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Dnmt3l (NM_019448) Mouse Tagged ORF Clone
CAT＃: MR206697
- TrueORF®
Dnmt3l (Myc-DDK-tagged) - Mouse DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l), transcript variant 1
ORF Plasmid: tGFP
"NM_019448" in other vectors (3)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D6Ertd14; D6Ertd14e; ecat; ecat7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206697 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTCCCGGGAGACACCTTCTTCTTGCTCTAAGACCCTTGAAACCTTGGACCTGGAGACTTCCGACA GCTCTAGCCCTGATGCTGACAGTCCTCTGGAAGAGCAATGGCTGAAATCCTCCCCAGCCCTGAAGGAGGA CAGTGTGGATGTGGTACTGGAAGACTGCAAAGAGCCTCTGTCCCCCTCCTCGCCTCCGACAGGCAGAGAG ATGATCAGGTACGAAGTCAAAGTGAACCGACGGAGCATTGAAGACATCTGCCTCTGCTGTGGAACTCTCC AGGTGTACACTCGGCACCCCTTGTTTGAGGGAGGGTTATGTGCCCCATGTAAGGATAAGTTCCTGGAGTC CCTCTTCCTGTATGATGATGATGGACACCAGAGTTACTGCACCATCTGCTGTTCCGGGGGTACCCTGTTC ATCTGTGAGAGCCCCGACTGTACCAGATGCTACTGTTTCGAGTGTGTGGACATCCTGGTGGGCCCCGGGA CCTCAGAGAGGATCAATGCCATGGCCTGCTGGGTTTGCTTCCTGTGCCTGCCCTTCTCACGGAGTGGACT GCTGCAGAGGCGCAAGAGGTGGCGGCACCAGCTGAAGGCCTTCCATGATCAAGAGGGAGCGGGCCCTATG GAGATATACAAGACAGTGTCTGCATGGAAGAGACAGCCAGTGCGGGTACTGAGCCTTTTTAGAAATATTG ATAAAGTACTAAAGAGTTTGGGCTTTTTGGAAAGCGGTTCTGGTTCTGGGGGAGGAACGCTGAAGTACGT GGAAGATGTCACAAATGTCGTGAGGAGAGACGTGGAGAAATGGGGCCCCTTTGACCTGGTGTACGGCTCG ACGCAGCCCCTAGGCAGCTCTTGTGATCGCTGTCCCGGCTGGTACATGTTCCAGTTCCACCGGATCCTGC AGTATGCGCTGCCTCGCCAGGAGAGTCAGCGGCCCTTCTTCTGGATATTCATGGACAATCTGCTGCTGAC TGAGGATGACCAAGAGACAACTACCCGCTTCCTTCAGACAGAGGCTGTGACCCTCCAGGATGTCCGTGGC AGAGACTACCAGAATGCTATGCGGGTGTGGAGCAACATTCCAGGGCTGAAGAGCAAGCATGCGCCCCTGA CCCCAAAGGAAGAAGAGTATCTGCAAGCCCAAGTCAGAAGCAGGAGCAAGCTGGACGCCCCGAAAGTTGA CCTCCTGGTGAAGAACTGCCTTCTCCCGCTGAGAGAGTACTTCAAGTATTTTTCCCAAAACTCACTTCCT CTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206697 protein sequence
Red=Cloning site Green=Tags(s) MGSRETPSSCSKTLETLDLETSDSSSPDADSPLEEQWLKSSPALKEDSVDVVLEDCKEPLSPSSPPTGRE MIRYEVKVNRRSIEDICLCCGTLQVYTRHPLFEGGLCAPCKDKFLESLFLYDDDGHQSYCTICCSGGTLF ICESPDCTRCYCFECVDILVGPGTSERINAMACWVCFLCLPFSRSGLLQRRKRWRHQLKAFHDQEGAGPM EIYKTVSAWKRQPVRVLSLFRNIDKVLKSLGFLESGSGSGGGTLKYVEDVTNVVRRDVEKWGPFDLVYGS TQPLGSSCDRCPGWYMFQFHRILQYALPRQESQRPFFWIFMDNLLLTEDDQETTTRFLQTEAVTLQDVRG RDYQNAMRVWSNIPGLKSKHAPLTPKEEEYLQAQVRSRSKLDAPKVDLLVKNCLLPLREYFKYFSQNSLP L TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019448 |
| ORF Size | 1266 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019448.1, NM_019448.2, NM_019448.3, NM_019448.4, NP_062321.1 |
| RefSeq Size | 1685 bp |
| RefSeq ORF | 1266 bp |
| Locus ID | 54427 |
| UniProt ID | Q9CWR8 |
| MW | 48 kDa |
| Gene Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein that is a catalytically inactive regulatory factor of DNA methyltransferases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206697 | Dnmt3l (tGFP-tagged) - Mouse DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l), transcript variant 1 |
CNY 3,140.00 |
|
| MR206697L3 | Lenti ORF clone of Dnmt3l (Myc-DDK-tagged) - Mouse DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l), transcript variant 1 |
CNY 4,750.00 |
|
| MR206697L4 | Lenti ORF clone of Dnmt3l (mGFP-tagged) - Mouse DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l), transcript variant 1 |
CNY 4,750.00 |
