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Ppm1a (NM_008910) Mouse Tagged ORF Clone
CAT＃: MR205969
- TrueORF®
Ppm1a (Myc-DDK-tagged) - Mouse protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a)
ORF Plasmid: tGFP
"NM_008910" in other vectors (4)
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CNY 3,330.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310003C21Rik; 2900017D14Rik; AI427932; AU017636; MMPa-2; MPPa-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205969 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCATTTTTAGACAAGCCAAAGATGGAGAAGCATAATGCCCAGGGGCAGGGGAATGGGTTACGAT ACGGCCTAAGCAGCATGCAAGGTTGGCGAGTTGAAATGGAGGACGCACACACGGCTGTGATCGGTTTGCC AAGTGGACTTGAGACATGGTCATTCTTTGCTGTATATGATGGGCATGCTGGTTCTCAGGTTGCCAAATAC TGCTGTGAGCACTTGTTAGATCACATCACCAATAACCAGGATTTCAGAGGATCTGCAGGAGCACCTTCTG TGGAGAACGTAAAGAATGGAATCAGAACAGGGTTTCTGGAGATTGATGAACACATGAGAGTTATGTCAGA GAAGAAACATGGTGCAGATAGAAGCGGGTCAACAGCTGTGGGCGTCTTAATCTCTCCCCAACATACTTAT TTCATTAACTGTGGAGACTCGAGAGGTTTACTTTGTAGGAATAGAAAAGTTCACTTCTTCACACAAGACC ATAAACCAAGTAACCCGCTGGAAAAAGAACGAATTCAGAATGCAGGGGGCTCGGTGATGATTCAGCGTGT CAATGGCTCTCTGGCTGTATCGAGGGCCCTTGGGGATTTCGATTACAAATGTGTCCATGGAAAAGGTCCC ACAGAGCAGCTTGTCTCCCCAGAGCCCGAAGTCCATGATATTGAAAGGTCTGAAGAAGATGACCAGTTCA TCATCCTTGCATGCGATGGCATCTGGGACGTCATGGGGAACGAAGAGCTCTGTGACTTTGTGAGATCCAG ACTTGAAGTCACTGATGACCTTGAGAAAGTTTGCAATGAAGTAGTCGACACCTGCTTGTATAAGGGAAGT CGAGACAACATGAGTGTGATTTTGATCTGTTTTCCAAGTGCACCCAAAGTCTCGGCAGAGGCGGTGAAGA AGGAGGCGGAGCTGGACAAGTACCTGGAGAGCAGAGTAGAAGAAATCATAAAGAAGCAGGTGGAAGGCGT CCCTGACTTAGTCCACGTGATGCGCACGTTAGCCAGTGAGAACATCCCCAGCCTCCCACCAGGGGGTGAA TTGGCAAGCAAGCGGAATGTAATTGAAGCCGTTTACAATAGACTGAACCCTTACAAAAATGACGACACTG ATTCTGCGTCAACCGATGATATGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205969 protein sequence
Red=Cloning site Green=Tags(s) MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLETWSFFAVYDGHAGSQVAKY CCEHLLDHITNNQDFRGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTY FINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGP TEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGS RDNMSVILICFPSAPKVSAEAVKKEAELDKYLESRVEEIIKKQVEGVPDLVHVMRTLASENIPSLPPGGE LASKRNVIEAVYNRLNPYKNDDTDSASTDDMW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008910 |
| ORF Size | 1149 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008910.3 |
| RefSeq Size | 2789 bp |
| RefSeq ORF | 1149 bp |
| Locus ID | 19042 |
| UniProt ID | P49443 |
| MW | 42.4 kDa |
| Gene Summary | Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation
,Wang, Z;Ji, N;Chen, Z;Sun, Z;Wu, C;Yu, W;Hu, F;Huang, M;Zhang, M;,
Allergy Asthma Immunol Res
,PubMed ID 32638565
[PPM1A]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205648 | Ppm1a (untagged) - Mouse protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a), (10ug) |
CNY 2,000.00 |
|
| MG205969 | Ppm1a (tGFP-tagged) - Mouse protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a) |
CNY 3,710.00 |
|
| MR205969L3 | Lenti ORF clone of Ppm1a (Myc-DDK-tagged) - Mouse protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a) |
CNY 5,230.00 |
|
| MR205969L4 | Lenti ORF clone of Ppm1a (mGFP-tagged) - Mouse protein phosphatase 1A, magnesium dependent, alpha isoform (Ppm1a) |
CNY 5,230.00 |
