Ndrg1 (NM_008681) Mouse Tagged ORF Clone

CAT#: MR205950

  • TrueORF®

Ndrg1 (Myc-DDK-tagged) - Mouse N-myc downstream regulated gene 1 (Ndrg1)

ORF Plasmid: DDK tGFP



  "NM_008681" in other vectors (6)

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Mouse Protein / Lysate Request

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


NDRG1 Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms CAP43; CMT4D; DRG1; HMSNL; Ndr1; Ndrl; NMSL; PROXY1; RTP; TDD5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205950 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGAGAGCTACATGACGTGGACCTCGCTGAGGTGAAGCCTCTGGTGGAAAAAGGGGAGAGCATCA
CTGGTCTCCTGCAAGAGTTCGATGTTCAGGAGCAGGATATTGAGACCCTACATGGCTCCCTGCATGTCAC
CCTGTGTGGGACCCCCAAGGGCAACCGTCCTGTCATCCTCACGTATCACGACATCGGCATGAACCACAAG
ACCTGCTACAACCCCCTCTTCAACTCTGAGGACATGCAGGAGATCACACAACATTTTGCTGTCTGCCATG
TGGATGCCCCTGGCCAACAGGATGGTGCCCCTTCCTTCCCAGTGGGGTACATGTACCCCTCAATGGATCA
GTTGGCTGAAATGCTTCCTGGAGTCCTTCACCAGTTTGGGCTCAAGAGTGTCATTGGCATGGGGACAGGA
GCTGGCGCCTACATCCTGACCCGCTTCGCACTCAACAACCCTGAGATGGTAGAGGGTCTCGTGCTCATGA
ATGTGAACCCCTGTGCTGAAGGCTGGATGGACTGGGCTGCCTCTAAGATCTCAGGATGGACCCAAGCCCT
GCCTGACATGGTGGTGTCCCACCTCTTCGGCAAGGAGGAGATACACAACAACGTGGAGGTAGTGCACACG
TACCGCCAGCACATCCTCAACGACATGAACCCGAGCAACCTACACTTATTCATCAGCGCCTACAACAGCC
GGAGAGACCTGGAGATTGAGCGGCCAATGCCTGGAACCCACACTGTCACTTTGCAGTGCCCTGCTCTGCT
GGTAGTTGGAGACAACTCTCCTGCCGTGGATGCTGTGGTGGAATGCAATTCTAAATTGGACCCAACGAAG
ACCACCCTGCTCAAGATGGCAGACTGTGGAGGCCTTCCTCAGATCTCTCAGCCCGCCAAGCTTGCTGAGG
CCTTCAAGTACTTTGTGCAGGGCATGGGATACATGCCTTCTGCCAGCATGACTCGCCTGATGCGGTCCCG
CACAGCCTCTGGCTCCAGTGTCACATCCCTGGAGGGCACCCGTAGCCGCTCCCATACCAGCGAGGGCCCC
CGAAGCCGCTCGCACACCAGTGAGGGGTCCCGTAGCCGCTCCCATACCAGTGAGGATGCCCGTCTCAACA
TCACCCCCAACTCGGGTGCCACCGGAAACAATGCCGGGCCCAAGTCCATGGAGGTGTCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205950 protein sequence
Red=Cloning site Green=Tags(s)

MSRELHDVDLAEVKPLVEKGESITGLLQEFDVQEQDIETLHGSLHVTLCGTPKGNRPVILTYHDIGMNHK
TCYNPLFNSEDMQEITQHFAVCHVDAPGQQDGAPSFPVGYMYPSMDQLAEMLPGVLHQFGLKSVIGMGTG
AGAYILTRFALNNPEMVEGLVLMNVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEIHNNVEVVHT
YRQHILNDMNPSNLHLFISAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDNSPAVDAVVECNSKLDPTK
TTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLEGTRSRSHTSEGP
RSRSHTSEGSRSRSHTSEDARLNITPNSGATGNNAGPKSMEVSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008681
ORF Size 1185 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008681.2, NP_032707.2
RefSeq Size 2909 bp
RefSeq ORF 1185 bp
Locus ID 17988
UniProt ID Q62433
MW 43 kDa
Gene Summary Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy (By similarity). Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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