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Itpk1 (NM_172584) Mouse Tagged ORF Clone
CAT＃: MR206652
- TrueORF®
Itpk1 (Myc-DDK-tagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1)
ORF Plasmid: tGFP
"NM_172584" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC031182 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206652 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACCTTCCTGAAAGGGAAGAGAGTTGGCTACTGGCTGAGCGAGAAGAAAGTCAAGAAGCTCAATT TCCAGGCCTTCGCGGAGCTGTGCAGGAAGCGAGGGATAGAAGTCGTGCAGCTGAACCTCAGCAGACCGAT TGAGGAGCAGGGCCCCCTGGACGTCATCATCCACAAGCTGACCGATGTCATCCTCGAGGCAGACCAGAAT GACAGCCAGTCCCTGGAATTGGTGCACAGGTTCCAAGAGTACATCGATGCTCACCCTGAGACCATCGTCT TGGATCCCCTCCCCGCCATCAGGACCCTGTTAGACCGTTCCAAGTCCTACGAACTTATCCGAAAGATCGA GGCCTACATGAAAGATGACAGAATCTGCTCGCCGCCTTTCATGGAGCTCACGAGCCTGTGTGGGGAGGAC ACCATGAGGCTGCTGGAGCAGAACGGCCTGGCCTTCCCCTTCATTTGCAAAACCAGAGTGGCTCATGGAA CCAACTCTCATGAGATGGCTATTGTGTTCAACCAAGAGGGCCTGAATGCCATCCAGCCTCCCTGTGTGGT CCAGAACTTCATCAACCACAATGCTGTCCTGTACAAGGTGTTTGTGGTGGGCGAGTCCTACACAGTGGTC CAGAGACCCTCACTCAAGAACTTCTCTGCGGGCACATCAGATCGTGAGTCCATCTTCTTCAACAGCCACA ATGTGTCAAAGCCGGAGTCTTCATCAGTCCTCACTGAGCTGGACAAGATCGAGGGTGTGTTCGAACGGCC AAGCGATGAGGTTATCCGGGAGCTGTCCCGGGCTCTGCGGCAGGCGCTGGGAGTGTCACTGTTTGGAATT GACATCATCATTAACAACCAGACCGGGCAGCATGCAGTCATCGATGTCAATGCCTTCCCAGGCTATGAAG GAGTGAGTGAGTTCTTTACCGACCTCCTGAACCACATTGCCACAGTTCTGCAAGGCCAGAGCACGGGAGG AGCTGCCACGGAGGAAGTGGCCCCGCTAAGGCACAACAGGCTCCTGGCGGAACCGGCAGGCAGCCTGGCT GGGGAGCGGACGTGCAGTGCCAGCCCTGGCTGCTGTGGCAGCATGAAGGGCCAGGACACACCCTGGAAGA CTGAGACCGAAGCAGGCAACATGGGCGCTGGTGCTTCCGCCAAGCTGCCGCACCAGAGACTTGGCTGCAC CACTGGCGTATCACCCAGCTTCCAGCAGCACTGTGTGGCCTCTCTGGCCACCAAGGCTTCCTCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206652 protein sequence
Red=Cloning site Green=Tags(s) MQTFLKGKRVGYWLSEKKVKKLNFQAFAELCRKRGIEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQN DSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMKDDRICSPPFMELTSLCGED TMRLLEQNGLAFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVV QRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGI DIIINNQTGQHAVIDVNAFPGYEGVSEFFTDLLNHIATVLQGQSTGGAATEEVAPLRHNRLLAEPAGSLA GERTCSASPGCCGSMKGQDTPWKTETEAGNMGAGASAKLPHQRLGCTTGVSPSFQQHCVASLATKASSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172584 |
| ORF Size | 1260 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172584.3 |
| RefSeq Size | 2856 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 217837 |
| UniProt ID | Q8BYN3 |
| MW | 46.1 kDa |
| Gene Summary | Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain (By similarity). Plays an important role in MLKL-mediated necroptosis. Produces highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which bind to MLKL mediating the release of an N-terminal auto-inhibitory region leading to its activation. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205100 | Itpk1 (untagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1), (10ug) |
CNY 2,000.00 |
|
| MG206652 | Itpk1 (tGFP-tagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1) |
CNY 3,710.00 |
|
| MR206652L3 | Lenti ORF clone of Itpk1 (Myc-DDK-tagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1) |
CNY 5,230.00 |
|
| MR206652L4 | Lenti ORF clone of Itpk1 (mGFP-tagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1) |
CNY 5,230.00 |
