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Rgma (BC023870) Mouse Tagged Lenti ORF Clone
CAT＃: MR206975L3
- LentiORF®
Lenti ORF clone of Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958)
"BC023870" in other vectors (4)
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CNY 4,370.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C230063O06, MGC38550, MGC69915 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR206975).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023870 |
| ORF Size | 1311 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023870, AAH23870 |
| RefSeq Size | 3384 bp |
| RefSeq ORF | 1313 bp |
| Locus ID | 244058 |
| Gene Summary | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218652 | Rgma (untagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958), (10ug) |
CNY 6,940.00 |
|
| MG206975 | Rgma (tGFP-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958) |
CNY 5,256.00 |
|
| MR206975 | Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958) |
CNY 3,656.00 |
|
| MR206975L4 | Lenti ORF clone of Rgma (mGFP-tagged) - Mouse RGM domain family, member A (cDNA clone MGC:38550 IMAGE:5353958) |
CNY 4,370.00 |
