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F10 (NM_007972) Mouse Tagged ORF Clone
CAT＃: MR207705
- TrueORF®
F10 (Myc-DDK-tagged) - Mouse coagulation factor X (F10)
ORF Plasmid: tGFP
"NM_007972" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI1947; Cf10; fX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207705 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAGCCCGGTGCAACTCAGCCTGCTCTGTGTTGTCCTGGCCAGCCTCCTGCTCCCTGGGAAAGGTG TGTTTATTAACCGGGAACGTGCCAACAATGTCCTGGCGAGGACTCGGAGGGCAAACTCATTTTTTGAAGA GTTCAAGAAAGGAAATCTGGAAAGAGAGTGTATGGAAGAAATTTGTTCTTATGAAGAGGTCCGTGAAATC TTCGAGGACGACGAGAAGACGAAAGAATACTGGACCAAATATAAAGACGGCGACCAGTGTGAAAGCAGCC CTTGCCAGAACCAAGGAGCGTGTCGAGATGGCATCGGGGGTTACACGTGCACCTGCTCGGAGGGATTTGA AGGAAAAAACTGTGAGCTCTTTGTTCGGAAACTCTGCCGCCTAGACAACGGAGACTGTGACCAGTTCTGC AGAGAAGAGCAGAACTCAGTGGTGTGCTCCTGCGCCAGCGGTTACTTCCTGGGTAATGATGGCAAGTCCT GCATCTCCACAGCTCCCTTCCCCTGTGGAAAAATCACTACAGGACGTAGGAAGAGGTCTGTGGCCCTAAA CACCAGCGACAGTGAGCTTGACCTTGAAGACGCCCTGCTTGATGAGGATTTCCTGTCCCCTACGGAGAAT CCTATTGAACTGCTCAACCTCAACGAGACACAGCCTGAGAGGAGCAGCGATGACCTTGTTCGGATTGTGG GTGGCCGGGAATGCAAGGATGGAGAATGTCCCTGGCAGGCTCTGCTCGTTAACGAAGACAATGAAGGGTT CTGTGGGGGCACCATCTTGAATGAGTTCTACATCCTCACTGCTGCCCACTGTCTCCATCAGGCCAGGCGA TTCAAGGTGAGGGTAGGTGATCGGAACACAGAGAAGGAAGAAGGCAACGAGATGGTGCACGAGGTGGACG TGGTCATTAAGCACAACAAGTTTCAGAGGGACACCTACGACTATGATATCGCCGTGCTGAGGCTGAAGAC TCCCATCACGTTCCGGATGAACGTGGCCCCTGCCTGCCTGCCTCAGAAAGACTGGGCCGAGTCCACACTG ATGACACAGAAGACGGGCATCGTGAGCGGGTTTGGACGCACGCATGAGAAGGGCCGCCAGTCGAACATCC TGAAGATGCTGGAGGTACCCTACGTGGATCGCAACACCTGCAAGCTCTCCACCAGCTTCAGCATCACACA GAATATGTTCTGTGCGGGCTATGAGGCCAAGTTAGAGGATGCCTGCCAGGGGGACAGTGGTGGCCCCCAT GTCACACGGTTCAAAAACACCTACTATGTGACCGGCATTGTCAGCTGGGGAGAGGGGTGTGCAAGGAAAG GGAAATATGGCATCTACACAAAGGTCACGACCTTCCTCAAGTGGATTGACAGGTCCATGAAAGCCAGGGT GGGACCCACAGCCGAGACCCCAAGGACAGCAGGTCCGCCCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207705 protein sequence
Red=Cloning site Green=Tags(s) MGSPVQLSLLCVVLASLLLPGKGVFINRERANNVLARTRRANSFFEEFKKGNLERECMEEICSYEEVREI FEDDEKTKEYWTKYKDGDQCESSPCQNQGACRDGIGGYTCTCSEGFEGKNCELFVRKLCRLDNGDCDQFC REEQNSVVCSCASGYFLGNDGKSCISTAPFPCGKITTGRRKRSVALNTSDSELDLEDALLDEDFLSPTEN PIELLNLNETQPERSSDDLVRIVGGRECKDGECPWQALLVNEDNEGFCGGTILNEFYILTAAHCLHQARR FKVRVGDRNTEKEEGNEMVHEVDVVIKHNKFQRDTYDYDIAVLRLKTPITFRMNVAPACLPQKDWAESTL MTQKTGIVSGFGRTHEKGRQSNILKMLEVPYVDRNTCKLSTSFSITQNMFCAGYEAKLEDACQGDSGGPH VTRFKNTYYVTGIVSWGEGCARKGKYGIYTKVTTFLKWIDRSMKARVGPTAETPRTAGPPN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007972 |
| ORF Size | 1446 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007972.2 |
| RefSeq Size | 2503 bp |
| RefSeq ORF | 1446 bp |
| Locus ID | 14058 |
| UniProt ID | O88947 |
| MW | 54 kDa |
| Gene Summary | This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216596 | F10 (untagged) - Mouse coagulation factor X (F10), (10ug) |
CNY 6,270.00 |
|
| MG207705 | F10 (tGFP-tagged) - Mouse coagulation factor X (F10) |
CNY 4,750.00 |
|
| MR207705L3 | Lenti ORF clone of F10 (Myc-DDK-tagged) - Mouse coagulation factor X (F10) |
CNY 6,180.00 |
|
| MR207705L4 | Lenti ORF clone of F10 (mGFP-tagged) - Mouse coagulation factor X (F10) |
CNY 6,180.00 |
