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Kiss1r (NM_053244) Mouse Tagged ORF Clone
CAT＃: MR206203
- TrueORF®
Kiss1r (Myc-DDK-tagged) - Mouse KISS1 receptor (Kiss1r)
ORF Plasmid: tGFP
"NM_053244" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gpr54; KiSS-1; kiSS-1R |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206203 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCGAGGCGACATTGGCTCCCAATGTGACCTGGTGGGCTCCGTCCAACGCTTCAGGATGCCCAG GCTGCGGTGTCAACGCCTCGGATGACCCAGGCTCTGCGCCAAGGCCCCTGGATGCCTGGCTGGTTCCCCT GTTTTTCGCTACACTCATGTTGCTTGGGCTGGTCGGAAACTCATTGGTCATCTACGTTATCTGCCGCCAC AAGCACATGCAGACAGTTACCAACTTCTACATCGCTAACCTGGCTGCCACAGACGTCACTTTCCTACTGT GCTGCGTGCCCTTCACCGCACTCCTCTACCCGCTGCCCGCCTGGGTGCTGGGAGACTTCATGTGCAAATT CGTCAACTACATCCAGCAGGTCTCGGTGCAAGCCACATGTGCCACTCTGACGGCCATGAGTGTGGACCGC TGGTATGTGACTGTGTTCCCGCTGCGTGCACTTCACCGCCGCACTCCGCGCCTGGCCCTGGCTGTCAGCC TCAGCATCTGGGTGGGGTCAGCAGCTGTGTCCGCCCCGGTGCTGGCCCTGCACCGCCTGTCGCCAGGGCC TCGCACCTACTGCAGCGAGGCGTTTCCCAGCCGCGCCCTGGAGCGCGCCTTCGCGCTCTACAACCTGCTG GCTCTATATCTGCTGCCGCTGCTCGCCACCTGCGCCTGCTACGGCGCCATGCTGCGCCACCTGGGCCGTG CGGCTGTACGCCCCGCACCCACTGACGGCGCCCTGCAGGGACAGCTGCTAGCACAGCGCGCCGGAGCAGT GCGCACCAAGGTCTCCCGGCTGGTGGCCGCTGTCGTCCTGCTCTTCGCCGCCTGCTGGGGCCCGATCCAG CTGTTCCTGGTGCTTCAAGCCCTGGGCCCCTCGGGGGCCTGGCACCCTCGAAGCTATGCCGCCTACGCGG TCAAGATCTGGGCTCACTGCATGTCCTACAGCAACTCGGCGCTCAATCCGCTGCTCTATGCCTTCCTGGG TTCACACTTCAGACAGGCCTTCTGCCGCGTGTGCCCCTGCTGCCGGCAACGCCAGCGCCGGCCCCACACG TCAGCGCACTCGGACCGAGCTGCAACTCACACTGTGCCGCACAGCCGTGCTGCGCACCCTGTGCGGATCA GGAGCCCGGAGCCTGGGAACCCTGTGGTGCGCTCGCCCTGCGCTCAGAGTGAACGCACTGCCTCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206203 protein sequence
Red=Cloning site Green=Tags(s) MATEATLAPNVTWWAPSNASGCPGCGVNASDDPGSAPRPLDAWLVPLFFATLMLLGLVGNSLVIYVICRH KHMQTVTNFYIANLAATDVTFLLCCVPFTALLYPLPAWVLGDFMCKFVNYIQQVSVQATCATLTAMSVDR WYVTVFPLRALHRRTPRLALAVSLSIWVGSAAVSAPVLALHRLSPGPRTYCSEAFPSRALERAFALYNLL ALYLLPLLATCACYGAMLRHLGRAAVRPAPTDGALQGQLLAQRAGAVRTKVSRLVAAVVLLFAACWGPIQ LFLVLQALGPSGAWHPRSYAAYAVKIWAHCMSYSNSALNPLLYAFLGSHFRQAFCRVCPCCRQRQRRPHT SAHSDRAATHTVPHSRAAHPVRIRSPEPGNPVVRSPCAQSERTASL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053244 |
| ORF Size | 1191 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053244.5 |
| RefSeq Size | 3163 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 114229 |
| UniProt ID | Q91V45 |
| MW | 43.1 kDa |
| Gene Summary | Receptor for metastin (kisspeptin-52 or kp-52), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205761 | Kiss1r (untagged) - Mouse KISS1 receptor (Kiss1r), (10ug) |
CNY 5,488.00 |
|
| MG206203 | Kiss1r (tGFP-tagged) - Mouse KISS1 receptor (Kiss1r) |
CNY 4,370.00 |
|
| MR206203L1 | Lenti ORF clone of Kiss1r (Myc-DDK-tagged) - Mouse KISS1 receptor (Kiss1r) |
CNY 7,888.00 |
|
| MR206203L2 | Lenti ORF clone of Kiss1r (mGFP-tagged) - Mouse KISS1 receptor (Kiss1r) |
CNY 5,890.00 |
|
| MR206203L3 | Lenti ORF clone of Kiss1r (Myc-DDK-tagged) - Mouse KISS1 receptor (Kiss1r) |
CNY 5,890.00 |
|
| MR206203L4 | Lenti ORF clone of Kiss1r (mGFP-tagged) - Mouse KISS1 receptor (Kiss1r) |
CNY 7,888.00 |
