGAGGATGGCTTTCGGAAG GCCCAGAAGCCCTGGGCCAAGAAGCTGAAAGAGGTGGAAGCGGCAAAGAAGGCGCACCACACAGCGTGCA AAGAGGAGAAGCTGGCCATCTCCCGGGAAGCCAACAGCAAGGCAGATCCATCCCTCAACCCTGAGCAGCT GAAGAAACTGCAAGACAAGATAGAAAAATGCAAACAGGATGTTCTAAAGACCAAGGACAAGTATGAGAAG TCCCTGAAGGAGCTTGATCAGACCACACCCCAGTACATGGAGAACATGGAGCAGGTGTTCGAGCAGTGCC AGCAGTTTGAAGAGAAGCGCCTGCGCTTCTTCCGGGAGGTTCTGCTGGAGGTTCAGAAGCACTTGGATCT GTCCAATGTGGCTAGCTATAAAACCATTTACCGGGAGCTGGAGCAGAGCATCAAAGCAGCAGATGCGGTA GAGGACCTGAGGTGGTTCCGGGCTAACCATGGGCCAGGCATGGCTATGAACTGGCCACAGTTTGAGGAGT GGTCTGCAGATCTGAATCGAACTCTCAGCCGGAGAGAGAAGAAGAAGGCTGTTGACGGTGTCACCCTAAC AGGGATCAACCAGACAGGTGACCAGTCTGGACAGAACAAGCCTGGCAGCAACCTTAGTGTCCCGAGCAAC CCCGCCCAGTCCACGCAGTTACAGTCCAGCTACAACCCCTTCGAGGACGAGGACGACACGGGCAGCAGCA TCAGTGAGAAGGAGGACATTAAGGCCAAAAATGTCAGCAGCTATGAGAAGACTCAGACTTACCCCACTGA CTGGTCTGATGATGAGTCTAACAACCCTTTCTCCTCCACGGATGCCAACGGGGATTCGAACCCATTTGAT GAGGACACGACCTCAGGAACAGAAGTGCGAGTTCGGGCCCTCTATGACTATGAGGGGCAGGAACATGATG AGCTGAGCTTCAAGGCTGGGGATGAACTGACCAAGATAGAGGATGAAGATGAACAGGGTTGGTGCAAGGG ACGTTTAGACAGCGGCCAGGTTGGCCTATACCCAGCCAACTATGTCGAGGCTATCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207795 protein sequence
Red=Cloning site Green=Tags(s) MSVTYDDSVGVEVSSDSFWEVGNYKRTVKRIDDGHRLCGDLMNCLHERARIEKAYAQQLTEWARRWRQLV EKGPQYGTVEKAWIAVMSEAERVSELHLEVKASLMNEDFEKIKNWQKEAFHKQMMGGFKETKEAEDGFRK AQKPWAKKLKEVEAAKKAHHTACKEEKLAISREANSKADPSLNPEQLKKLQDKIEKCKQDVLKTKDKYEK SLKELDQTTPQYMENMEQVFEQCQQFEEKRLRFFREVLLEVQKHLDLSNVASYKTIYRELEQSIKAADAV EDLRWFRANHGPGMAMNWPQFEEWSADLNRTLSRREKKKAVDGVTLTGINQTGDQSGQNKPGSNLSVPSN PAQSTQLQSSYNPFEDEDDTGSSISEKEDIKAKNVSSYEKTQTYPTDWSDDESNNPFSSTDANGDSNPFD EDTTSGTEVRVRALYDYEGQEHDELSFKAGDELTKIEDEDEQGWCKGRLDSGQVGLYPANYVEAIQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011862 |
ORF Size | 1461 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011862.1, NM_011862.2, NM_011862.3, NP_035992.1 |
RefSeq Size | 3732 bp |
RefSeq ORF | 1461 bp |
Locus ID | 23970 |
UniProt ID | Q9WVE8 |
MW | 55.8 kDa |
Gene Summary | Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216598 | Pacsin2 (untagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 1, (10ug) |
CNY 6,270.00 |
|
MG207795 | Pacsin2 (tGFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2) |
CNY 4,750.00 |
|
MR207795L3 | Lenti ORF clone of Pacsin2 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 1 |
CNY 6,180.00 |
|
MR207795L4 | Lenti ORF clone of Pacsin2 (mGFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 2 (Pacsin2), transcript variant 1 |
CNY 6,180.00 |
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