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Acss1 (BC030930) Mouse Tagged ORF Clone
CAT＃: MR206638
- TrueORF®
Acss1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679)
ORF Plasmid: tGFP
"BC030930" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110032O15Rik; Acas2; Acas2l; AceCS2; AI788978 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206638 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGGAGGCCCCTGTTTGCACCCCTGAGAGCATGAGCAGTGAAGACATGCTCTTTATGCTCTACA CCTCAGGGAGCACCGGGACACCCAAGGGACTCGTTCATACACAGGCAGGCTATCTACTGTATGCCGCCAT GACGCACAAGCTCGTGTTTGACTACCAGCCAGGTGATGTCTTTGGCTGTGTGGCTGACATCGGTTGTATC ACAGGACACAGCTATGTGGTGTATGGACCCCTCTGCAATGGAGCTACCACAGTCCTTTTTGAGAGCACCC CAGTTTACCCTGATGCTGGTCGTTACTGGGAGACAGTGCAGAGGCTAAAGATCAACCAGTTCTATGGTGC CCCGACAGCTGTCCGGCTGCTGCTGAAGTATGGGGATGCCTGGGTGAAAAAGTATGACCGCTCTTCCCTG CGCACACTGGGGTCAGTGGGAGAGCCTATCAACCACGAAGCCTGGGAGTGGCTCCACAAAGTCGTGGGTG ATGGCAGATGTACACTGGTGGACACTTGGTGGCAAACGGAAACTGGAGGCATCTGCATTGCACCACGGCC CTCGGAAGATGGGGCAGAGATCCTCCCGGGCATGGCCATGAGGCCGTTTTTTGGCATCGTTCCTGTACTC ATGGATGAGAAGGGCAATGTTTTGGAGGGTGGAGATGTCTCTGGGGCCTTGTGTATTTCCCAAGCTTGGC CAGGCATGGCAAGGACCATCTATGGTGACCACCAGAGGTTCGTAGATGCCTACTTCAGAGCGTACCCAGG TTATTACTTCACTGGAGACGGAGCTCACCGGACAGAGGGTGGCTATTACCAGATCACGGGGCGCATGGAT GATGTCATCAATATCAGTGGTCATCGCCTGGGGACTGCAGAGATTGAGGATGCAATGGCTGACCATCCCG CTGTTCCAGAGACTGCTGTCATTGGGTACCCTCATGATATCAAAGGAGAAGCTGCATTTGCCTTCATTGT GCTGAAAGATAACATCAGTGATGAGAACATGGTAGTGAATGAACTCAAATTGTCGGTGGCCACCAAGATC GCCAAGTATGCTGTGCCTGACCAGATCCTGGTGGTGAAGCGTCTCCCCAAAACCAGATCTGGGAAAGTGA TGAGGAGACTACTGAGGAAGATCATCACCAGCAGGGGACAGGATCTAGGGGACACCACTACCCTGGAGGA CCCCAGCGTCATCACAGAAATCTTGAGTGCCTTCCAGAAGTATGAAGAGCAGCGGGCTGCTACCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206638 protein sequence
Red=Cloning site Green=Tags(s) MAKEAPVCTPESMSSEDMLFMLYTSGSTGTPKGLVHTQAGYLLYAAMTHKLVFDYQPGDVFGCVADIGCI TGHSYVVYGPLCNGATTVLFESTPVYPDAGRYWETVQRLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSL RTLGSVGEPINHEAWEWLHKVVGDGRCTLVDTWWQTETGGICIAPRPSEDGAEILPGMAMRPFFGIVPVL MDEKGNVLEGGDVSGALCISQAWPGMARTIYGDHQRFVDAYFRAYPGYYFTGDGAHRTEGGYYQITGRMD DVINISGHRLGTAEIEDAMADHPAVPETAVIGYPHDIKGEAAFAFIVLKDNISDENMVVNELKLSVATKI AKYAVPDQILVVKRLPKTRSGKVMRRLLRKIITSRGQDLGDTTTLEDPSVITEILSAFQKYEEQRAATN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC030930 |
| ORF Size | 1257 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC030930, AAH30930 |
| RefSeq Size | 2783 bp |
| RefSeq ORF | 1259 bp |
| Locus ID | 68738 |
| MW | 46.2 kDa |
| Gene Summary | Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217858 | Acss1 (untagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679), (10ug) |
CNY 6,940.00 |
|
| MG206638 | Acss1 (tGFP-tagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679) |
CNY 2,660.00 |
|
| MR206638L3 | Lenti ORF clone of Acss1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679) |
CNY 4,280.00 |
|
| MR206638L4 | Lenti ORF clone of Acss1 (mGFP-tagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679) |
CNY 4,280.00 |
