Hhat (BC008159) Mouse Tagged ORF Clone

CAT#: MR207568

  • TrueORF®

Hhat (Myc-DDK-tagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716)

ORF Plasmid: DDK tGFP



  "BC008159" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC11697, Skn
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207568 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCCGGGTGGGAACTGACTCTCTGCCTGCTTGTTTCCTTGGGTTTCCACTTCCGCTCTTTCTATG
AAGTTTACAAAGTCTCCAGAGAGCATGAAGAGGAACTGGATCAAGAATTTGAGCTGGAGATGGACACTCT
ATTTGGAGGACTGAAGAAGGACCCGACTGATTTTGAGTGGAACTTCTGGATGGAATGGGGGAAGCGGAGG
CTGGTGTGGCTCTTCATTGGCCATATGGCTGTGTCTCAGTTGGCCACGTTACTCACAAAGAAGCACAGAC
CCTGGATTGTCATGGTCTATGGAATGTGGGCCTGCTGGTGTGTGCTGGGGGCGCCAGGCGTGGTCATGGT
CCTTCTCCACAGCACCATCGCGTTCTGCGTGGCTCAGTTCCGATCCGTGCTCCTGTCCTGGCTGTGTTCT
CTCCTTCTGCTCTCCACCCTAAGGCTGCAAAGTGTGGAGGAAGTTAAGAGAAGGTGGTACAAGACAGAGA
ACGAGTATTACCTGCTCCAGTTTACGCTCACTGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGAGCT
GTGCCGGCAGCCTCCGTCTGCACAACCTACTCCATCTGCGCAAGGTGCCTCCCATTCCTACCCGTGGCTG
CTGACCTACGTCTTCTATTATCCCGTCTTCCACAACGGGCCCATCCTTAACTTCCCGGAGTTCTTCAGAC
AGATGCAGCAGCCAGAGCTCAACTCTCTGCAGCACAGCCTCTGCATTGTAGCCAAGGGCCTGGGTCGTCT
GCTCTGCTGGTGGTGGCTGGCTGAACTGATGGTACACCTCATGTACATGCATGCCCTCTACAGCAGCGCC
CCCCTCCTGGAGAGCGTTTCCTGCTGGACCTTAGGAGGACTGGCGCTGGCCCAAGTGCTCTTCTTCTATG
TGAAGTACCTGGTGCTCTTTGGTGTCCCGGCTCTGCTCATGCGCCTGGATGGACTCACGCCACCACCTCT
TCCCCGCTGCGTGAGCACCATGTTCAGTTTTACCGGGATGTGGAGGTATTTTGATGTTGGACTACACAAT
TTCTTAATCAGGTATGTGTACATCCCACTGGGCGGGTCCCAGCATGGCCTGTTGGGGACACTGCTTTCTA
CTGCAACAACGTTTGCGTTCGTGAGCTACTGGCATGGTAGCTATGAGGACCTCTGGTGCTGGGCAGCGCT
CAACTGGCTGGGAGTCACTGTGGAGAGTGGGGTCCGGAGGCTGCTAGAGACACCCTGTGTCCGGGAGACT
TTGGCCCGGCACCTCTCCCCACAAGCCCACCATCGACTGCACGCTCTGCTTGCCGCCTGCTCTACCTCCA
TGCTCATCCTCTTCAACCTGGTGTTCCTCGGGGGAATTCAAGTTGGGAAAACCTACTGGAACAGGATCTT
CCTGCAAGCAGCCTTCAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207568 protein sequence
Red=Cloning site Green=Tags(s)

MLPGWELTLCLLVSLGFHFRSFYEVYKVSREHEEELDQEFELEMDTLFGGLKKDPTDFEWNFWMEWGKRR
LVWLFIGHMAVSQLATLLTKKHRPWIVMVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCS
LLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWL
LTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSA
PLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHN
FLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRET
LARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQAAFR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC008159
ORF Size 1419 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC008159, AAH08159
RefSeq Size 1832 bp
RefSeq ORF 1421 bp
Locus ID 226861
MW 54.8 kDa
Gene Summary Catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling during limb development. May bind GTP.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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