United States
Germany
Japan
United Kingdom
China
Ubr7 (NM_025666) Mouse Tagged ORF Clone
CAT＃: MR206771
- TrueORF®
Ubr7 (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 7 (putative) (Ubr7)
ORF Plasmid: tGFP
"NM_025666" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730410I19Rik; AA589405; AW557761 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206771 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGCCGAGAGCCCCACCGAGTGCCAGGCGGAGCTGGAGCCCGTGGTGTCGCTGGTGGACGTGC TCGAGGAAGACGAGGAGTTGGAGAATGAGGCGTGCGCCGTCCTGGGCGGCAGCGACTCGGAGAAGTGCTC CTACTCGCAGGGCTCAGTGGGGAGACAAGCACTGTACGCCTGCAGCACCTGCACGCCAGAGGGAGAAGAG CCCGCAGGGATTTGTCTAGCTTGCAGTTACGAATGTCATGGAAGCCATAAACTGTTTGAACTATACACAA AAAGAAATTTTCGTTGTGATTGTGGAAACAGCAAGTTTAAAAACTTGGAATGCAAATTATTTCCTGACAA ATCAAAGGTAAATTCTTGCAATAAGTACAATGACAACTTTTTTGGATTATACTGCGTTTGTAAGAGACCT TATCCTGATCCTGAAGATGAGGTTCCAGATGAGATGATACAGTGTGTGGTCTGTGAAGACTGGTTCCATG GCAGGCATCTTGGTGCCATTCCCCCTGAGAGTGGGGATTTCCAGGAGATGGTGTGCCAGGCTTGTATGAG GCGCTGTTCTTTCTTGTGGGCTTATGCTGCCCAGTTGGCAGTCACCAGGATATCTGCTGAAGATGATGGG CTGCTGCCAAATGCTACTGGCATGGGAGATGAAGATGTTAGCAAGCCTGAAAATGGAGCTCCCCAAGATA ATGGCCTGAAAGAGGATGCTCCCGAGCATGGCAGGGATTCTGTCAACGAGGTGAAAGCTGAGCAGAAGAA CGAGCCCTGCAGCAGCTCTAGTTCTGAGTCTGACCTCCAGACAGTGTTTAAGAAAGAAAATATCAAAACT GAGCCACAGTCCAGCTGCAGACTTCAGGAACTCCAAGCCAAGCAGTTTGTAAAGAAAGATGCTGCCACCT ATTGGCCCTTGAACTGGCGCAGCAAACTATGCACCTGTCAAGACTGTATGAAAATGTATGGAGAACTAGA CGTCCTGTTCCTGACAGATGAATGTGATACAGTTCTGGCTTATGAGAACAAAGGCAAGAACGACCAGGCA ACTGATAGAAGGGACCCTTTAATGGACACCCTTAGCAGCATGAATAGAGTCCAGCAAGTGGAGCTCATCT GTGAATACAATGACTTGAAGACTGAACTTAAGGACTACCTGAAGAGATTTGCTGATGAAGGCACGGTTGT TAAGAGAGAAGACATTCAGCAGTTCTTTGAAGAGTTTCAATCAAAAAAGAGAAGGCGAGTGGACGGGTTA CAGTATTACTGCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206771 protein sequence
Red=Cloning site Green=Tags(s) MAGAESPTECQAELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVGRQALYACSTCTPEGEE PAGICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLFPDKSKVNSCNKYNDNFFGLYCVCKRP YPDPEDEVPDEMIQCVVCEDWFHGRHLGAIPPESGDFQEMVCQACMRRCSFLWAYAAQLAVTRISAEDDG LLPNATGMGDEDVSKPENGAPQDNGLKEDAPEHGRDSVNEVKAEQKNEPCSSSSSESDLQTVFKKENIKT EPQSSCRLQELQAKQFVKKDAATYWPLNWRSKLCTCQDCMKMYGELDVLFLTDECDTVLAYENKGKNDQA TDRRDPLMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRRRVDGL QYYCS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_025666 |
| ORF Size | 1278 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_025666.2, NP_079942.1 |
| RefSeq Size | 3247 bp |
| RefSeq ORF | 1278 bp |
| Locus ID | 66622 |
| UniProt ID | Q8BU04 |
| MW | 48.1 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207589 | Ubr7 (untagged) - Mouse ubiquitin protein ligase E3 component n-recognin 7 (putative) (Ubr7), (10ug) |
CNY 3,990.00 |
|
| MG206771 | Ubr7 (tGFP-tagged) - Mouse RIKEN cDNA 5730410I19 gene (5730410I19Rik) |
CNY 3,140.00 |
|
| MR206771L3 | Lenti ORF clone of Ubr7 (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 7 (putative) (Ubr7) |
CNY 4,750.00 |
|
| MR206771L4 | Lenti ORF clone of Ubr7 (mGFP-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 7 (putative) (Ubr7) |
CNY 4,750.00 |
