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Gnb5 (NM_010313) Mouse Tagged ORF Clone
CAT＃: MR206192
- TrueORF®
Gnb5 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1
ORF Plasmid: tGFP
"NM_010313" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | flr; Gbeta5; GBS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206192 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCGATCAGACCTTCCTGGTTAATGTGTTCGGCTCATGTGACAAATGCTTCAAACAAAGGGCCCTAA GACCCGTTTTCAAGAAATCTCAACAACTCAACTACTGCTCCACGTGTGCAGAGATCATGGCAACCGATGG GCTGCACGAGAACGAGACGCTGGCGTCGCTGAAGAGCGAGGCCGAGAGCCTCAAGGGCAAGCTAGAGGAG GAGCGGGCAAAGCTGCATGACGTGGAGCTACACCAGGTGGCCGAGCGGGTGGAGGCCCTGGGCCAGTTTG TCATGAAGACCAGAAGGACCCTCAAAGGCCACGGGAACAAAGTCCTGTGCATGGATTGGTGCAAAGACAA GCGGAGAATCGTGAGCTCCTCACAGGATGGGAAGGTGATTGTCTGGGATTCCTTCACTACGAACAAGGAG CACGCGGTCACCATGCCTTGCACCTGGGTGATGGCGTGTGCTTACGCGCCATCGGGATGCGCCATTGCTT GTGGTGGTCTGGATAATAAGTGCTCTGTGTATCCACTGACATTCGACAAGAACGAGAACATGGCCGCCAA GAAGAAGTCTGTTGCTATGCACACCAACTACCTGTCTGCCTGCAGCTTCACCAACTCTGACATGCAGATT CTGACAGCCAGCGGGGATGGCACCTGCGCCCTGTGGGATGTGGAAAGTGGACAGCTGTTACAGAGCTTCC ATGGGCACGGGGCTGACGTCCTCTGCTTGGACCTGGCCCCCTCGGAAACAGGAAACACCTTCGTGTCTGG GGGTTGTGACAAGAAGGCCATGGTGTGGGACATGCGCTCTGGCCAGTGCGTGCAAGCCTTTGAAACTCAT GAATCGGATGTCAACAGTGTTCGGTACTACCCCAGTGGGGATGCTTTTGCTTCGGGGTCGGATGATGCCA CGTGTCGCCTCTATGACCTGAGGGCAGATAGAGAGGTCGCCATCTACTCCAAGGAGAGCATCATATTTGG CGCCTCGAGTGTGGACTTCTCCCTCAGTGGTCGCCTGCTTTTTGCTGGGTACAATGACTATACCATCAAT GTCTGGGATGTTCTCAAGGGCTCCCGGGTCTCCATCCTGTTTGGACATGAAAACCGCGTCAGCACTCTGC GAGTGTCTCCTGATGGGACAGCATTCTGCTCGGGATCATGGGATCACACCCTAAGAGTTTGGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206192 protein sequence
Red=Cloning site Green=Tags(s) MCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLNYCSTCAEIMATDGLHENETLASLKSEAESLKGKLEE ERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKE HAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQI LTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETH ESDVNSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTIN VWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010313 |
| ORF Size | 1188 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010313.2 |
| RefSeq Size | 2249 bp |
| RefSeq ORF | 1188 bp |
| Locus ID | 14697 |
| UniProt ID | P62881 |
| MW | 43.6 kDa |
| Gene Summary | Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors (By similarity). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201261 | Gnb5 (untagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1, (10ug) |
CNY 3,656.00 |
|
| MG206192 | Gnb5 (tGFP-tagged) - Mouse guanine nucleotide binding protein, beta 5 (Gnb5), transcript variant 1 |
CNY 3,710.00 |
|
| MR206192L3 | Lenti ORF clone of Gnb5 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1 |
CNY 5,230.00 |
|
| MR206192L4 | Lenti ORF clone of Gnb5 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), beta 5 (Gnb5), transcript variant 1 |
CNY 5,230.00 |
