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Hhat (BC008159) Mouse Tagged ORF Clone
CAT＃: MR207568
- TrueORF®
Hhat (Myc-DDK-tagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716)
ORF Plasmid: tGFP
"BC008159" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC11697, Skn |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207568 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCCCGGGTGGGAACTGACTCTCTGCCTGCTTGTTTCCTTGGGTTTCCACTTCCGCTCTTTCTATG AAGTTTACAAAGTCTCCAGAGAGCATGAAGAGGAACTGGATCAAGAATTTGAGCTGGAGATGGACACTCT ATTTGGAGGACTGAAGAAGGACCCGACTGATTTTGAGTGGAACTTCTGGATGGAATGGGGGAAGCGGAGG CTGGTGTGGCTCTTCATTGGCCATATGGCTGTGTCTCAGTTGGCCACGTTACTCACAAAGAAGCACAGAC CCTGGATTGTCATGGTCTATGGAATGTGGGCCTGCTGGTGTGTGCTGGGGGCGCCAGGCGTGGTCATGGT CCTTCTCCACAGCACCATCGCGTTCTGCGTGGCTCAGTTCCGATCCGTGCTCCTGTCCTGGCTGTGTTCT CTCCTTCTGCTCTCCACCCTAAGGCTGCAAAGTGTGGAGGAAGTTAAGAGAAGGTGGTACAAGACAGAGA ACGAGTATTACCTGCTCCAGTTTACGCTCACTGTTCGCTGCCTGTACTACACCAGCTTCAGCCTGGAGCT GTGCCGGCAGCCTCCGTCTGCACAACCTACTCCATCTGCGCAAGGTGCCTCCCATTCCTACCCGTGGCTG CTGACCTACGTCTTCTATTATCCCGTCTTCCACAACGGGCCCATCCTTAACTTCCCGGAGTTCTTCAGAC AGATGCAGCAGCCAGAGCTCAACTCTCTGCAGCACAGCCTCTGCATTGTAGCCAAGGGCCTGGGTCGTCT GCTCTGCTGGTGGTGGCTGGCTGAACTGATGGTACACCTCATGTACATGCATGCCCTCTACAGCAGCGCC CCCCTCCTGGAGAGCGTTTCCTGCTGGACCTTAGGAGGACTGGCGCTGGCCCAAGTGCTCTTCTTCTATG TGAAGTACCTGGTGCTCTTTGGTGTCCCGGCTCTGCTCATGCGCCTGGATGGACTCACGCCACCACCTCT TCCCCGCTGCGTGAGCACCATGTTCAGTTTTACCGGGATGTGGAGGTATTTTGATGTTGGACTACACAAT TTCTTAATCAGGTATGTGTACATCCCACTGGGCGGGTCCCAGCATGGCCTGTTGGGGACACTGCTTTCTA CTGCAACAACGTTTGCGTTCGTGAGCTACTGGCATGGTAGCTATGAGGACCTCTGGTGCTGGGCAGCGCT CAACTGGCTGGGAGTCACTGTGGAGAGTGGGGTCCGGAGGCTGCTAGAGACACCCTGTGTCCGGGAGACT TTGGCCCGGCACCTCTCCCCACAAGCCCACCATCGACTGCACGCTCTGCTTGCCGCCTGCTCTACCTCCA TGCTCATCCTCTTCAACCTGGTGTTCCTCGGGGGAATTCAAGTTGGGAAAACCTACTGGAACAGGATCTT CCTGCAAGCAGCCTTCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207568 protein sequence
Red=Cloning site Green=Tags(s) MLPGWELTLCLLVSLGFHFRSFYEVYKVSREHEEELDQEFELEMDTLFGGLKKDPTDFEWNFWMEWGKRR LVWLFIGHMAVSQLATLLTKKHRPWIVMVYGMWACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCS LLLLSTLRLQSVEEVKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWL LTYVFYYPVFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHALYSSA PLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTMFSFTGMWRYFDVGLHN FLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLWCWAALNWLGVTVESGVRRLLETPCVRET LARHLSPQAHHRLHALLAACSTSMLILFNLVFLGGIQVGKTYWNRIFLQAAFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC008159 |
| ORF Size | 1419 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC008159, AAH08159 |
| RefSeq Size | 1832 bp |
| RefSeq ORF | 1421 bp |
| Locus ID | 226861 |
| MW | 54.8 kDa |
| Gene Summary | Catalyzes N-terminal palmitoylation of SHH; which is required for SHH signaling during limb development. May bind GTP.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218004 | Hhat (untagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716), (10ug) |
CNY 6,940.00 |
|
| MG207568 | Hhat (tGFP-tagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716) |
CNY 2,950.00 |
|
| MR207568L3 | Lenti ORF clone of Hhat (Myc-DDK-tagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716) |
CNY 4,560.00 |
|
| MR207568L4 | Lenti ORF clone of Hhat (mGFP-tagged) - Mouse hedgehog acyltransferase (cDNA clone MGC:11697 IMAGE:3963716) |
CNY 4,560.00 |
