Fktn (NM_139309) Mouse Tagged ORF Clone
CAT#: MR207355
- TrueORF®
Fktn (Myc-DDK-tagged) - Mouse fukutin (Fktn)
ORF Plasmid: tGFP
"NM_139309" in other vectors (3)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D830030O17Rik; Fcmd |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR207355 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGAATCAATAAGAACGTGGTCTTGGCCCTTTTAACATTGACGAGTTCTGCCTTTCTGCTGTTTC AGCTGTACTACTACAAGCACTATTTGTCTGCAAGGAATGGACTGGGTTCATCAAAATCCAAAGGGAATCG AGTTGGATTTGATAGCACACAGTGGCGTGCAGTTAAGAAATTTATTATGCTGACATCCAGCCAAAATGTG CCAGTGTTCCTTATTGACCCATGGATTCTGGAATCAATTAATAAAAACTTCGAACAAGTGAAAAATGCTT CTCAAGGCCCGGCTTCAGAATGCAGATTTTTCTGTGTTCCGAGAGACTTCACTGCGTTCGCACTGCAGTA TCACCTGTGGAAGAATGAGGATGGCTGGTTTCGGATAGCTGAGAATATGGGGTTTCAATGCCTAAAGACT GAAAGCAAGGATCCTCGGCTAGATGGAATAGACTCACTTTCTGGGACTGAAATCCCTCTGCACTACGTCT GTAAGCTGACCACTCATGCCATCCACTTGGTAGTCTTTCATGAGAGGAGTGGCAACTACCTCTGGCATGG TCACCTACGACTCAAAGGACACATGGATAGGAAGTTTGTTCCTTTTCGAAAGTTACAGTTTGGTCGTTAT CCTGGAGCCTTTGACAGGCCAGAGTTACAGCAAGTTACTGTTGATGGACTAGATATGCTTATTCCGAAAG ACCCAGGGCGCTTTCTAGAAGAAGTACCTCACTCCAGATTTATCGAGTGCAGGTATAAGGAAGCTCGGGC GTTCCTCCAGCAGTACATTGATGATAATACTGTGGATGCTATGGTCTTTCGAAAGAGGGCAAAGGAGTTA CTGCAACTAGCAGCCAAGACGCTGAAGGACCTGGGAGTGCCCTTCTGGCTGAGCAGTGGGACTTGTCTAG GATGGTATCGGCAGTGCGGTATTATTCCTTACAGCAAAGATGTCGACTTAGGGATTTTTATACAAGATTA CAAACCTGATATTATTTTGGCATTTCAGGAAGCAGGACTTCCACTCAAACACAAGTTTGGGAAGGTGGAA GACAGCTTGGAACTATCCTTCCAGGGGAAAAATGATGTAAAACTTGATATTTTTTTCTTCTATGAAGAGG CTGACCATCTGTGGAATGGAGGCACGCAGGCCAGAACGGGAAAAAAGTTTAAGTATCTGTTTCCCAAGTT TACACTGTGCTGGACTGAGTTTGTAGACATCAAAGTGCATGTTCCCTGTGAAACGGTTGACTACATTGAA GCCAACTATGGTAAGACCTGGAAGATTCCTATCAAGACATGGGACTGGAAGAGCTCACCCCCGAATGTGC AGCCCAATGGCATCTGGCCTATTTCGGAGTGGGATGAGGTTATCCAGTTGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207355 protein sequence
Red=Cloning site Green=Tags(s) MSRINKNVVLALLTLTSSAFLLFQLYYYKHYLSARNGLGSSKSKGNRVGFDSTQWRAVKKFIMLTSSQNV PVFLIDPWILESINKNFEQVKNASQGPASECRFFCVPRDFTAFALQYHLWKNEDGWFRIAENMGFQCLKT ESKDPRLDGIDSLSGTEIPLHYVCKLTTHAIHLVVFHERSGNYLWHGHLRLKGHMDRKFVPFRKLQFGRY PGAFDRPELQQVTVDGLDMLIPKDPGRFLEEVPHSRFIECRYKEARAFLQQYIDDNTVDAMVFRKRAKEL LQLAAKTLKDLGVPFWLSSGTCLGWYRQCGIIPYSKDVDLGIFIQDYKPDIILAFQEAGLPLKHKFGKVE DSLELSFQGKNDVKLDIFFFYEEADHLWNGGTQARTGKKFKYLFPKFTLCWTEFVDIKVHVPCETVDYIE ANYGKTWKIPIKTWDWKSSPPNVQPNGIWPISEWDEVIQLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_139309 |
ORF Size | 1386 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_139309.1, NM_139309.2, NM_139309.3, NM_139309.4, NP_647470.1 |
RefSeq Size | 3382 bp |
RefSeq ORF | 1386 bp |
Locus ID | 246179 |
UniProt ID | Q8R507 |
MW | 53.6 kDa |
Gene Summary | Catalyzes the transfer of CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1) (PubMed:12471058). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1 (By similarity). Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity (PubMed:19017726). May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes (PubMed:19017726, PubMed:22922256). Could be involved in brain development (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG207355 | Fktn (tGFP-tagged) - Mouse Fukuyama type congenital muscular dystrophy homolog (human) (Fcmd) |
CNY 3,710.00 |
|
MR207355L3 | Lenti ORF clone of Fktn (Myc-DDK-tagged) - Mouse fukutin (Fktn) |
CNY 5,230.00 |
|
MR207355L4 | Lenti ORF clone of Fktn (mGFP-tagged) - Mouse fukutin (Fktn) |
CNY 5,230.00 |