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Mark3 (BC026445) Mouse Tagged ORF Clone
CAT＃: MR206826
- TrueORF®
Mark3 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (cDNA clone MGC:31426 IMAGE:4459439)
ORF Plasmid: tGFP
"BC026445" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ETK-1, Emk2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206826 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACAGGTGGATCAATGCAGGCCATGAGGAGGATGAGCTTAAGCCATTTGTTGAACCAGAACTAG ACATCTCAGACCAGAAGAGAATAGATATTATGGTGGGAATGGGATATTCCCAAGAAGAAATTCAAGAATC TCTTAGCAAAATGAAATACGATGAAATCACAGCTACTTACTTGCTACTGGGGAGGAAGTCTGCAGAGCTG GACGCTAGTGACTCCAGTTCTAGCAGCAACCTTTCACTTGCTAAGGTGCGGCCAAACAGTGACCTCAGCA ATAGCACTGGCCAGTCTCCTCATCACAAAGGGCAGAGAAGTGTTTCCTCAAGTCAGAAGCAGAGACGGTA CAGTGACCACGCTGGACCAGCTATTCCTTCAGTTGTGGCATATCCAAAGAGGAGTCAGACCAGCACTGCA GATAGTGACCTCAAAGAAGATGGAATTCCCTCCCGAAAATCAAGCAGCAGTGCTGTAGGAGGGAAGGGGA TTGCTCCTGCCAGTCCCATGCTTGGGAATGCAGGCAATCCCAATAAAGCCGATATCCCTGAGCGCAAGAA AAGCCCTGCTGTGCCCAGTAGTAACACAGCATCTGGTGGGATGACACGGCGGAACACTTACGTTTGCAGC GAGAGATGTGCAGCAGACAGACATTCTGTGATTCAGAACGGCAAAGAAAACAGCGCTATTCCAGATGAGA GAACTCCAGTTGCTTCAACCCACAGTATTAGCAGTGCGACCACCCCAGATCGCATCCGCTTTCCAAGGGG CACAGCCAGCCGTAGCACTTTCCATGGCCAACCTCGGGAGCGGCGAACCGCAACATATAATGGCCCACCT GCCTCACCCAGCTTGTCCCATGAAGCCACACCGCTGTCTCAGACTCGAAGCCGAGGTTCCACTAATCTTT TTAGTAAATTAACTTCCAAACTCACAAGAAGTCGCAATGTGTCTTCAGAGCAGAAGGATGAGAACAGAGA GGCCAAGCCCCGCTCCCTGCGCTTCACCTGGAGCATGAAGACCACGAGCTCCATGGACCCCAGTGACATG ATGCGGGAGATCCGCAAGGTGCTGGACGCCAATAACTGCGACTACGAGCAGAGGGAGCGCTTCCTGCTCT TCTGTGTCCATGGCGACGGGCACGCAGAGAACCTGGTACAGTGGGAAATGGAAGTCTGTAAGCTGCCAAG ACTGTCTCTGAATGGAGTCCGGTTTAAGCGGATATCAGGGACATCCATAGCTTTCAAAAATATTGCTTCC AAAATTGCCAATGAGCTAAAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206826 protein sequence
Red=Cloning site Green=Tags(s) MKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSAEL DASDSSSSSNLSLAKVRPNSDLSNSTGQSPHHKGQRSVSSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTA DSDLKEDGIPSRKSSSSAVGGKGIAPASPMLGNAGNPNKADIPERKKSPAVPSSNTASGGMTRRNTYVCS ERCAADRHSVIQNGKENSAIPDERTPVASTHSISSATTPDRIRFPRGTASRSTFHGQPRERRTATYNGPP ASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRSRNVSSEQKDENREAKPRSLRFTWSMKTTSSMDPSDM MREIRKVLDANNCDYEQRERFLLFCVHGDGHAENLVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIAS KIANELKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026445 |
| ORF Size | 1284 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026445, AAH26445 |
| RefSeq Size | 1865 bp |
| RefSeq ORF | 1286 bp |
| Locus ID | 17169 |
| MW | 47.2 kDa |
| Gene Summary | Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAPT/TAU, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217718 | Mark3 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (cDNA clone MGC:31426 IMAGE:4459439), (10ug) |
CNY 6,940.00 |
|
| MG206826 | Mark3 (tGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (cDNA clone MGC:31426 IMAGE:4459439) |
CNY 2,760.00 |
|
| MR206826L3 | Lenti ORF clone of Mark3 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (cDNA clone MGC:31426 IMAGE:4459439) |
CNY 4,370.00 |
|
| MR206826L4 | Lenti ORF clone of Mark3 (mGFP-tagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (cDNA clone MGC:31426 IMAGE:4459439) |
CNY 4,370.00 |
