Mak (BC050009) Mouse Tagged ORF Clone

CAT#: MR206262

  • TrueORF®

Mak (Myc-DDK-tagged) - Mouse male germ cell-associated kinase (cDNA clone MGC:25408 IMAGE:4527843)

ORF Plasmid: DDK tGFP



  "BC050009" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms A930010O05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206262 representing BC050009
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGATACACAACCATGAAGCAGCTGGGAGACGGCACGTATGGGAGTGTACTGATGGGCAAGAGCA
ATGAGTCTGGGGAGCTGGTGGCAATCAAGAGGATGAAGAGAAAGTTCTATTCTTGGGATGAGTGTATGAA
CTTGCGAGAAGTTAAGTCCCTGAAAAAACTCAATCACGCCAATGTGATTAAACTAAAAGAAGTTATCAGA
GAGAATGACCATCTTTATTTTGTATTTGAATATATGAAAGAAAACCTCTATCAGCTAATGAAAGACAGAA
ACAAACTGTTCCCTGAGTCAGTCATCAGAAATATTATGTATCAGATACTACAGGGGCTGGCATTTATCCA
TAAACACGGCTTTTTTCACAGGGACATGAAACCTGAGAACTTGCTCTGCATGGGCCCAGAGCTGGTGAAG
ATTGCTGATTTTGGACTTGCAAGAGAATTAAGATCACAGCCACCATATACTGACTATGTGTCTACCAGAT
GGTACCGGGCTCCTGAAGTCTTACTAAGGTCTTCGGTGTACAGCTCTCCCATTGACGTGTGGGCCGTGGG
AAGTATAATGGCCGAGTTATATACATTTAGGCCGCTTTTCCCAGGGACAAGTGAAGTTGATGAGATCTTT
AAAATCTGCCAAGTGTTAGGGACTCCCAAGAAGAGTGACTGGCCGGAAGGATACCAGCTTGCATCCTCCA
TGAACTTCCGCTTTCCCCAGTGCATTCCCATCAACCTAAAAACTCTCATTCCCAATGCCAGTAGCGAGGC
CATCCAGCTCATGACAGAGATGCTGAACTGGGATCCAAAGAAGCGACCAACAGCAAGCCAGGCACTGAAA
CACCCATATTTCCAAGTCGGTCAGGTACTGGGCTCTTCTGCACACCATCTGGATACAAAACAGACTTTAC
ACAAGCAGCTGCAGCCTCTGGAGCCAAAGCCATCTTCCTCTGAACGGGATCCTAAGCCTTTGCCAAACAT
ACTTGATCAGCCTGCCGGGCAGCCCCAGCCAAAACAGGGCCACCAACCACTGCAGACCATTCAGCCACCA
CAGAACACAGTGACTCACCCACCCCCAAAGCAGCAGGGTCACCAGAAACCACCACAAACCATGTTTCCAA
GTATCATCAAAACCATCCCAGTGGTGAGTAGCCAAGCCGATGCTTTAGATGACACGGACTGGTGCACTCT
GGGTAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206262 representing BC050009
Red=Cloning site Green=Tags(s)

MNRYTTMKQLGDGTYGSVLMGKSNESGELVAIKRMKRKFYSWDECMNLREVKSLKKLNHANVIKLKEVIR
ENDHLYFVFEYMKENLYQLMKDRNKLFPESVIRNIMYQILQGLAFIHKHGFFHRDMKPENLLCMGPELVK
IADFGLARELRSQPPYTDYVSTRWYRAPEVLLRSSVYSSPIDVWAVGSIMAELYTFRPLFPGTSEVDEIF
KICQVLGTPKKSDWPEGYQLASSMNFRFPQCIPINLKTLIPNASSEAIQLMTEMLNWDPKKRPTASQALK
HPYFQVGQVLGSSAHHLDTKQTLHKQLQPLEPKPSSSERDPKPLPNILDQPAGQPQPKQGHQPLQTIQPP
QNTVTHPPPKQQGHQKPPQTMFPSIIKTIPVVSSQADALDDTDWCTLGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC050009
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC050009
RefSeq Size 6818 bp
RefSeq ORF 1199 bp
Locus ID 17152
MW 249.9 kDa
Gene Summary Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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