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Sucla2 (NM_011506) Mouse Tagged ORF Clone
CAT＃: MR207397
- TrueORF®
Sucla2 (Myc-DDK-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (Sucla2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_011506" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930547K18Rik; A-SCS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207397 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTCCATGTTCTACGGCCGGCAGTTGGCCGCGGCCGCCCTGAGGAGCCACCGGCCGCAGACTA CGCTGCGAGCCGCCGCCCAGGTTCTGGGAAATTCTGGCTTGTTTAACAAGCATGGACTACAAGTGCAGCA GCAGCAGCAGAGGACCCTTTCGCTGCATGAATACTTGAGTATGGAGTTATTGCAGGAAGCGGGTGTCTCT GTTCCCAAAGGCTTCGTGGCAAAGTCATCCGATGAAGCTTACGCAATTGCCAAAAAATTAGGATCAAAGG ATGTTGTGATAAAGGCACAGGTTTTGGCTGGCGGCAGAGGAAAAGGAACATTTACAAGTGGCCTCAAAGG AGGAGTGAAGATTGTTTTCTCTCCAGAAGAAGCAAAAGCTGTTTCCTCACAGATGATTGGGCAAAAATTG ATTACCAAGCAAACTGGAGAAAAGGGCAGAATATGCAATCAAGTTTTGGTCTGTGAGCGCAAATATCCCA GGAGAGAATACTACTTTGCAATAACAATGGAGAGGTCATTTCAAGGTCCTGTACTGATAGGAAGTGCACA AGGTGGTGTTAACATTGAAGATGTGGCTGCCGAGAACCCTGAAGCCATTGTAAAGGAGCCTATTGATATA GTGGAGGGCATCAAAAAGGAACAAGCCGTGACACTTGCACAGAAGATGGGATTTCCTTCTAACATTGTGG ACTCTGCAGCAGAAAACATGATCAAGCTTTATAATCTTTTTCTGAAGTATGATGCAACTATGGTAGAAAT AAACCCAATGGTAGAAGATTCTGACGGAAAGGTGTTGTGCATGGATGCAAAGATCAATTTTGATTCAAAT TCAGCCTACCGCCAGAAGAAGATCTTTGATCTTCAGGACTGGAGTCAGGAAGACGAAAGGGACAAAGAAG CAGCTAATGCAGATATTAACTACATCGGCCTCGATGGGAGCATAGGATGTCTAGTAAATGGTGCTGGCTT GGCTATGGCCACAATGGATATAATAAAACTACACGGAGGGACTCCAGCCAACTTTCTTGATGTTGGTGGA GGCGCCACAGTCCAGCAAGTAACAGAAGCGTTTAAACTCATCACTTCAGATAAAAAGGTGCAGGCTATTC TGGTCAACATTTTTGGAGGAATCATGAGATGTGATGTTATTGCACAAGGAATAGTCATGGCAGTAAAGGA TTTAGAAATCAGAATACCTGTTGTGGTGCGGTTACAAGGTACACGAGTTGATGATGCTAAGGCACTAATA GCAGACAGTGGTCTTAAAATCCTTGCTTGTGATGATTTGGATGAAGCTGCTAAAATGGTTGTAAAGCTCT CTGAAATAGTGACCTTAGCCAAAGAAGCCCATGTGGATGTGAAGTTTCAATTGCCAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207397 protein sequence
Red=Cloning site Green=Tags(s) MAASMFYGRQLAAAALRSHRPQTTLRAAAQVLGNSGLFNKHGLQVQQQQQRTLSLHEYLSMELLQEAGVS VPKGFVAKSSDEAYAIAKKLGSKDVVIKAQVLAGGRGKGTFTSGLKGGVKIVFSPEEAKAVSSQMIGQKL ITKQTGEKGRICNQVLVCERKYPRREYYFAITMERSFQGPVLIGSAQGGVNIEDVAAENPEAIVKEPIDI VEGIKKEQAVTLAQKMGFPSNIVDSAAENMIKLYNLFLKYDATMVEINPMVEDSDGKVLCMDAKINFDSN SAYRQKKIFDLQDWSQEDERDKEAANADINYIGLDGSIGCLVNGAGLAMATMDIIKLHGGTPANFLDVGG GATVQQVTEAFKLITSDKKVQAILVNIFGGIMRCDVIAQGIVMAVKDLEIRIPVVVRLQGTRVDDAKALI ADSGLKILACDDLDEAAKMVVKLSEIVTLAKEAHVDVKFQLPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011506 |
| ORF Size | 1392 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011506.4 |
| RefSeq Size | 2108 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 20916 |
| UniProt ID | Q9Z2I9 |
| MW | 50.1 kDa |
| Gene Summary | ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216238 | Sucla2 (untagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (Sucla2), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 6,080.00 |
|
| MG207397 | Sucla2 (tGFP-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (Sucla2) |
CNY 3,710.00 |
|
| MR207397L3 | Lenti ORF clone of Sucla2 (Myc-DDK-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (Sucla2), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
|
| MR207397L4 | Lenti ORF clone of Sucla2 (mGFP-tagged) - Mouse succinate-Coenzyme A ligase, ADP-forming, beta subunit (Sucla2), nuclear gene encoding mitochondrial protein |
CNY 5,230.00 |
