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Fgd2 (BC021845) Mouse Tagged ORF Clone
CAT＃: MR207373
- TrueORF®
Fgd2 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697)
ORF Plasmid: tGFP
"BC021845" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Tcd-2; Tcd2; tcs-2; tcs2; ZFYVE4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207373 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAGCGAGTACGTGAAGAACTTTGAGCGGGCCGCGGAACTGCTGGCCACGTGGATGGACAAGTCTC AGCCCTTCCAGGAGGTGGTCACCCGCATCCAGTGCAGCGAGGCCTCCAGCAGCCTGACCCTGCAGCACCA CATGTTGGAGCCTGTGCAAAGAATCCCGCGGTACGAACTGCTGCTCAAGGAATATGTGCAGAAGCTGCCA GCCCAGGCCCCAGACCTCGAAGATGCCCAGAGAGCACTGGACATGATCTTCTCAGCTGCACAGCACTCCA ATGCAGCCATTGCAGAGATGGAGCGGCTGCAGGGCCTGTGGGATGTGTACCAGCGCCTGGGCTTGGAGGA TGACATCGTGGACCCCTCCAACACCCTGCTCCGAGAGGGCCCTGTTCTCAAGATCTCTTTCCGCCGCAGC GACCCAATGGAACGCTACCTGGTTTTGTTCAACAACATGCTTCTGTATTGTGTACCCCGAGTCCTCCAAG TGGGTGCCCAGTTCCAGGTGCGGACTCGCATCGATGTGGCCGGCATGAAGGTGCGGGAGCTGACCGATGC TGAGTTCCCACACTCCTTCCTGGTGTCCGGAAAGCAGCGCACACTGGAGCTGCAGGCCCGGTCCCGAGAT GAAATGGTTTCCTGGATGCAGGCCTGCCAGGCAGCCATTGACCAGGTTGAGAAGCGGAGTGAGACCTTCA AGGCTGCTGTCCAGGGACCTCAGGGGGACACGCAGGAGCCCAAGCCACAGGTAGAGGAGCTGGGTCTCCG AGCGCCTCAGTGGGTCCGGGACAAGATGGTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAATGCCCTG ACTCGTCGGCGCCACCACTGCCGGGCTTGCGGCTATGTAGTGTGTGCCAAGTGCTCTGACTACCGTGCGG AGCTGAAATATGACAGCAACAGGCCCAACCGAGTCTGCCTGACCTGCTACACATTTCTCACTGGAAACGT ACTCCCTCAAGGCAAGGAGGACAAGAGGCGGGGCATCTTGGAGAAAGAGGCCTCAGCAGCACCCGAGCAG AGTCTGGTGTGCAGCTTCCTGCAGCTCATAGGAGACAAGTGCAGCAGGAGCCTTCCCCGGAGTTGGTGTG TGATCCCCCGGGATGACCCCCTTGTCCTGTATGTCTATGCAGCCCCCCAGGACACAAAGGCTCACACCTC CATCCCCCTACTGGGCTATCAGGTGATTTCAGGGCCCCAGGGGGACCCTCGGGTTTTCCAGCTGCAACAG TCAGGCCAGCAGTACACCTTCAAGGCCGAGTCTGTGGAGCTGCAGGGCCGCTGGGTGACAGCTATCAAGC GTGCGGCCAGTGGCCGGACCCCTGAGGGACCTGACGAAGAAGATGTGTCTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207373 protein sequence
Red=Cloning site Green=Tags(s) MYSEYVKNFERAAELLATWMDKSQPFQEVVTRIQCSEASSSLTLQHHMLEPVQRIPRYELLLKEYVQKLP AQAPDLEDAQRALDMIFSAAQHSNAAIAEMERLQGLWDVYQRLGLEDDIVDPSNTLLREGPVLKISFRRS DPMERYLVLFNNMLLYCVPRVLQVGAQFQVRTRIDVAGMKVRELTDAEFPHSFLVSGKQRTLELQARSRD EMVSWMQACQAAIDQVEKRSETFKAAVQGPQGDTQEPKPQVEELGLRAPQWVRDKMVTMCMRCQEPFNAL TRRRHHCRACGYVVCAKCSDYRAELKYDSNRPNRVCLTCYTFLTGNVLPQGKEDKRRGILEKEASAAPEQ SLVCSFLQLIGDKCSRSLPRSWCVIPRDDPLVLYVYAAPQDTKAHTSIPLLGYQVISGPQGDPRVFQLQQ SGQQYTFKAESVELQGRWVTAIKRAASGRTPEGPDEEDVSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC021845 |
| ORF Size | 1383 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021845, AAH21845 |
| RefSeq Size | 2094 bp |
| RefSeq ORF | 1385 bp |
| Locus ID | 26382 |
| MW | 52.5 kDa |
| Gene Summary | Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217772 | Fgd2 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697), (10ug) |
CNY 6,940.00 |
|
| MG207373 | Fgd2 (tGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697) |
CNY 2,950.00 |
|
| MR207373L3 | Lenti ORF clone of Fgd2 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697) |
CNY 4,560.00 |
|
| MR207373L4 | Lenti ORF clone of Fgd2 (mGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 2 (cDNA clone MGC:30923 IMAGE:4016697) |
CNY 4,560.00 |
