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Dnajb12 (NM_019965) Mouse Tagged ORF Clone
CAT＃: MR205857
- TrueORF®
Dnajb12 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12)
ORF Plasmid: tGFP
"NM_019965" in other vectors (5)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Dj10; mDj10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCCAACAAGGATGAAGCCGAGCGCTGTATCAGCATCGCCCTCAAGGCCATCCAAAGCAACCAGC CCGAGCGGGCGCTGCGCTTCCTGGAGAAGGCGCAGCGGCTCTACCCGACGCCACGAGTCAGCGCACTGAT CGAGTCCCTCAACCAAAAACCACAGTCCACCGGTGATCACCCTCAACCCACAGACACTACCCATACCACC ACCAAGAAGGCAGGCGGGACCGAAACCCCCTCAGCCAACGGCGAGGCAGGAGGAGGGGAGAGCGCCAAGG GCTATACCTCAGAGCAGGTGGCAGCTGTGAAAAGGGTCAAGCAGTGTAAAGATTACTATGAGATCCTTGG GGTAAGCAGAAGTGCCTCAGATGAGGACCTAAAGAAGGCCTACCGCAAGCTGGCCCTCAAGTTCCATCCA GACAAGAACCATGCGCCTGGGGCCACTGAAGCTTTCAAAGCCATTGGCACAGCGTATGCCGTACTCAGCA ACCCAGAGAAAAGGAAACAGTACGACCAGTTTGGTGATGACAAGAGCCAGGCTGCCCGGCACGGCCACAG CCACGGGGACTTCCACCGAGGCTTTGAGGCTGACATCTCCCCAGAAGACCTCTTTAACATGTTCTTTGGT GGCGGCTTTCCTTCCAGTAACGTTCATGTCTACAGCAATGGCCGAATGCGGTACACCTACCAGCAGAGGC AAGACCGCAGAGACAACCAGGGTGATGGCGGGCTAGGAGTGTTCGTGCAGCTGATGCCCATCCTCATCCT CATCCTCGTGTCTGCGCTCAGCCAGCTCATGGTGTCCAGTCCTCCCTACAGCCTGAGCCCGAGACCGTCA GTGGGCCACATCCACAAGCGAGTCACTGACCACCTGAACGTCGCCTACTACGTGGCAGACACCTTCTCCG AAGAGTACACGGGCTCCAGCCTCAAAACAGTGGAACGGAATGTAGAGGATGACTACATCGCCAACCTGCG CAACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGCTGTACCGAGCCCGCTACTTTGGTGACACA GACATGTACCACAGAGCACAGAAGATGGGCACCCCGAGTTGCAATCGGCTGTCAGAGGTGCAGGCCTCCC TGCATGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205857 protein sequence
Red=Cloning site Green=Tags(s) MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESLNQKPQSTGDHPQPTDTTHTT TKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILGVSRSASDEDLKKAYRKLALKFHP DKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHSHGDFHRGFEADISPEDLFNMFFG GGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPS VGHIHKRVTDHLNVAYYVADTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDT DMYHRAQKMGTPSCNRLSEVQASLHG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019965 |
| ORF Size | 1131 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019965.3 |
| RefSeq Size | 1789 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 56709 |
| UniProt ID | Q9QYI4 |
| MW | 42 kDa |
| Gene Summary | Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201823 | Dnajb12 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12), (10ug) |
CNY 3,656.00 |
|
| MC201824 | Dnajb12 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12), (10ug) |
CNY 3,656.00 |
|
| MG205857 | Dnajb12 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) |
CNY 3,140.00 |
|
| MR205857L3 | Lenti ORF clone of Dnajb12 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) |
CNY 4,750.00 |
|
| MR205857L4 | Lenti ORF clone of Dnajb12 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily B, member 12 (Dnajb12) |
CNY 4,750.00 |
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