Bmp8a (BC052168) Mouse Tagged ORF Clone

CAT#: MR206487

  • TrueORF®

Bmp8a (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8a (cDNA clone MGC:59383 IMAGE:6334507)

ORF Plasmid: DDK tGFP



  "BC052168" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms OP2, OP-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206487 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTATGCGTCCCGGGCCACTCTGGCTATTGGGCCTTGCTCTGTGCGCGCTGGGAGGCGGCCACGGTC
CGCGTCCCCCGCACACCTGTCCCCAGCGTCGCCTGGGAGCGCGCGAGCGCCGCGACATGCAGCGTGAAAT
CCTGGCGGTGCTCGGGCTACCGGGACGGCCCCGACCCCGTGCACAACCCGCCGCTGCCCGGCAGCCAGCG
TCCGCGCCCCTCTTCATGTTGGACCTATACCACGCCATGACCGATGACGACGACGGCGGGCCACCACAGG
CTCACTTAGGCCGTGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAACGCGACCGTACCCTGGGCTA
CCAGGAGCCACACTGGAAGGAATTCCACTTTGACCTAACCCAGATCCCTGCTGGGGAGGCTGTCACAGCT
GCTGAGTTCCGGATCTACAAAGAACCCAGCACCCACCCGCTCAACACAACCCTCCACATCAGCATGTTCG
AAGTGGTCCAAGAGCACTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCTCCGATCTGG
GGACGAGGGCTGGCTGGTGCTGGACATCACAGCAGCCAGTGACCGATGGCTGCTGAACCATCACAAGGAC
CTGGGACTCCGCCTCTATGTGGAAACCGCGGATGGGCACAGCATGGATCCTGGCCTGGCTGGTCTGCTTG
GACGACAAGCACCACGCTCCAGACAGCCTTTCATGGTAACCTTCTTCAGGGCCAGCCAGAGTCCTGTGCG
GGCCCCTCGGGCAGCGAGACCACTGAAGAGGAGGCAGCCAAAGAAAACGAACGAGCTTCCGCACCCCAAC
AAACTCCCAGGGATCTTTGATGATGGCCACGGTTCCCGCGGCAGAGAGGTTTGCCGCAGGCATGAGCTCT
ACGTCAGCTTCCGTGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAGGGCTACTCTGCCTATTACTG
TGAGGGGGAGTGTGCTTTCCCACTGGACTCCTGTATGAACGCCACCAACCATGCCATCTTGCAGTCTCTG
GTCAGTACCACAGTAGCCTGCTGCGACAGGTGGAGCGGGGTGCACCTGATGAAGCCAGATGTTGTCCCCA
AGGCATGCTGTGCACCCACCAAACTGAGTGCCACCTCTGTGCTGTACTATGACAGCAGCAACAATGTCAT
CCTGCGTAAACACCGTAACATGGTGGTCAAGGCCTGTGGCTGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206487 protein sequence
Red=Cloning site Green=Tags(s)

MAMRPGPLWLLGLALCALGGGHGPRPPHTCPQRRLGARERRDMQREILAVLGLPGRPRPRAQPAAARQPA
SAPLFMLDLYHAMTDDDDGGPPQAHLGRADLVMSFVNMVERDRTLGYQEPHWKEFHFDLTQIPAGEAVTA
AEFRIYKEPSTHPLNTTLHISMFEVVQEHSNRESDLFFLDLQTLRSGDEGWLVLDITAASDRWLLNHHKD
LGLRLYVETADGHSMDPGLAGLLGRQAPRSRQPFMVTFFRASQSPVRAPRAARPLKRRQPKKTNELPHPN
KLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSL
VSTTVACCDRWSGVHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052168
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052168, AAH52168
RefSeq Size 2412 bp
RefSeq ORF 1238 bp
Locus ID 12163
MW 46.1 kDa
Gene Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. Mice lacking a functional copy of this gene exhibit degeneration of germ cells and the epididymal epithelium. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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