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Ppp2r2d (NM_026391) Mouse Tagged ORF Clone
CAT＃: MR207239
- TrueORF®
Ppp2r2d (Myc-DDK-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d)
ORF Plasmid: tGFP
"NM_026391" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300017E19Rik; D7Ertd753e; MDS026 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207239 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGAGCTGGAGGCGGCGGCTGCCCGGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTGA AGGGCGCTGTCGACGAGGACGTGGCGGAAGCCGACATCATCTCCACCGTTGAGTTTAACTACTCTGGAGA CCTTCTTGCAACAGGAGACAAAGGTGGCAGAGTTGTTATTTTCCAGCGGGAACAAGAGAATAAAGGCCGC GCTCACTCTAGGGGAGAGTACAATGTTTACAGTACCTTTCAGAGTCATGAGCCAGAGTTTGACTATTTGA AAAGTCTAGAAATTGAAGAAAAAATTAATAAAATCAGGTGGTTACCGCAACAGAATGCTGCTCATTTTCT ACTCTCTACAAATGATAAAACTATTAAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTAT AACTTGAAAGATGAAGATGGACGACTTCGAGACCCATTTAGAATTACGGCACTACGGGTTCCAATATTGA AGCCCATGGACCTTATGGTAGAAGCAAGTCCACGACGAATTTTTGCAAATGCTCATACATATCACATAAA TTCCATTTCAGTAAATAGTGATCATGAAACATATCTCTCTGCAGATGATCTGAGAATTAACCTATGGCAT TTAGAAATCACAGATAGAAGCTTCAACATTGTGGACATCAAGCCAGCTAATATGGAGGAGCTGACAGAAG TCATCACTGCCGCAGAGTTCCACCCACATCAGTGCAATGTATTTGTTTACAGCAGCAGCAAGGGCACCAT CAGGCTGTGTGACATGCGTTCCTCTGCCCTATGTGACAGGCATGCCAAGTTTTTTGAAGAGCCAGAAGAT CCCAGCAGTAGATCCTTCTTCTCAGAAATAATCTCATCTATATCTGATGTCAAGTTCAGCCACAGTGGTC GATACATGATGACCAGAGACTATCTGTCGGTGAAGGTCTGGGACCTCAACATGGAGGGCAGGCCTGTGGA GACCCACCAGGTACATGAGTACCTGCGAAGCAAGCTCTGCTCCTTGTATGAGAACGACTGCATCTTTGAC AAGTTCGAGTGCTGCTGGAACGGTTCAGACAGTGCCATTATGACGGGGTCCTACAACAACTTCTTTAGAA TGTTTGATAGAAACACTCGGAGGGATGTTACACTGGAAGCCTCAAGAGAGAACAGCAAACCCCGAGCCAG CCTGAAGCCCCGGAAAGTATGTACAGGGGGTAAGAGAAAGAAAGACGAGATTAGCGTGGACAGTTTGGAC TTCAATAAGAAGATCCTCCACACAGCCTGGCACCCCATGGAGAGCATTATTGCTGTAGCTGCCACCAATA ACTTGTATATATTCCAGGACAAAATTAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207239 protein sequence
Red=Cloning site Green=Tags(s) MAGAGGGGCPAGGNDFQWCFSQVKGAVDEDVAEADIISTVEFNYSGDLLATGDKGGRVVIFQREQENKGR AHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAHFLLSTNDKTIKLWKISERDKRAEGY NLKDEDGRLRDPFRITALRVPILKPMDLMVEASPRRIFANAHTYHINSISVNSDHETYLSADDLRINLWH LEITDRSFNIVDIKPANMEELTEVITAAEFHPHQCNVFVYSSSKGTIRLCDMRSSALCDRHAKFFEEPED PSSRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKVWDLNMEGRPVETHQVHEYLRSKLCSLYENDCIFD KFECCWNGSDSAIMTGSYNNFFRMFDRNTRRDVTLEASRENSKPRASLKPRKVCTGGKRKKDEISVDSLD FNKKILHTAWHPMESIIAVAATNNLYIFQDKIN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026391 |
| ORF Size | 1362 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026391.2, NP_080667.1 |
| RefSeq Size | 2079 bp |
| RefSeq ORF | 1362 bp |
| Locus ID | 52432 |
| UniProt ID | Q925E7 |
| MW | 52 kDa |
| Gene Summary | B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207239 | Ppp2r2d (tGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d) |
CNY 3,710.00 |
|
| MR207239L3 | Lenti ORF clone of Ppp2r2d (Myc-DDK-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d) |
CNY 5,230.00 |
|
| MR207239L4 | Lenti ORF clone of Ppp2r2d (mGFP-tagged) - Mouse protein phosphatase 2, regulatory subunit B, delta isoform (Ppp2r2d) |
CNY 5,230.00 |
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