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Igh (BC028249) Mouse Tagged ORF Clone
CAT＃: MR207728
- TrueORF®
Igh (Myc-DDK-tagged) - Mouse immunoglobulin heavy chain complex (cDNA clone MGC:40652 IMAGE:3487241)
ORF Plasmid: tGFP
"BC028249" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207728 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATGGAGCTGTATCATGCTCTTCTTGGCAGCAACAGCTACAGGTGTCCACTCCCAGGTCCAACTGC AGCAGCCTGGGGCTGAGCTTGTGAAGCCTGGGGCTTCAGTAAAGCTGTCCTGCAAGGCTTCTGGCTACAC CTTCACCAGTTACTGGATGCACTGGGTGAAGCAGAGGCCTGGACGAGGCCTTGAGTGGATTGGAAGGATT GATCCTAATAGTGGTGGTACTAAGTACAATGAGAAGTTCAAGAGCAAGGCCACACTGACTGTAGACAAAC CCTCCAGCACAGCCTACATGCAGCTCAGCAGCCTGACATCTGAGGACTCTGCGGTCTATTACTGTACAAG AGAGGGGGATTACGACGCTATGGACTACTGGGGTCAAGGAACCTCAGTCACCGTCTCCTCAGAGCCTGCA AGAGAGCCCACCATCTACCCACTGACATTCCCACAAGCTCTGTCAAGTGACCCAGTGATAATCGGCTGCC TGATTCATGATTACTTCCCTTCCGGCACGATGAATGTGACCTGGGGAAAGAGTGGGAAGGATATAACCAC CGTAAACTTCCCACCTGCCCTGGCCTCTGGGGGACGGTACACCATGAGCAGCCAGTTGACCCTGCCAGCT GTCGAGTGCCCAGAAGGAGAATCCGTGAAATGTTCCGTGCAACATGACTCTAACCCCGTCCAAGAATTGA ACGTGAATTGCCCTGGTATCTGTTCTCCTCCTACTACTCCTCCTCCACCTTCCTGCCAGCCCAGCCTGTC ACTGCAGCGGCCAGCTCTTGAGGACCTGCTCCTGGGTTCAGATGCCAGCATCACATGTACTCTGAATGGC CTGAGAGATCCTGAGGGAGCTGTCTTCACCTGGGAGCCCTCCACTGGGAAGGATGCAGTGCAGAAGAAAG CTGTGCAGAATTCCTGCGGCTGCTACAGTGTGTCCAGCGTCCTGCCTGGCTGTGCTGAGCGCTGGAACAG TGGCGCATCATTCAAGTGCACAGTTACCCATCCTGAGTCTGACACCTTAACTGGCACAATTGCCAAAGTC ACAGTGAACACCTTCCCACCCCAGGTCCACCTGCTACCGCCGCCGTCGGAGGAGCTGGCCCTGAATGAGC TCGTGTCCCTGACATGCCTGGTGCGAGCTTTCAACCCTAAAGAAGTGCTGGTGCGATGGCTGCATGGAAA TGAGGAGCTGTCCCCAGAAAGCTACCTAGTGTTTGAGCCCCTAAAGGAGCCAGGCGAGGGAGCCACCACC TACCTGGTGACAAGCGTGTTGCGTGTATCAGCTGAAATCTGGAAACAGGGTGACCAGTACTCCTGCATGG TGGGCCACGAGGCCTTGCCCATGAACTTCACCCAGAAGACCATCGACCGTCTGTCGGGTAAACCCACCAA TGTCAGCGTGTCTGTGATCATGTCAGAGGGAGATGGCATCTGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207728 protein sequence
Red=Cloning site Green=Tags(s) MGWSCIMLFLAATATGVHSQVQLQQPGAELVKPGASVKLSCKASGYTFTSYWMHWVKQRPGRGLEWIGRI DPNSGGTKYNEKFKSKATLTVDKPSSTAYMQLSSLTSEDSAVYYCTREGDYDAMDYWGQGTSVTVSSEPA REPTIYPLTFPQALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGRYTMSSQLTLPA VECPEGESVKCSVQHDSNPVQELNVNCPGICSPPTTPPPPSCQPSLSLQRPALEDLLLGSDASITCTLNG LRDPEGAVFTWEPSTGKDAVQKKAVQNSCGCYSVSSVLPGCAERWNSGASFKCTVTHPESDTLTGTIAKV TVNTFPPQVHLLPPPSEELALNELVSLTCLVRAFNPKEVLVRWLHGNEELSPESYLVFEPLKEPGEGATT YLVTSVLRVSAEIWKQGDQYSCMVGHEALPMNFTQKTIDRLSGKPTNVSVSVIMSEGDGICY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC028249 |
| ORF Size | 1446 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC028249 |
| RefSeq Size | 1547 bp |
| RefSeq ORF | 1448 bp |
| Locus ID | 111507 |
| MW | 52.1 kDa |
| Gene Summary | Summary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. The RefSeq represents the IGH locus from C57BL/6. Several V and D segments in C57BL/6 are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207728 | Igh (tGFP-tagged) - Mouse immunoglobulin heavy chain complex (cDNA clone MGC:40652 IMAGE:3487241) |
CNY 3,040.00 |
|
| MR207728L3 | Lenti ORF clone of Igh (Myc-DDK-tagged) - Mouse immunoglobulin heavy chain complex (cDNA clone MGC:40652 IMAGE:3487241) |
CNY 4,660.00 |
|
| MR207728L4 | Lenti ORF clone of Igh (mGFP-tagged) - Mouse immunoglobulin heavy chain complex (cDNA clone MGC:40652 IMAGE:3487241) |
CNY 4,660.00 |
