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Mapkapk5 (NM_010765) Mouse Tagged ORF Clone
CAT＃: MR207584
- TrueORF®
Mapkapk5 (Myc-DDK-tagged) - Mouse MAP kinase-activated protein kinase 5 (Mapkapk5)
ORF Plasmid: tGFP
"NM_010765" in other vectors (3)
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CNY 4,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MK5; PRAK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207584 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAGGACAGCGACATGGAGAAAGCCATCAAGGAGACCTCCATTTTAGAAGAATATAGTATCAATT GGACTCAGAAACTGGGAGCCGGAATTAGTGGTCCAGTTAGAGTCTGTGTGAAGAAATCCACTCAAGAACG GTTTGCACTGAAAATTCTTCTTGATCGTCCAAAAGCTAGAAATGAGGTGCGCCTGCACATGATGTGTGCC ACACACCCCAACATAGTTCAGATTATTGAAGTGTTTGCTAACAGTGTACAGTTCCCTCATGAGTCCAGCC CCAGGGCTCGACTCTTAATTGTAATGGAGATGATGGAAGGGGGAGAGCTATTTCACAGAATCAGCCAGCA CCGGCACTTTACAGAGAAGCAAGCCAGCCAAGTAACAAAGCAGATAGCCCTGGCTCTACAGCACTGTCAC TTGCTAAACATTGCGCACAGAGACCTCAAGCCTGAAAATCTGCTTTTCAAGGATAACTCTCTGGACGCCC CTGTGAAATTATGTGACTTTGGGTTTGCTAAAGTTGACCAAGGTGATTTGATGACACCCCAGTTTACCCC TTACTATGTAGCACCTCAGGTACTGGAAGCGCAGAGACGGCACCAGAAGGAGAAGTCTGGCATCATACCT ACCTCGCCAACACCCTACACTTACAACAAGAGCTGTGACTTGTGGTCCCTAGGGGTGATAATTTATGTGA TGCTGTGCGGATATCCTCCTTTTTACTCCAAACACCATAGTCGGACTATCCCAAAGGATATGCGGAAAAA GATCATGACAGGAAGTTTCGAGTTCCCAGAAGAAGAGTGGAGCCAGATCTCAGAGATGGCTAAAGATGTT GTGAGGAAGCTTCTGAAGGTCAAACCAGAGGAAAGACTCACAATCGAGGGAGTGTTGGACCATCCCTGGC TCAACTCGACAGAGGCCCTGGATAATGTGCTACCCTCTGCCCAGCTGATGATGGATAAGGCGGTGGTTGC GGGGATCCAGCAGGCGCACGCCGAGCAGCTGGCAAACATGAGGATCCAGGACCTCAAGGTCAGCCTCAAA CCCCTGCACTCTGTCAACAACCCCATTCTCAGGAAGAGGAAGCTGCTGGGCACCAAGCCAAAGGACGGTA TTTATATACACGACCATGAGAATGGAACTGAGGACTCAAATGTTGCCTTGGAAAAGCTTCGAGATGTCAT TGCCCAGTGTATCCTCCCCCAGGCTGGTAAAGGAGAGAATGAAGATGAGAAGCTGAATGAGGTAATGCAG GAGGCCTGGAAGTACAACCGCGAATGCAAGCTCCTGAGGGATGCTCTGCAGAGTTTTAGCTGGAATGGCC GTGGATTCACAGATAAAGTTGACCGATTGAAGCTGGCAGAGGTGGTAAAGCAGGTGATCGAAGAGCAGAC CCTTCCCCACGAGCCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207584 protein sequence
Red=Cloning site Green=Tags(s) MSEDSDMEKAIKETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALQHCH LLNIAHRDLKPENLLFKDNSLDAPVKLCDFGFAKVDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIP TSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTIPKDMRKKIMTGSFEFPEEEWSQISEMAKDV VRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANMRIQDLKVSLK PLHSVNNPILRKRKLLGTKPKDGIYIHDHENGTEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQ EAWKYNRECKLLRDALQSFSWNGRGFTDKVDRLKLAEVVKQVIEEQTLPHEPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010765 |
| ORF Size | 1422 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010765.2 |
| RefSeq Size | 2229 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 17165 |
| UniProt ID | O54992 |
| MW | 54.2 kDa |
| Gene Summary | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3' UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207584 | Mapkapk5 (tGFP-tagged) - Mouse MAP kinase-activated protein kinase 5 (Mapkapk5) |
CNY 5,230.00 |
|
| MR207584L3 | Lenti ORF clone of Mapkapk5 (Myc-DDK-tagged) - Mouse MAP kinase-activated protein kinase 5 (Mapkapk5) |
CNY 6,650.00 |
|
| MR207584L4 | Lenti ORF clone of Mapkapk5 (mGFP-tagged) - Mouse MAP kinase-activated protein kinase 5 (Mapkapk5) |
CNY 6,650.00 |
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