United States
Germany
Japan
United Kingdom
China
Bmp8a (BC052168) Mouse Tagged ORF Clone
CAT＃: MR206487
- TrueORF®
Bmp8a (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507)
ORF Plasmid: tGFP
"BC052168" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OP2, OP-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206487 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGCGTCCCGGGCCACTCTGGCTATTGGGCCTTGCTCTGTGCGCGCTGGGAGGCGGCCACGGTC CGCGTCCCCCGCACACCTGTCCCCAGCGTCGCCTGGGAGCGCGCGAGCGCCGCGACATGCAGCGTGAAAT CCTGGCGGTGCTCGGGCTACCGGGACGGCCCCGACCCCGTGCACAACCCGCCGCTGCCCGGCAGCCAGCG TCCGCGCCCCTCTTCATGTTGGACCTATACCACGCCATGACCGATGACGACGACGGCGGGCCACCACAGG CTCACTTAGGCCGTGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAACGCGACCGTACCCTGGGCTA CCAGGAGCCACACTGGAAGGAATTCCACTTTGACCTAACCCAGATCCCTGCTGGGGAGGCTGTCACAGCT GCTGAGTTCCGGATCTACAAAGAACCCAGCACCCACCCGCTCAACACAACCCTCCACATCAGCATGTTCG AAGTGGTCCAAGAGCACTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCTCCGATCTGG GGACGAGGGCTGGCTGGTGCTGGACATCACAGCAGCCAGTGACCGATGGCTGCTGAACCATCACAAGGAC CTGGGACTCCGCCTCTATGTGGAAACCGCGGATGGGCACAGCATGGATCCTGGCCTGGCTGGTCTGCTTG GACGACAAGCACCACGCTCCAGACAGCCTTTCATGGTAACCTTCTTCAGGGCCAGCCAGAGTCCTGTGCG GGCCCCTCGGGCAGCGAGACCACTGAAGAGGAGGCAGCCAAAGAAAACGAACGAGCTTCCGCACCCCAAC AAACTCCCAGGGATCTTTGATGATGGCCACGGTTCCCGCGGCAGAGAGGTTTGCCGCAGGCATGAGCTCT ACGTCAGCTTCCGTGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAGGGCTACTCTGCCTATTACTG TGAGGGGGAGTGTGCTTTCCCACTGGACTCCTGTATGAACGCCACCAACCATGCCATCTTGCAGTCTCTG GTCAGTACCACAGTAGCCTGCTGCGACAGGTGGAGCGGGGTGCACCTGATGAAGCCAGATGTTGTCCCCA AGGCATGCTGTGCACCCACCAAACTGAGTGCCACCTCTGTGCTGTACTATGACAGCAGCAACAATGTCAT CCTGCGTAAACACCGTAACATGGTGGTCAAGGCCTGTGGCTGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206487 protein sequence
Red=Cloning site Green=Tags(s) MAMRPGPLWLLGLALCALGGGHGPRPPHTCPQRRLGARERRDMQREILAVLGLPGRPRPRAQPAAARQPA SAPLFMLDLYHAMTDDDDGGPPQAHLGRADLVMSFVNMVERDRTLGYQEPHWKEFHFDLTQIPAGEAVTA AEFRIYKEPSTHPLNTTLHISMFEVVQEHSNRESDLFFLDLQTLRSGDEGWLVLDITAASDRWLLNHHKD LGLRLYVETADGHSMDPGLAGLLGRQAPRSRQPFMVTFFRASQSPVRAPRAARPLKRRQPKKTNELPHPN KLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSL VSTTVACCDRWSGVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052168 |
| ORF Size | 1236 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052168, AAH52168 |
| RefSeq Size | 2412 bp |
| RefSeq ORF | 1238 bp |
| Locus ID | 12163 |
| MW | 46.1 kDa |
| Gene Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. Mice lacking a functional copy of this gene exhibit degeneration of germ cells and the epididymal epithelium. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218141 | Bmp8a (untagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507), (10ug) |
CNY 6,940.00 |
|
| MG206487 | Bmp8a (tGFP-tagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507) |
CNY 2,660.00 |
|
| MR206487L3 | Lenti ORF clone of Bmp8a (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507) |
CNY 4,280.00 |
|
| MR206487L4 | Lenti ORF clone of Bmp8a (mGFP-tagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507) |
CNY 4,280.00 |
