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Pacsin3 (NM_028733) Mouse Tagged ORF Clone
CAT＃: MR206762
- TrueORF®
Pacsin3 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3)
"NM_028733" in other vectors (2)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921507A02Rik; 6330413E15Rik; AW413130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206762 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCAGAGGAGGACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGGAGGCTGGCAACTACAGAC GGACGGTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGGACCTGGTTAGCTGCTTCCAAGAACGTGC CCGCATTGAGAAGGCCTACGCCCAGCAGCTGGCTGACTGGGCCCGCAAGTGGAGGGGTGCTGTGGAGAAG GGCCCACAGTACGGCACCCTGGAGAAGGCCTGGCATGCCTTCTTCACTGCGGCTGAGAGGCTGAGCGAGC TGCACTTGGAGGTGAGGGAGAAGTTGCACGGTCCAGACAGCGAGCGAGTGCGGACCTGGCAGCGAGGGGC TTTCCACCGGCCAGTGCTGGGGGGCTTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTCCGTAAAGCTCAG AAGCCCTGGCTAAAGAGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACCACACAGCCCGCAAGGATG AGAAGACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACAGCTCCATGTCCCAGGAGCAGCTTCGAAA ATTACAGGAGCGGGTGGGCCGCTGCACCAAGGAGGCAGAGAAGATGAAAACCCAGTATGAGCAGACCCTG GCCGAGCTAAATCGCTACACCCCACGCTACATGGAGGACATGGAGCAGGCTTTTGAGAGCTGCCAGGCTG CTGAGCGCCAGCGGCTTCTCTTCTTCAAGGATGTCTTGCTCACCCTGCATCAACACCTCGACCTCTCCAG TAGCGACAAGTTCCATGAACTCCATCGAGACCTGCAGCAGAGCATTGAGGCTGCCAGTGATGAAGAGGAT CTGCGCTGGTGGCGGAGCACACACGGGCCTGGCATGGCCATGAACTGGCCACAGTTTGAGGAATGGTCGT TAGACACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCCGGAGCCCAGATGAGGTTACTCTGACCAG CATTGTACCGACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCATCGTCCCCGGGTAGCGGGCAGGAC GAGGACTGGTCAGATGAGGAGAGTCCCCGGAAAGTTGCCACTGGGGTGAGGGTACGGGCGCTTTATGACT ATGCTGGCCAGGAGGCTGATGAGCTGAGCTTCCGAGCAGGGGAAGAGTTGCTGAAGATGAGCGAGGAGGA TGAGCAGGGCTGGTGCCAGGGCCAGCTGCAGAGTGGCCGCATTGGTCTGTACCCTGCCAATTACGTAGAA TGTGTGGGCGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206762 protein sequence
Red=Cloning site Green=Tags(s) MAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGAVEK GPQYGTLEKAWHAFFTAAERLSELHLEVREKLHGPDSERVRTWQRGAFHRPVLGGFRESRAAEDGFRKAQ KPWLKRLKEVEASKKSYHTARKDEKTAQTRESHAKADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTL AELNRYTPRYMEDMEQAFESCQAAERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAASDEED LRWWRSTHGPGMAMNWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPSSPGSGQD EDWSDEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLYPANYVE CVGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028733 |
| ORF Size | 1275 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_028733.4 |
| RefSeq Size | 1994 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 80708 |
| UniProt ID | Q99JB8 |
| MW | 48.6 kDa |
| Gene Summary | Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR206762L3 | Lenti ORF clone of Pacsin3 (Myc-DDK-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3) |
CNY 4,750.00 |
|
| MR206762L4 | Lenti ORF clone of Pacsin3 (mGFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3) |
CNY 4,750.00 |
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