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Polr1e (BC034783) Mouse Tagged ORF Clone
CAT＃: MR207725
- TrueORF®
Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)
ORF Plasmid: tGFP
"BC034783" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 53kDa; AU042259; D030019D19Rik; Paf53; Praf1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207725 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCTGGAGTCTCCTGGGATGGATGACCAGGCTGGAGACACGGAGACAGAGGCGCTACAGAGTG CAAGGTGGCTGTACTGTGGAGAGCCCGACGACAGGCAGAAAGCTGTGCTGGTCCAGTTCTCCAATGGGAA GCTGCAGAATCCGGGCGACATGCGCTTCACCTTGTACAACAGCACCGACTTAGTAAACCCCAGGCAGAGG AGTCACCGGATCGTGGCAGCTGAAACAGACAGGCTCTCCTATGTCGGAAACAATTTTGGGACCGGTGCCC TCAAATGCAATGCTCTTTGCAGACACTTTGTGGGAATTCTGAATAAGACCTCTGGCCAGATGGAAGTCTA TGATGCCGAACTGTTCAACATGCAGCCGCTGTTTGCTGGAATGGGAACTGAAGTCATCAAACTTGGTGGC CAACACCTTTACCTGCTGGCCTTCTGCCAACCCTCCAAGAACCTGGCTGAGGCAGGTGACCTGCTGCTGA GCCGACACCGGCAAGGCCATTGCATTGCAGTCCTGCTGGATGACGATGCTATTGAGCGAGAGCCTCCTCT GGAGAATCAGAACAAGACTTTCAGGGACAAGTTGGATTCTTGCATTGAAGCCTTTGGAAGCACCAAGCAG AAGCGGTCCCTAAACTCCAGGCGGATGAACAAAGTTGGCAGCGAGTCTCTGAATCTCAGTGTGGCGAAAG CCGCGGAGAGTATCATTGATACAAAGGGTGTGAATGCGCTGGTCAGTGATGCCATGCAGGATGACTTACA ATATGACGCCCTCTACCTTCCTCCCTGCTACGCTGACGCAGCCAAGCCGGAAGACGTGTATAGATTTGAA GACATTCTGTCCCCGGCCGAGTATGATGCTCTTGAGAGCCCATCCGAAGCCTTCAGAAAGGTCACATCGG AAGACATACTGAAGATGATTGAAGAGAACAGCCACTGCTCCTACGTCATAGAAATGCTGAAGTCTCTGCC CATAGATGAGGTCCACCGGAACCGGCAAGCCCGGAGCATCTGGTTCCTAGACGCCCTCATCAGATTCCGA GCTCAGAAAGTGATAAAGGGGAAAAGAGCCTTAGGGCCTGGAATCCCGCACATTATCAACACCAAGCTGC TGAAACAGTTCACCTGCTTGACCTACAACAACGGCAGGTTACAGAACCTAATTTCAAGTTCTATGAGGGC CAAGATCACTTCGTATGCGATCATACTTGCCCTGCACATAAATAACTTCCAGGTTGACCTGACAGCGTTG CAGAAGGACTTGAAGCTGAGTGAGAAAAGGATTATTGAGATAGCGAAAGCCATGAGGCTGAAGATCTCCA AACAAAAGGTGTCCCTGGCAGATGGCAGGGAAGAGAGTCACAGACTGGGCACTCTGTCTGTCCCACTGCC TCCAGCCCAGAACTCTGATCGACAGTCAAAGCGGAGGAAAATGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207725 protein sequence
Red=Cloning site Green=Tags(s) MAALESPGMDDQAGDTETEALQSARWLYCGEPDDRQKAVLVQFSNGKLQNPGDMRFTLYNSTDLVNPRQR SHRIVAAETDRLSYVGNNFGTGALKCNALCRHFVGILNKTSGQMEVYDAELFNMQPLFAGMGTEVIKLGG QHLYLLAFCQPSKNLAEAGDLLLSRHRQGHCIAVLLDDDAIEREPPLENQNKTFRDKLDSCIEAFGSTKQ KRSLNSRRMNKVGSESLNLSVAKAAESIIDTKGVNALVSDAMQDDLQYDALYLPPCYADAAKPEDVYRFE DILSPAEYDALESPSEAFRKVTSEDILKMIEENSHCSYVIEMLKSLPIDEVHRNRQARSIWFLDALIRFR AQKVIKGKRALGPGIPHIINTKLLKQFTCLTYNNGRLQNLISSSMRAKITSYAIILALHINNFQVDLTAL QKDLKLSEKRIIEIAKAMRLKISKQKVSLADGREESHRLGTLSVPLPPAQNSDRQSKRRKMN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC034783 |
| ORF Size | 1446 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC034783, AAH34783 |
| RefSeq Size | 1757 bp |
| RefSeq ORF | 1448 bp |
| Locus ID | 64424 |
| MW | 54.1 kDa |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217814 | Polr1e (untagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372), (10ug) |
CNY 6,940.00 |
|
| MG207725 | Polr1e (tGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 3,040.00 |
|
| MR207725L3 | Lenti ORF clone of Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 4,660.00 |
|
| MR207725L4 | Lenti ORF clone of Polr1e (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372) |
CNY 4,660.00 |
